2022
DOI: 10.1007/978-1-0716-2333-6_8
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Genotyping of African Swine Fever Virus

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Cited by 2 publications
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“…Surprisingly, pairwise Geneious Prime (2023.2.1) alignment revealed that the B646L gene, which encodes for the major capsid protein p72, was identical to the genotype VIII reference strain Malawi Lil 20/1 (AY261361). Therefore, we Sanger sequenced ( 3 ) the original biobanked sample from the Pirbright reference collection of NAM P1/1995 and identified ambiguities consistent with a mixed population of genotype I and genotype VIII viruses. Virus cultures containing genotype I (clone 23) and genotype VIII (clone 3) viruses were generated by limit dilution ( 2 ) and the B646L sequences from the two clones compared to the original NAM P1/1995 B646L sequence DQ250122 ( Fig.…”
Section: Announcementmentioning
confidence: 99%
“…Surprisingly, pairwise Geneious Prime (2023.2.1) alignment revealed that the B646L gene, which encodes for the major capsid protein p72, was identical to the genotype VIII reference strain Malawi Lil 20/1 (AY261361). Therefore, we Sanger sequenced ( 3 ) the original biobanked sample from the Pirbright reference collection of NAM P1/1995 and identified ambiguities consistent with a mixed population of genotype I and genotype VIII viruses. Virus cultures containing genotype I (clone 23) and genotype VIII (clone 3) viruses were generated by limit dilution ( 2 ) and the B646L sequences from the two clones compared to the original NAM P1/1995 B646L sequence DQ250122 ( Fig.…”
Section: Announcementmentioning
confidence: 99%