Genotyping of Giardia intestinalis from domestic and wild animals in Japan using glutamete dehydrogenase gene sequencing

Itagaki, Tadashi; Kinoshita, Shizuka; Aoki, Mikiko; Itoh, Naoyuki; Saeki, Hideharu; Sato, Naoto; Uetsuki, Junya; Izumiyama, Shinji; Yagita, Kenji; Endo, Tokiko

doi:10.1016/j.vetpar.2005.05.061

Cited by 93 publications

(50 citation statements)

References 23 publications

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“…These transmission cycles reflect the close association shared Page 14 of 31 A c c e p t e d M a n u s c r i p t 14 between dogs and humans, as well as the close interaction shared between dogs as pack animals in these Temple communities, which is expected in urban environments (Itagaki et al, 2005). There was however, an unusual dominance of Assemblage A genotypes of G. duodenalis in dogs as well as in humans in our study, supporting a number of previous studies (Traub et al, 2004;Eligio-Garcia et al, 2005;Itagaki et al, 2005;Lalle et al, 2005) indicating that Assembalge A may be most significant genotype when dealing with zoonotic potential. Dog-specific Assemblages C and D of G. duodenalis were also recovered from a moderate number of humans in this study.…”

Section: Discussionmentioning

confidence: 97%

“…This conclusion has largely been drawn from data showing that G. duodenalis is one of the most common enteric parasites of dogs in both developed as well as disadvantaged communities worldwide (Itoh et al, 2001;PonceMacotela et al, 2005) and that genetically identical, potentially zoonotic genotypes of G. duodenalis (predominantly Assemblage A) may exist in humans and dogs living within the same locality (Ponce-Macotela et al, 2002;Traub et al, 2004;Eligio-Garcia et al, 2005;Lalle et al, 2005) . On the other hand, non-zoonotic or dog-specific cycles of G. duodenalis transmission have also been shown to exist in dogs in communities where it is hypothesised that the frequency of transmission of Giardia among dogs is high (Hopkins et al, 1997;Itagaki et al, 2005;Palmer et al, 2008). In this study we determine the prevalence, genotypes and associated risk factors for G. duodenalis occurring in dogs and humans in Temples and their surrounding communities in Bangkok, Thailand.…”

Section: Introductionmentioning

confidence: 96%

“…Recent literature has focussed on the role of companion animals as providing the greatest risk of zoonotic transmission of G. duodenalis ( Traub et al, 2004;Eligio-Garcia et al, 2005;Itagaki et al, 2005;Lalle et al, 2005). This conclusion has largely been drawn from data showing that G. duodenalis is one of the most common enteric parasites of dogs in both developed as well as disadvantaged communities worldwide (Itoh et al, 2001;PonceMacotela et al, 2005) and that genetically identical, potentially zoonotic genotypes of G. duodenalis (predominantly Assemblage A) may exist in humans and dogs living within the same locality (Ponce-Macotela et al, 2002;Traub et al, 2004;Eligio-Garcia et al, 2005;Lalle et al, 2005) .…”

Section: Introductionmentioning

confidence: 99%

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Transmission cycles of Giardia duodenalis in dogs and humans in Temple communities in Bangkok—A critical evaluation of its prevalence using three diagnostic tests in the field in the absence of a gold standard

et al. 2009

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Page 1

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

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Transmission cycles of Giardia duodenalis in dogs and humans in Temple communities in Bangkok—A critical evaluation of its prevalence using three diagnostic tests in the field in the absence of a gold standard

et al. 2009

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“…(1) Thompson et al (2000), (2) Sogin et al (1989), (3) van Keulen et al (1995), (4) Not yet published, Xiao, S., South China Agricultural University, 2005, (5) Healey et al (1990), (6) , (7) Monis et al (1999), (8) (2000), (20) Yee and Dennis (1992), (21) Ey et al (1997), (22) Leonhard et al (2006), (23) Monis et al (1996), (24) Ey, P., University of Adelaide, 2002, submitted as a set with those from Ey et al (1997), (25) , (26) Itagaki et al (2005), (27) Matsubayashi et al (2005), (28) Robertson et al (2006), (29) Mowatt et al (1994), (30) Trout et al (2003), (31) Baruch et al (1996), (32) Sulaiman et al (2003), (33) Sulaiman et al (2004), (34) Not yet published, Mowatt, M., National…”

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confidence: 99%

Comparative evaluation ofGiardia duodenalissequence data

2007

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A review of the Giardia duodenalis sequences currently available on the GenBank database was completed to compare the different genotyping loci (small subunit ribosomal DNA, glutamate dehydrogenase, triose-phosphate isomerase and beta giardin) for their ability to discern assemblage and subassemblage groups and infer phylogenetic relationships. In total, 405 Giardia duodenalis sequences were sorted and aligned to examine the substitutions within and between the assemblages -A and B (zoonotic), C and D (dogs), E (livestock), F (cats) and G (rodents). It was found that all of the genes could reproducibly group isolates into their assemblages and that the AI/AII subassemblage groups were robust and identifiable at all loci. However, the assemblage B subgroups were not reproducible at half of the loci (small subunit ribosomal DNA and beta giardin), not due to their conserved nature, but because there was insufficient sequence data of reference isolates available for comparison. It is anticipated that further investigation of these loci may reveal the core subgroups of this medically important and zoonotic assemblage and also those of others. The closer, more recent, phylogenetic relationships amongst the assemblages appear to be resolved ; however, more sequence data from the current loci, and possibly new loci, will be required to establish the remaining relationships.

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“…isolates from humans have been shown exclusively to be of 2 major genotypes referred to as assemblages A and B, whereas assemblages C and D are more commonly found in dogs, and assemblage F is more commonly found in cats. 24 The objectives of this study were to determine the prevalence of Giardia spp. and Cryptosporidium spp.…”

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confidence: 99%

Comparison of Direct Immunofluorescence, Immunoassays, and Fecal Flotation for Detection of Cryptosporidium spp. and Giardia spp. in Naturally Exposed Cats in 4 Northern California Animal Shelters

Mekaru

Marks

Felley

et al. 2007

Veterinary Internal Medicne

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Background: Giardia spp. and Cryptosporidium spp. are common intestinal protozoan parasites in domestic cats. Few studies have critically evaluated the performance characteristics of commercially available immunoassays for detection of these organisms in the cat.Hypothesis: Human-based immunoassays are suboptimal for the detection of Giardia spp. and Cryptosporidium spp. in cats. Animals: Three-hundred-and-forty-four cats with diarrheic and nondiarrheic fecal specimens at 4 northern California animal shelters.Methods: A fecal specimen was collected from each cat in a case-controlled fashion. Fecal specimens were tested for Giardia spp. and Cryptosporidium spp. by using centrifugation flotation and 5 commercially available immunoassays (SNAP Giardia, ProSpecT Giardia Microplate Assay, ProSpecT Cryptosporidium Microplate Assay, ImmunoCard STAT! Cryptosporidium/ Giardia Rapid Assay, and Xpect Giardia/Cryptosporidium). Results were compared with a reference standard, the MeriFluor direct immunofluorescence assay.Results: Overall prevalences of Giardia spp. and Cryptosporidium spp. were 9.8 and 4.7%, respectively. The ProSpecT Microplate Assay had the highest sensitivities and specificities for Giardia spp. (91.2 and 99.4%) and Cryptosporidum spp. (71.4 and 96.7%), respectively. The SNAP Giardia antigen assay was easier to use and equally sensitive (85.3%) and specific (100%) to fecal flotation.Conclusions and Clinical Importance: Caution should be exercised when using human-based immunoassays for the diagnosis of Giardia and Cryptosporidium spp. in cats. Fecal flotation remains a useful method for detection of Giardia spp., can be used to detect other parasites, and has a sensitivity of 97.8% for detection of Giardia spp. when combined with the SNAP Giardia immunoassay.

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Genotyping of Giardia intestinalis from domestic and wild animals in Japan using glutamete dehydrogenase gene sequencing

Cited by 93 publications

References 23 publications

Transmission cycles of Giardia duodenalis in dogs and humans in Temple communities in Bangkok—A critical evaluation of its prevalence using three diagnostic tests in the field in the absence of a gold standard

Transmission cycles of Giardia duodenalis in dogs and humans in Temple communities in Bangkok—A critical evaluation of its prevalence using three diagnostic tests in the field in the absence of a gold standard

Comparative evaluation ofGiardia duodenalissequence data

Comparison of Direct Immunofluorescence, Immunoassays, and Fecal Flotation for Detection of Cryptosporidium spp. and Giardia spp. in Naturally Exposed Cats in 4 Northern California Animal Shelters

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