Genotyping bovine leukemia virus in dairy cattle of Heilongjiang, northeastern China

Yu, Changqing; Wang, Xuefeng; Zhou, Youwen; Wang, Yu; Zhang, Xianfeng; Zheng, Yong Hui

doi:10.1186/s12917-019-1863-3

Cited by 31 publications

(42 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…An ML phylogenetic tree was constructed using the best K2+I model of nucleotide substitution ( 33 ). The results of phylogenetic analysis were similar to those published in previous studies and BLV strains were classified into 11 genotypes ( 3 , 18 , 29 , 34 – 36 ), as shown in Figure 2 . Interestingly, our phylogenetic analysis also showed that the Egyptian BLV isolates were clustered into genotype-1 or -4.…”

Section: Resultssupporting

confidence: 86%

“…In contrast to gp51, gp30 is poorly immunogenic ( 1 ) and therefore, BLV env- gp51 sequencing is mainly used for BLV phylogenetic studies ( 3 ). At least 11 BLV genotypes have been identified worldwide based on the phylogenetic analysis of env -gp51 sequences of BLV after identifying BLV genotype-11 in China ( 3 , 18 ). Three genotypes of BLV, namely genotype-1,-4, and-6, has been mainly detected worldwide ( 3 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Detection and Molecular Characterization of Bovine Leukemia Virus in Egyptian Dairy Cattle

Hamada

Metwally

Polat³

et al. 2020

Front. Vet. Sci.

View full text Add to dashboard Cite

Bovine leukemia virus (BLV) causes enzootic bovine leukosis (EBL), the most common neoplastic disease in cattle worldwide. The first EBL outbreak in Egypt was reported in 1997. To date, there are few studies regarding BLV diagnosis using only serological detection and no studies investigating the distribution of BLV provirus, which is the retroviral genome integrated into the host genome, in Egypt. The genetic characteristics of Egyptian BLV strains are also unknown. Therefore, we aimed to detect BLV provirus and determine BLV genetic variability among dairy cattle in Egypt. We collected 270 blood samples of dairy cattle from 24 farms located in five provinces in Egypt. Out of the 270 samples, 58 (21.5%) were positive for BLV provirus. Phylogenetic analysis based on 18 420-bp selected sequences out of 50 isolates of the BLV env -gp51 gene demonstrated that Egyptian BLV isolates were clustered into genotype-1 and-4, among 11 genotypes detected worldwide. Furthermore, phylogenetic analysis and alignment of the 501-bp sequence of the env- gp51 gene revealed that at least six genetically different strains are present in Egypt. Genotype-1 isolates comprised four different strains (G1-a, G1-b, G1-c, and G1-d) and genotype-4 isolates included two different strains (G4-x and G4-y). Moreover, in one farm with 100% infection rate, we identified three isolates of G1-a strain, 35 isolates of G4-x strain, and two isolates of G4-y strain. Overall, this study provides the new report on molecular prevalence of BLV in Egypt and records the coexistence of BLV genotype-1 and-4 in Egyptian cattle.

show abstract

Section: Resultssupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Detection and Molecular Characterization of Bovine Leukemia Virus in Egyptian Dairy Cattle

Hamada

Metwally

Polat³

et al. 2020

Front. Vet. Sci.

View full text Add to dashboard Cite

show abstract

“…However, most countries are still confronting the burden of BLV infection. Across Asian countries, BLV infection rate remains a wide range from 3.9% to 70% among Japan [ 29 ], Korea [ 19 ], China [ 42 , 43 ], Taiwan [ 41 ], Thailand [ 20 ], Philippines [ 32 ], Cambodia [ 24 ], Mongolia [ 28 ], Myanmar [ 31 ], and Vietnam [ 9 ].…”

mentioning

confidence: 99%

“…The env gene encodes the envelope protein complex composing gp51 surface glycoprotein (SU) and gp30 transmembrane (TM) protein. As the env-gp51 plays an essential and indispensable role for viral life cycle and viral infectivity, such as cell entry and production of neutralizing antibodies [ 17 , 21 ], the env-gp51 gene had been widely become a target gene for diagnosis, molecular characterization and genotyping of BLV [ 12 , 19 , 20 , 32 , 34 , 43 ]. Based on the analysis of gp51 gene sequences, Rodriguez et al demonstrated that BLV strains can be classified into 7 distinct genotypes [ 39 ].…”

mentioning

confidence: 99%

Detection and genotyping of bovine leukemia virus (BLV) in Vietnamese cattle

Yamashita‐Kawanishi

Okamoto

et al. 2020

J. Vet. Med. Sci.

View full text Add to dashboard Cite

Bovine leukemia virus (BLV) belongs to the genus, Deltaretrovirus of the family, Retroviridae and it is the causative agent of enzootic bovine leukosis. The prevalence of BLV in three provinces in the Red River Delta Region in the North of Vietnam, Hanoi, Vinhphuc and Bacninh was studied from April 2017 to June 2018. A total of 275 blood samples collected from cattle were used for serum isolation and DNA extraction. Of these samples, 266 sera were subjected to ELISA test for detecting antibody against BLV gp51 protein and 152 DNA samples were used to detect the 444 bp fragment corresponding to a part of the gp51 region of the env by nested PCR. The results showed that 16.5% (n=44) and 21.1% (n=32) of samples were positive for BLV gp51 antibody and BLV proviral DNA, respectively. Phylogenetic analysis of the partial (423 bp) and complete (913 bp) BLV env-gp51 gene indicated that Vietnamese strains were clustered into genotypes 1, 6 and 10 (G1, G6 and G10). Of those genotypes, G1 genotype was dominant; G6 strains were designated as G6e and G6f subgenotypes; the existence of genotype 10 was confirmed for the first time in Vietnam. The present study provides important information regarding the prevalence of BLV infection and genetic characteristics of BLV strains identified in Vietnam, contributing to promote the establishment of disease control and eradication strategies in Vietnam.

show abstract

“…Bovine leukemia is a widespread viral pathology among livestock in many countries of the world. [1][2][3][4] The bovine leukemia virus (BLV) is able to suppress the general immune resistance of infected animals by destroying the genetic potential of the animals. One of the critical issues is the search and implementation of effective and affordable biologically active substances that increase immunological resistance and productive qualities in the leukemia control system.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of Immunological Parameters of Cattle Infected with Leukemia Virus and its Correction with Betulin–Based Medication

Красиков¹,

Vlasenko²,

Плешакова³

et al. 2020

Proceedings of the International Scientific Conference the Fifth Technological Order: Prospects for the Development and Moderni

View full text Add to dashboard Cite

The paper presents the results indicating the possibility of using betulin-based medication for the correction of the immune status of cattle infected with bovine leukemia virus (BLV). To set up the experiment, we designed 3 prototypes of the medication (betulin-B1, B2 and B3), which were tested on 20 cattle units that reacted positively in RID test with the glycoprotein antigen of BLV. 15 experimental animals were formed into 3 groups. Cows of the 1st group (n = 5) were injected subcutaneously with the betulin-B1 preparation three times with an interval of 7 days at a dose of 0.2 ml per 1 kg of live weight, groups 2 and 3 (n = 5) were injected with betulin-B2 and B3, respectively, in the same method. Another five RID-positive cows were used as a control group. After the first and the third administration of medications, hematological and immunological studies were performed. Immunological restructuring of the body after the use of betulin preparations B1 and B3 was characterized by a decrease in the number of lymphocytes due to the population of B-lymphocytes and Tcytotoxic lymphocytes; a decrease in the content of circulating immune complexes, an increase in the functional and metabolic activity of neutrophils, bactericidal and lysozyme activity of blood serum and balance of the protein spectrum. Thus, the revealed changes in the hematological and immunological parameters prove the effectiveness and feasibility of using betulin medication for the correction of the immune status of cattle infected with BLV.

show abstract

Genotyping bovine leukemia virus in dairy cattle of Heilongjiang, northeastern China

Cited by 31 publications

References 46 publications

Detection and Molecular Characterization of Bovine Leukemia Virus in Egyptian Dairy Cattle

Detection and Molecular Characterization of Bovine Leukemia Virus in Egyptian Dairy Cattle

Detection and genotyping of bovine leukemia virus (BLV) in Vietnamese cattle

Dynamics of Immunological Parameters of Cattle Infected with Leukemia Virus and its Correction with Betulin–Based Medication

Contact Info

Product

Resources

About