Genotyping of bovine viral diarrhea viruses from cattle in China between 2005 and 2008

Xue, Feiqun; Zhu, Yuanmao; Li, Jiao; Zhu, L Q; Ren, Xiangang; Feng, Jie; Shi, Hengzhen; Gao, Yuwei

doi:10.1016/j.vetmic.2009.11.010

Cited by 70 publications

(68 citation statements)

References 17 publications

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“…EU159702). 10 This is consistent with type 1c being the most prevalent strain of BVDV in Australia. 9 Each dam was fitted with a custom-made udder cover ( Fig.…”

supporting

confidence: 85%

Comparison of serum, ear notches, and nasal and saliva swabs for Bovine viral diarrhea virus antigen detection in colostrum-fed persistently infected (PI) calves and non-PI calves

et al. 2014

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The diagnosis of neonatal and young calves persistently infected (PI) with Bovine viral diarrhea virus (BVDV) by antigen-capture enzyme-linked immunosorbent assay (ACE) may be complicated by interference from colostrum-derived specific antibodies. Ten calves, with 3 calves identified as PI and 7 as non-PI were used in the current study. All non-PI calves were shown to be seropositive for BVDV-specific antibodies by antibody enzyme-linked immunosorbent assay (Ab-ELISA) on serum. Serum samples, ear notch samples, and nasal and saliva swabs were collected from each calf from birth until 12 weeks of age and tested by ELISA for BVDV-specific antigen and antibodies. Following colostrum ingestion, Ab-ELISA sample-to-positive (S/P) ratios rose by a mean of 0.95 (95% confidence interval [CI] = 0.64-1.25) and 1.72 (95% CI = 1.55-1.89) in seropositive, non-PI calves and in PI calves, respectively. The mean S/P ratios then declined to approximately 1.1 in non-PI calves and 0.5 in PI calves at between 60 and 80 days of age. In PI calves, testing for antigen in serum and nasal and saliva swabs was subject to interference by colostrum-derived antibodies in calves up to 3 weeks of age. Nasal swabs were less affected than serum and saliva swabs. Ear notches maintained positive ACE corrected optical densities at all sample times, despite a drop in the signal following the ingestion of colostrum.

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“…EU159702). 10 This is consistent with type 1c being the most prevalent strain of BVDV in Australia. 9 Each dam was fitted with a custom-made udder cover ( Fig.…”

supporting

confidence: 85%

Comparison of serum, ear notches, and nasal and saliva swabs for Bovine viral diarrhea virus antigen detection in colostrum-fed persistently infected (PI) calves and non-PI calves

et al. 2014

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“…Recently, a third BVDV species, called HoBi-like virus or BVDV3, was reported [4]. BVDV-1 is classified by genetic analysis into about 20 subtypes (1a-1t) [5][6][7][8][9][10] and BVDV-2 into three subtypes (2a-2c) [11][12][13]. BVDV subtyping is useful in molecular epidemiological studies, vaccine development and tracing the origin of emerging viruses [14].…”

Section: Introductionmentioning

confidence: 99%

Circulation of bovine viral diarrhea virus – 1 (BVDV-1) in dairy cattle and buffalo farms in Ismailia Province, Egypt

Soltan

Wilkes

Elsheery

et al. 2015

J Infect Dev Ctries

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Introduction: Bovine viral diarrhea (BVD) is one of the most economically significant diseases in the bovine industry causing losses due to diarrhea, reproductive disorders, immunosuppression and mortalities. The aim of our investigation was to detect and subtype BVDV from calves on two dairy cattle and two buffalo farms in Ismailia province, Egypt as an indicator of BVDV infection status in the province. Methodology: A total of 298 blood samples were collected and tested using an optimized one-step, real-time multiplex Taqman-based RT-PCR. All the positive samples by the multiplex real-time RT-PCR were tested using conventional RT-PCR to amplify multiple areas of the genome for further phylogenetic analysis and subtyping. Results: Thirty one (10.4%) of the tested samples were positive for BVDV-1. Only three samples, all from a single dairy cattle farm, had enough viral RNA to be amplified by RT-PCR. The PCR products were sequenced and phylogenetic analysis revealed detection of BVDV1b. The detected strain is closely related to worldwide BVDV-1b strains, making it difficult to trace its origin. Nucleotide and amino acid alignments of the E2 glycoprotein region of the detected strain with other BVDV-1b strains showed high divergence, with identity ranging from 81.3% to 93.6% and 85.3% to 93.6%, respectively. Conclusion: To our knowledge, this is the first report describing the circulation of BVDV-1b in Egyptian dairy cattle populations.

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“…Sequencing of the 5 UTR region confirmed that the BVDV strain persisting in the PI cow from which the inoculating serum was collected showed 98% homology with BVDV type 1c strains deposited with GenBank (GenBank accession number: EU159702) (Xue et al, 2010). There was also 99.6% homology between the sequences of the PI cow and L14, with a single nucleotide polymorphism on the 212th position of the 292 bp UTR region sequenced, where the cytosine was substituted for a thyamine.…”

Section: Genotype Of Bvdv Strainmentioning

confidence: 72%

Reproductive performance in experimentally BVDV infected ewes and seroconversion rates in sheep co-mingled with BVDV PI calves

Lanyon

Sims

et al. 2015

Small Ruminant Research

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Genotyping of bovine viral diarrhea viruses from cattle in China between 2005 and 2008

Cited by 70 publications

References 17 publications

Comparison of serum, ear notches, and nasal and saliva swabs for Bovine viral diarrhea virus antigen detection in colostrum-fed persistently infected (PI) calves and non-PI calves

Comparison of serum, ear notches, and nasal and saliva swabs for Bovine viral diarrhea virus antigen detection in colostrum-fed persistently infected (PI) calves and non-PI calves

Circulation of bovine viral diarrhea virus – 1 (BVDV-1) in dairy cattle and buffalo farms in Ismailia Province, Egypt

Reproductive performance in experimentally BVDV infected ewes and seroconversion rates in sheep co-mingled with BVDV PI calves

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