Genotyping of duck hepatitis virus type 3 isolated in Vietnam during 2009-2013

Roan, Do Thi; Hương, Đoàn Thị Thanh; Xuyen, Le Thi Kim; Khue, Nguyen Thi; Tien, Vu Thi; Le, Thanh Hoa

doi:10.15625/0866-7160/v37n1se.6122

Cited by 4 publications

(4 citation statements)

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“…Vietnam (Roan et al, 2015). Additionally, outbreaks with co-infections of DHAV-1 and DHAV-3 have recently been reported in mainland China and South Korea (Lin et al, 2016;Soliman et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, DHAV‐2 is endemic only in Taiwan (Tseng & Tsai, ). DHAV‐3 accounts for an increasing proportion of pathogens in South Korea (Cha, Roh, Kang, Kim, & Jang, ; Kim et al., ), mainland China (Liu et al., ) and Vietnam (Roan et al., ). Additionally, outbreaks with co‐infections of DHAV‐1 and DHAV‐3 have recently been reported in mainland China and South Korea (Lin et al., ; Soliman et al., ).…”

Section: Introductionmentioning

confidence: 99%

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Molecular epidemiology of duck hepatitis a virus types 1 and 3 in China, 2010-2015

Wen

Zhu

Cheng

et al. 2017

Transbound Emerg Dis

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Duck hepatitis A virus (DHAV) is the most common aetiologic agent of duck virus hepatitis (DVH), causing substantial economic losses in the duck industry worldwide. In China, officially approved DHAV-1 live-attenuated vaccines have been used widely to vaccinate breeder ducks since 2013. However, following the reports of DVH outbreaks, it has become necessary to assess the epidemiological situation of this virus in China. We conducted molecular epidemiological analyses of 32 DHAV field isolates while analysing the samples from ducks suspected of having hepatitis collected from commercial duck farms in China between May 2010 and December 2015. Considerable changes were observed in the epidemiology of DHAV-1 and DHAV-3 in China over time. A higher number of DHAV-1 strains were isolated during 2010-2012, coinciding with the widespread use of officially approved DHAV-1 live vaccine strains beginning in 2013. In contrast, a higher rate of DHAV-3 causing DHAV infections was observed between 2013 and 2015. Phylogenetic analyses based on the full-length VP1 gene were performed on these field isolates and using reference strains available in GenBank. DHAV-1 field isolates were evaluated in two groups: one group closely related to prototype strains and circulating in China between 2010 and 2012 and another group exhibiting genetic and serological differences from prototype strains. All DHAV-3 strains isolated in this study were grouped as monophyletic, which has become the predominant viral type, particularly in Shandong and Sichuan provinces, since 2013. In conclusion, these data provide updated information on the genetic and serological diversity of DHAV-1 and DHAV-3, and our findings may serve as a foundation for the prevention of, and vaccine development for, DHAV in China.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of duck hepatitis a virus types 1 and 3 in China, 2010-2015

Wen

Zhu

Cheng

et al. 2017

Transbound Emerg Dis

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“…The prevention of DVH mainly depends on the immunization of ducklings with commercial DHAV-1 live vaccines. However, the DHAV-1 vaccine failed to elicit cross-protection against DHAV-3, which currently severely affects Southeast Asia ( Kim et al, 2009 ; Roan et al, 2015 ). Furthermore, simultaneous co-infection with DHAV and DEV has become increasingly prevalent in domestic duck farms in China and South Korea ( Chen et al, 2013 ; Soliman et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

“…DHAV-1 infection is currently controlled using modified live attenuated vaccines ( Roh and Kang, 2018 ); in China, this has been officially approved for vaccinating breeder ducks since 2013. Although DHAV-3 accounts for an increasing proportion of DHAV infections in mainland China ( Liu et al, 2011b ), South Korea ( Kim et al, 2007 ), and Vietnam ( Roan et al, 2015 ), no commercial live vaccine suitable used in young ducklings is currently available. A safe and efficient vaccine for DHAV-3 is required.…”

Section: Introductionmentioning

confidence: 99%

Recombinant Duck Enteritis Virus-Vectored Bivalent Vaccine Effectively Protects Against Duck Hepatitis A Virus Infection in Ducks

Yang

Liu

et al. 2021

Front. Microbiol.

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Duck enteritis virus (DEV) and duck hepatitis A virus (DHAV) are prevalent duck pathogens, causing significant economic losses in the duck industry annually. Using a fosmid-based rescue system, we generated two DEV recombinants, rDEV-UL26/27-P13C and rDEV-US7/8-P13C, in which the P1 and 3C genes from DHAV type 3 (DHAV-3) were inserted into the DEV genome between genes UL26 and UL27 or genes US7 and US8. We inserted a self-cleaving 2A-element between P1 and 3C, allowing the production of both proteins from a single open reading frame. P1 and 3C were simultaneously expressed in infected chicken embryo fibroblasts, with no difference in growth kinetics between cells infected with the recombinant viruses and those infected with the parent DEV. Both recombinant viruses induced neutralizing antibodies against DHAV-3 and DEV in ducks. A single dose of the recombinant viruses induced solid protection against lethal DEV challenge and completely prevented DHAV-3 infection as early as 7 days post-vaccination. These recombinant P1- and 3C-expressing DEVs provide potential bivalent vaccines against DEV and DHAV-3 infection in ducks.

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