Genotyping of Haemophilus influenzae type b in pre-vaccination era

Multilocus sequence typing and ftsI sequencing: a powerful tool for surveillance of penicillin-binding protein 3-mediated beta-lactam resistance in nontypeable Haemophilus influenzae

“…We are aware of a number of previous studies where MLST and ftsI sequencing was performed [3,4,12,23-25,43-45]. To our knowledge, four reports have linked MLST data and PBP3 substitution patterns: one presented the allelic profiles of 83 group III respiratory isolates from Japan [43]; another presented the substitution pattern of a single group II ST368 NTHi isolate causing meningitis in Italy [44]; and two most recent publications presented the substitution patterns and STs of 95 respiratory [25] and 18 invasive isolates [24] from Spain.…”

Individual Risk Factors Associated With Nasopharyngeal Colonization With Streptococcus pneumoniae and Haemophilus influenzae

“…according to eligibility criteria, we prospectively monitored 349 children (participation rate: 98.3%) as healthy subjects. 9 inclusion and exclusion criteria are described in detail elsewhere. 9…”

“…in addition, due to limited movement of people (particularly children) between sado island and the Japanese mainland, the island can be considered as an appropriate environment for investigating antimicrobial usage and resistant pathogens. [7][8][9] in this prospective birth cohort study, a total of 467 live births where recorded on sado island during the study period. according to eligibility criteria, we prospectively monitored 349 children (participation rate: 98.3%) as healthy subjects.…”

“…5,6 Our objectives were to (1) identify factors associated with the carriage of nasopharyngeal pathogens and (2) evaluate the serotype distributions of S. pneumoniae and H. influenzae under the introductory era of PCV7 and Hib vaccine in sado island, Japan, an area with low antimicrobial pressure. [7][8][9] MATERIALS AND METHODS Subject this study was conducted from January 2008 to december 2011 as a birth cohort study in sado island and officially named, the "sado island, antimicrobials, day care attendance, Older siblings (sadO)-study." 9 there are only 5 pediatricians in the island working at 2 hospitals.…”

Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus Isolated from Children in a Community with Low Antimicrobial Pressure in Japan

“…In addition, MRSA isolates were collected from nasal mucosa samples obtained from healthy children aged 1 month, 4 months, 7 months, 10 months, 18 months, and 3 years at health checkups between March 2008 and January 2012 as part of the surveillance program on Sado Island (10,11,13). Any healthy subject identified with MRSA was excluded from the next screening.…”