Genotyping of Giardia intestinalis Isolates in the Thrace Region, Turkey

Çiçek, Cemal; Şakru, Nermin

doi:10.5578/mb.10107

Cited by 11 publications

(4 citation statements)

References 19 publications

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“…Assemblage A has been more prevalently found than assemblage B (50‒80% vs 20‒30%) in children attending a hospital setting in Kocaeli Province (Tamer et al ., 2015) and in individuals of all ages in Aydin Province (Ertuğ et al ., 2016). Both assemblages were reported at similar proportions (~50%) in individuals admitted at hospital settings in Edirne (Çiçek and Şakru, 2015). Additionally, dogs and horses have been demonstrated to harbour infections by zoonotic sub-assemblage BIV and assemblage A, respectively (Gultekin et al ., 2017; Demircan et al ., 2019), whereas assemblages A and B were identified in river water samples in the Giresun and Samsun Provinces (Koloren et al ., 2016).…”

Section: Introductionmentioning

confidence: 78%

“…The molecular characterization of microscopy-positive G. duodenalis at three independent ( gdh , bg , tpi ) loci is perhaps the most relevant contribution of this study. This is, to the best of our knowledge, the first study adopting an MLG scheme to investigate the genetic diversity of G. duodenalis in Turkey, as previous surveys conducted in the country were based in single genetic locus, mainly bg (Çiçek and Şakru, 2015; Tamer et al ., 2015; Ertuğ et al ., 2016). MLG schemes significantly improve sensitivity for molecular analyses of virulence types, zoonotic potential and source tracking for G. duodenalis assemblages and sub-assemblages (Ankarklev et al ., 2018).…”

Section: Discussionmentioning

confidence: 95%

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Detection of enteric parasites and molecular characterization of Giardia duodenalis and Blastocystis sp. in patients admitted to hospital in Ankara, Turkey

et al. 2020

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This epidemiological study assesses the occurrence of enteric parasites in 4303 patients attended at two public hospitals in Ankara (Turkey) during 2018–2019. Microscopy was used as a screening test. Giardia duodenalis was also identified using a commercial ELISA for the detection of parasite-specific coproantigens. Giardia-positive samples by microscopy/ELISA were confirmed by real-time PCR and characterized using a multilocus genotyping scheme. Blastocystis sp. was genotyped in a sample subset. Blastocystis sp. (11.1%, 95% CI 11.4‒14.8%) and G. duodenalis (1.56%, 95% CI 1.22‒1.96) were the most prevalent pathogens found. Cryptosporidium spp., Entamoeba histolytica and intestinal helminths were only sporadically (<0.5%) found. For G. duodenalis, sequence (n = 30) analyses revealed the presence of sub-assemblages AII (23.3%), discordant AII/AIII (23.3%) and mixed AII + AIII (6.7%) within assemblage A, and BIII (10.0%), BIV (3.3%) and discordant BIII/BIV (23.3%) within assemblage B. Two additional sequences (6.7%) were assigned to the latter assemblage but sub-assemblage information was unknown. No associations between G. duodenalis assemblages/sub-assemblages and sociodemographic and clinical variables could be demonstrated. For Blastocystis sp., sequence (n = 6) analyses identified subtypes ST1, ST2 and ST3 at equal proportions. This is the first molecular characterization of G. duodenalis based on MLG conducted in Turkey to date.

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Section: Introductionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 95%

Detection of enteric parasites and molecular characterization of Giardia duodenalis and Blastocystis sp. in patients admitted to hospital in Ankara, Turkey

et al. 2020

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“…intestinalis genotypes vary according to the regions where the study was conducted. While assemblage A is more common in the USA, Mexico, Brazil, Portugal, and Italy, Assemblage B is dominant in England, France, Netherlands, Norway, Nigeria, Argentina, Egypt, and Bangladesh ( 18 , 19 ). In Türkiye, assemblages A and B were detected in studies conducted in Ankara ( 20 ), Sivas ( 21 ), Aydın ( 17 ), and Manisa ( 22 ), and assemblage A was the dominant genotype.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Giardia intestinalis and Cryptosporidium spp. Detected in Humans in Ağrı, Türkiye

Aydemir,

Barlık,

Ekici

et al. 2024

IJPA

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Background: We aimed to determine the prevalence of Giardia intestinalis and Cryptosporidium spp. in patients who admitted hospital with diarrhea and to gain information about the transmission of these parasites in Ağrı, Türkiye. Methods: This study included 184 patients who applied to Ağrı-Diyadin State Hospital, Türkiye in 2022. The immunochromatographic card test was used for detection of the G. intestinalis and Cryptosporidium spp. Nested PCR-RFLP analysis of the COWP gene and sequence analysis of the gp60 gene were used to genotype and subtype Cryptosporidium spp., whereas Nested PCR and sequence analyses of β-giardin gene were used genotype G. intestinalis. Results: Of the 184 stool specimens examined, 12 (14.29%) and 7 (3.80%) were positive for G. intestinalis and Cryptosporidium spp., respectively. The Cryptosporidium species were identified as C. parvum belonging to the IId subtype family. The G. intestinalis were identified assemblages A. Conclusion: Assemblage A, which is associated with diarrhea, is responsible for giardiasis and C. parvum IId subtype, often found in sheep, goats and cattle, is responsible for cryptosporidiosis in Ağrı, Türkiye.

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“…G. duodenalis infections in humans have been molecularly identified in at least 55 countries, with the molecular prevalence ranging from 0.03% (9/26886) in Xinjiang, China [ 18 ] to 82.05% (32/39) in Turkey [ 19 ]. The pooled molecular prevalence of G. duodenalis in humans is at 9.72% (10,921/112383), with infection rates ranging from 0.40% (210/51924) in China to 62.22% (28/45) in Tanzania.…”

Section: Molecular Epidemiology Of G Duodenalis In...mentioning

confidence: 99%

Assessment of potential zoonotic transmission of Giardia duodenalis from dogs and cats

Sun,

Qin,

et al. 2023

One Health

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Genotyping of Giardia intestinalis Isolates in the Thrace Region, Turkey

Cited by 11 publications

References 19 publications

Detection of enteric parasites and molecular characterization of Giardia duodenalis and Blastocystis sp. in patients admitted to hospital in Ankara, Turkey

Detection of enteric parasites and molecular characterization of Giardia duodenalis and Blastocystis sp. in patients admitted to hospital in Ankara, Turkey

Molecular Characterization of Giardia intestinalis and Cryptosporidium spp. Detected in Humans in Ağrı, Türkiye

Assessment of potential zoonotic transmission of Giardia duodenalis from dogs and cats

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