Genotyping of Candida albicans by Fragment Analysis of Microsatellites Combined with 25S rDNA and RPS-based Strategies

Adachi, Hidesada; Shimizu, Kazuharu; Hattori, Hisao; Tanaka, Reiko; Chibana, Hiroji; Takagi, Yuki; Tomita, Yasushi; Kanbe, Toshio

doi:10.3314/jjmm.50.167

Cited by 15 publications

(9 citation statements)

References 23 publications

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“…Interestingly, for each of the 11 microsatellites analyzed, the number of alleles identified per locus was similar to that described by other authors (Botterel et al, 2001;Fundyga et al, 2002;Sampaio et al, 2005) (Supplemental Table 2), ranging from 5 (for LOC4) to 44 (for CAI) different alleles. The DP index at each of the 11 microsatellites tested showed HIS3 and CAI microsatellites as being the most discriminant markers (Supplemental Table 2) with the highest number of alleles identified, corroborating similar observations (Adachi et al, 2009).…”

Section: Locussupporting

confidence: 80%

“…The MLMT analysis of 174 C. albicans isolates previously characterized at the CEF3 microsatellite (Dalle et al, 2003(Dalle et al, , 2000 gave a 100% typability to the method, with a DP index of 0.999 that was higher or similar to those obtained by other studies based on MLMT approaches (Adachi et al, 2009;Chavez-Galarza et al, 2010;Dalle et al, 2008;Garcia-Hermoso et al, 2007). Interestingly microsatellites with the highest DP index were not necessary the most powerful in their clustering abilities (as exemplified with HIS3 microsatellite that displayed a DP index of 0.997 when its Table 1).…”

Section: Discussionmentioning

confidence: 52%

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Using a Multi-Locus Microsatellite Typing method improved phylogenetic distribution of Candida albicans isolates but failed to demonstrate association of some genotype with the commensal or clinical origin of the isolates

L’Ollivier

Labruère

Jébrane

et al. 2012

Infection, Genetics and Evolution

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Section: Locussupporting

confidence: 80%

Section: Discussionmentioning

confidence: 52%

Using a Multi-Locus Microsatellite Typing method improved phylogenetic distribution of Candida albicans isolates but failed to demonstrate association of some genotype with the commensal or clinical origin of the isolates

L’Ollivier

Labruère

Jébrane

et al. 2012

Infection, Genetics and Evolution

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“…In microsatellite analysis we were able to verify the presence of six different alleles with the primer CDC3 and seven alleles with primer HIS3, of which 113bp, 117bp, 125bp, 150bp, 154bp, 158bp and 162bp have already been recognized by other authors 1 6 7 . These primers amplify microsatellite regions highly polymorphic for C. albicans .…”

Section: Discussionmentioning

confidence: 73%

“…The microsatellite analysis is a technique which has been recently used for genotyping C. albicans 1 6 7 . It is formed by short tandem repeats of two to six nucleotides known to be highly polymorphic, generating a characteristic profile of different alleles for a given locus .…”

Section: Introductionmentioning

confidence: 99%

MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS

Mendonca¹,

Fiorini²,

Shinobu-Mesquita³

et al. 2013

Rev. Inst. Med. trop. S. Paulo

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SUMMARY Introduction: The majority of nosocomial fungal infections are caused by Candida spp. where C. albicans is the species most commonly identified. Molecular methods are important tools for assessing the origin of the yeasts isolated in hospitals. Methods: This is a study on the genetic profifiles of 39 nosocomial clinical isolates of C. albicans using two typing methods: random amplifified polymorphic DNA (RAPD) and microsatellite, two different primers for each technique were used. Results: RAPD provided 10 and 11 different profiles with values for SAB of 0.84 ± 0.126 and 0.88 ± 0.08 for primers M2 and P4, respectively. Microsatellite using two markers, CDC3 and HIS3, allowed the observation of six and seven different alleles, respectively, with combined discriminatory power of 0.91. Conclusions: Although genetic variability is clear, it was possible to identify high similarity, suggesting a common origin for at least a part of isolates. It is important to emphasize that common origin was proven from yeasts isolated from colonization (urine, catheter or endotracheal secretions) and blood culture from the same patient, indicating that the candidemia must have started from a site of colonization. The combination of RAPD and microsatellite provides a quick and efficient analysis for investigation of similarity among nosocomial isolates of C. albicans.

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“…Hypoxia-related genes have been identified as important virulence factors for Aspergillus fumigatus and Candida albicans given that they are exposed to low oxygen concentrations during infection [93,94]. Oxygen levels in the secondary xylem, the plant tissue that is infected by C. cacaofunesta , are as low as are observed in human tissues that are infected by these fungal pathogens, reaching levels less than 5% [93,95].…”

Section: Resultsmentioning

confidence: 99%

Global analyses of Ceratocystis cacaofunesta mitochondria: from genome to proteome

et al. 2013

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BackgroundThe ascomycete fungus Ceratocystis cacaofunesta is the causal agent of wilt disease in cacao, which results in significant economic losses in the affected producing areas. Despite the economic importance of the Ceratocystis complex of species, no genomic data are available for any of its members. Given that mitochondria play important roles in fungal virulence and the susceptibility/resistance of fungi to fungicides, we performed the first functional analysis of this organelle in Ceratocystis using integrated “omics” approaches.ResultsThe C. cacaofunesta mitochondrial genome (mtDNA) consists of a single, 103,147-bp circular molecule, making this the second largest mtDNA among the Sordariomycetes. Bioinformatics analysis revealed the presence of 15 conserved genes and 37 intronic open reading frames in C. cacaofunesta mtDNA. Here, we predicted the mitochondrial proteome (mtProt) of C. cacaofunesta, which is comprised of 1,124 polypeptides - 52 proteins that are mitochondrially encoded and 1,072 that are nuclearly encoded. Transcriptome analysis revealed 33 probable novel genes. Comparisons among the Gene Ontology results of the predicted mtProt of C. cacaofunesta, Neurospora crassa and Saccharomyces cerevisiae revealed no significant differences. Moreover, C. cacaofunesta mitochondria were isolated, and the mtProt was subjected to mass spectrometric analysis. The experimental proteome validated 27% of the predicted mtProt. Our results confirmed the existence of 110 hypothetical proteins and 7 novel proteins of which 83 and 1, respectively, had putative mitochondrial localization.ConclusionsThe present study provides the first partial genomic analysis of a species of the Ceratocystis genus and the first predicted mitochondrial protein inventory of a phytopathogenic fungus. In addition to the known mitochondrial role in pathogenicity, our results demonstrated that the global function analysis of this organelle is similar in pathogenic and non-pathogenic fungi, suggesting that its relevance in the lifestyle of these organisms should be based on a small number of specific proteins and/or with respect to differential gene regulation. In this regard, particular interest should be directed towards mitochondrial proteins with unknown function and the novel protein that might be specific to this species. Further functional characterization of these proteins could enhance our understanding of the role of mitochondria in phytopathogenicity.

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Genotyping of Candida albicans by Fragment Analysis of Microsatellites Combined with 25S rDNA and RPS-based Strategies

Cited by 15 publications

References 23 publications

Using a Multi-Locus Microsatellite Typing method improved phylogenetic distribution of Candida albicans isolates but failed to demonstrate association of some genotype with the commensal or clinical origin of the isolates

Using a Multi-Locus Microsatellite Typing method improved phylogenetic distribution of Candida albicans isolates but failed to demonstrate association of some genotype with the commensal or clinical origin of the isolates

MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS

Global analyses of Ceratocystis cacaofunesta mitochondria: from genome to proteome

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