Genotyping of<i>Streptococcus uberis</i>isolates in healing process of bovine clinical mastitis

Werner, Christina; Sauerwald, Claudia; Sundrum, Albert; El-Sayed, Amr; Zschöck, M.

doi:10.1016/j.ijvsm.2018.09.005

Cited by 5 publications

(6 citation statements)

References 19 publications

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“…This inherently high genetic diversity renders the attribution of a specific set of virulence factors (and associated pathogenesis) to a given S. uberis strain/s challenging 12 , 22 , 23 . The development of strategies to control this important mastitis-causing organism necessitates monitoring of the genetic diversity and determination of the significance of any detected diversity to the disease pathogenesis 22 , 24 . Therefore, the main objectives of this study were to compare S. uberis isolates cultured from dairy cows with clinical mastitis in distinctly different geographic and climatic regions of Australia.…”

Section: Introductionmentioning

confidence: 99%

Sequence characterisation and novel insights into bovine mastitis-associated Streptococcus uberis in dairy herds

Vezina

Al-harbi

Ramay

et al. 2021

Sci Rep

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Streptococcus uberis is one of the most frequent mastitis-causing pathogens isolated from dairy cows. Further understanding of S. uberis genetics may help elucidate the disease pathogenesis. We compared the genomes of S. uberis isolates cultured from dairy cows located in distinctly different geographic regions of Australia. All isolates had novel multi locus sequence types (MLST) indicating a highly diverse population of S. uberis. Global clonal complexes (GCC) were more conserved. GCC ST86 and GCC ST143 represented 30% of the total isolates (n = 27) and were clustered within different geographic regions. Core genome phylogeny revealed low phylogenetic clustering by region, isolation source, and MLST. Identification of putative sortase (srtA) substrates and generation of a custom putative virulence factor database revealed genes which may explain the affinity of S. uberis for mammary tissue, evasion of antimicrobial efforts and disease pathogenesis. Of 27 isolates, four contained antibiotic resistance genes including an antimicrobial resistance cluster containing mel/mef(A), mrsE, vatD, lnuD, and transposon-mediated lnuC was also identified. These are novel genes for S. uberis, which suggests interspecies lateral gene transfer. The presence of resistance genes across the two geographic regions tested within one country supports the need for a careful, tailored, implementation and monitoring of antimicrobial stewardship.

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Section: Introductionmentioning

confidence: 99%

Sequence characterisation and novel insights into bovine mastitis-associated Streptococcus uberis in dairy herds

Vezina

Al-harbi

Ramay

et al. 2021

Sci Rep

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“…We defined this strain also as persistent due to PFGE fingerprinting. In contrast to Werner et al (2018), the in-herd similarity of Strep. uberis strains was higher than the similarity from different herds (Figure 1).…”

Section: Discussionmentioning

confidence: 79%

“…In our study, 2 important findings underline the high prevalence, strain diversity, and global spread of environment-associated Strep. uberis (Werner et al, 2018;Käppeli et al, 2019). First, in a small number of dairy cows, the udder was colonized by up to 3 unrelated genotypes during the same sampling event.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of the population structure of Streptococcus uberis mastitis isolates from Austrian small-scale dairy farms and a Slovakian large-scale farm

Wald

Baumgartner

Gutschireiter

et al. 2020

Journal of Dairy Science

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Streptococcus uberis, a major mastitis pathogen associated with intramammary infections (IMI), can be found ubiquitously in the cow's environment. Although Strep. uberis is reported to be susceptible to most antimicrobials, in practice poor responses to treatment and recurrent mastitis are observed. This can be explained by reinfection or by persistence of strains. We hypothesized that among a heterogeneous group of Strep. uberis mastitis isolates, some predominant host-adapted clones might be recurrently isolated from IMI. Therefore, the aim of this pilot study was to determine the Strep. uberis genotype variety found among small-scale dairy herds (127 Austrian dairy farms) and compare this with a large-scale herd (a Slovakian dairy farm). We determined the occurrence and strain diversity of Strep. uberis (n = 309) isolates using molecular analysis. Streptococcus uberis isolates from aseptically collected quarter milk samples were genotypically characterized using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing. The Strep. uberis strain set covered isolates from 4 Austrian federal areas [

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“…They cause inflammation that blocks the ducts and leads to a decrease in milk production, an increase in the number of somatic cells, and ultimately to the involution of the alveolar apparatus. In addition, there are several enzymes/toxins in the capacitive system of the udder that are very sensitive to antibiotics (Werner et al, 2018). Staphylococcus aureus (St. aureus) is the main cause of mastitis.…”

Section: Elevated Levels Of Somatic Cell Levels May Indicate Parasecrmentioning

confidence: 99%

Species composition of microbiota of cows udder and raw milk quality at mastitis

Palii¹,

Ulko²,

Bogomolov³

et al. 2020

Ukr J Ecol

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To increase the productivity of cows and improve the nutritional and sanitary-technological qualities of milk is one of the most important tasks in the development of dairy farming in all countries of the world. The cause of degradation of these parameters is such a widespread disease of cows as mastitis. The social significance of mastitis is manifested in the fact that the causative agents of mastitis in cows can cause disease in humans. Since streptococci and staphylococci play a leading role in mastitis, they are the most common in milk. Studies have shown that udder disease in cows with mastitis at the beginning of lactation has a certain seasonal nature and its main peaks occur in March-May and September-November. During these months, from 2.5% to 4.1% of cows from the total number of livestock of the farm were found to have mastitis. Physiochemical features of milk from the cows with mastitis were an increase in the number of somatic cells to 1,500,000/cm3, increase of milk pH to 7.3, caused by the breakdown of milk proteins into ammonia, the increase in milk electrical conductivity to 5.92-7.54 mS/cm, associated with the entry of sodium and chlorine ions from the blood into the milk. In studies of cows with mastitis, 16 species of bacteria were isolated from the secretion of the udder. In monoculture, the microflora, that was found in 30.5% of cows, consisted of E. coli, St. epidermidis, C. freundi, Sh. Dysentery, St. aureus, St. hyicus spp. Chromogenes, Str. Agalactiae, St. lentus, and St. intermediate. In 69.5% of cows with mastitis, the microflora was found in various associations. The most common associations of bacteria were St. epidermidis + St. aureus + Str. agalactiae + Str. haemolyticus; E. coli + Str. agalactiae; Str. agalactiae + St. epidermidis; St. epidermidis + St. aureus + Str. agalactiae. We developed a method for diagnosing mastitis in cows, which was to diagnose the disease at early stage of inflammation of the mammary glands by studying the composition of the milk the examined lobe of the udder.

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Genotyping ofStreptococcus uberisisolates in healing process of bovine clinical mastitis

Cited by 5 publications

References 19 publications

Sequence characterisation and novel insights into bovine mastitis-associated Streptococcus uberis in dairy herds

Sequence characterisation and novel insights into bovine mastitis-associated Streptococcus uberis in dairy herds

Comparison of the population structure of Streptococcus uberis mastitis isolates from Austrian small-scale dairy farms and a Slovakian large-scale farm

Species composition of microbiota of cows udder and raw milk quality at mastitis

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