Genotyping of Campylobacter coli and C. jejuni from retail chicken meat and humans with campylobacteriosis in Slovenia and Bosnia and Herzegovina

Zorman, Tina; Heyndrickx, Marc; Uzunović-Kamberović, Selma; Možina, Sonja Smole

doi:10.1016/j.ijfoodmicro.2006.03.001

Cited by 40 publications

(33 citation statements)

References 42 publications

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“…Stern and Kazmi (1989) stated already over two decades ago that a large number of C. jejuni serotypes from poultry can frequently be linked to human cases of campylobacteriosis. This was confirmed by later reports using also genetic typing techniques (Zorman et al 2006, Gonzalez et al 2009, Mullner et al 2010. In 1997 in Denmark, 36% of broilers were colonized by C. jejuni and a large overlap of the most common serotypes in humans and broiler chickens was shown (Nielsen et al 1997).…”

Section: Contribution Of the Chicken Reservoir To Campylobacteriosis mentioning

confidence: 58%

Poultry as a Host for the Zoonotic PathogenCampylobacter jejuni

Hermans

Pasmans²,

Messens³

et al. 2012

Vector-Borne and Zoonotic Diseases

223

157

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Campylobacteriosis is the most reported foodborne gastroenteritic disease and poses a serious health burden in industrialized countries. Disease in humans is mainly caused by the zoonotic pathogen Campylobacter jejuni. Due to its wide-spread occurrence in the environment, the epidemiology of Campylobacter remains poorly understood. It is generally accepted, however, that chickens are a natural host for Campylobacter jejuni, and for Campylobacter spp. in general, and that colonized broiler chicks are the primary vector for transmitting this pathogen to humans. Several potential sources and vectors for transmitting C. jejuni to broiler flocks have been identified. Initially, one or a few broilers can become colonized at an age of > 2 weeks until the end of rearing, after which the infection will rapidly spread throughout the entire flock. Such a flock is generally colonized until slaughter and infected birds carry a very high C. jejuni load in their gastrointestinal tract, especially the ceca. This eventually results in contaminated carcasses during processing, which can transmit this pathogen to humans. Recent genetic typing studies showed that chicken isolates can frequently be linked to human clinical cases of Campylobacter enteritis. However, despite the increasing evidence that the chicken reservoir is the number one risk factor for disease in humans, no effective strategy exists to reduce Campylobachter prevalence in poultry flocks, which can in part be explained by the incomplete understanding of the epidemiology of C. jejuni in broiler flocks. As a result, the number of human campylobacteriosis cases associated with the chicken vector remains strikingly high.

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Section: Contribution Of the Chicken Reservoir To Campylobacteriosis mentioning

confidence: 58%

Poultry as a Host for the Zoonotic PathogenCampylobacter jejuni

Hermans

Pasmans²,

Messens³

et al. 2012

Vector-Borne and Zoonotic Diseases

223

157

View full text Add to dashboard Cite

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“…which was optimized for Campylobacter strains (22). For PFGE typing, the cells were cultured on Mueller Hinton agar and agarose blocks, prepared using INCERT agarose (FMC Bio Products, Rockland, ME, USA).…”

Section: Fla-rflp Typing and Pfge Typingmentioning

confidence: 99%

Role of Poultry Meat in Sporadic Campylobacter Infections in Bosnia and Herzegovina: Laboratory-based Study

et al. 2007

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Aim To investigate genetic diversity and specificity of Campylobacter jejuni and Campylobacter coli strains isolated from humans, retail poultry meat, and live farm chickens in Zenica-Doboj Canton, Bosnia and Herzegovina, and identify the role of poultry meat in sporadic Campylobacter infections.Methods We determined the type of Campylobacter species using standard microbiological methods and multiplex polymerase chain reaction (PCR), and performed pulsed field gel-electrophoresis (PFGE) and restriction fragment length polymorphism (RFLP) typing of the flaA gene to investigate genetic diversity among the isolates.Results We isolated C jejuni and C coli from 75 (5.2%) of 1453 samples of consecutive outpatients with sporadic diarrhea; from 51 (34.7%) of 147 samples of poultry meat; and from 15 out of 23 farm chicken samples. The proportion of C coli found among human (30.1%), poultry meat (56.9%), and farm chicken isolates (53.3%), was greater than the proportion of C jejuni. Fourteen and 24 PFGE genotypes were identified among 20 C coli and 37 C jejuni isolates, respectively. Identical PFGE genotypes were found in two cases of human and poultry meat isolates and two cases of poultry meat and farm chicken isolates.Conclusion Only a minority of human Campylobacter isolates shared identical PFGE type with poultry meat isolates. Although poultry is the source of a certain number of human infections, there may be other more important sources. Further research is required to identify the environmental reservoir of Campylobacter spp responsible for causing human disease and the reason for the high prevalence of C coli human infections in this region.

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“…The cecal microbiome plays an important role in fermentation processes and production of short chain fatty acids (SCFA) (Patterson and Burkholder, 2003;Rehman et al, 2007). Also, the cecum can host pathogenic and zoonotic bacteria that cause severe risks to human health (Herman et al, 2003;Zorman et al, 2006).…”

Section: Introductionmentioning

confidence: 99%