Genotyping of the kappa‐casein and beta‐lactoglobulin genes in Anatolian water buffalo by <scp>PCR</scp>‐<scp>RFLP</scp>

Çınar, Mehmet Ulaş; Akyüz, Bilal; Arslan, Korhan; Ilgar, Esma Gamze

doi:10.1111/1471-0307.12257

Cited by 6 publications

(6 citation statements)

References 16 publications

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“…Those fragments are termed as allels. This finding is different from cattle which has polymorphic for the kappa casein gene (Doosti et al 2011;Khaizaran & Al-Razem 2014;Cinar et al 2016). This finding of genotype BB in all buffalo samples (swamp and river) in Indonesia is similar to previous reports of allel frequencies which found in most of buffalo (Table 2).…”

Section: Genotyping Of Kcn Genesupporting

confidence: 78%

Genotyping of Kappa-Casein Gene of Buffalo in Indonesian

Margawati

Volkandari²,

Indriawati³

et al. 2017

Proceedings of International Seminar on Livestock Production and Veterinary Technology

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Casein (KCN) is a milk protein with four variants of alpha S1, alpha S2, beta and kappa which has different allel of each. Kappa Casein (CSN3) gene lies at exon 4 associating with fat and protein milk contens. Genetic variation of CSN3 gene influences quality and composition of milk. The aim of this research was to genotype the CSN3 gene of Bufalos from several places in Indonesia. Amount of 29 buffalo samples was used in this research, those consisted of 15 collected from Medan, 3 samples of Banyuwangi, 10 samples of Baluran and one sample of South-East Nusa Tenggara (NTT). A Polymerase Chain Reaction-Restiction Fragment Length Polymorphism (PCR-RFLP) method was used for CSN3 gene analysis. The CSN3 gene was amplified at 56°C of annealing with 30 cycles and resulted a right fragment of 379 bp. The fragments were digested with HindIII at 37°C during for four hours for genotyping analysis. The result showed that BB genotypes were found at the locus of CSN3 gene in all samples. Those BB genotypes merged the pattern of digested fragments with sizes of 255 and 154 bp. This finding suggests that genotyping using HindIII reveals monomorphics of BB types accociated with milk content of casein.

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Section: Genotyping Of Kcn Genesupporting

confidence: 78%

Genotyping of Kappa-Casein Gene of Buffalo in Indonesian

Margawati

Volkandari²,

Indriawati³

et al. 2017

Proceedings of International Seminar on Livestock Production and Veterinary Technology

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“…PCR-RFLP results using AcuI enzyme disagreed with the majority of reviewed PCR-RFLP studies conducted by HindIII , HinfI or MaeII restriction enzymes which concluded that, all Egyptian buffaloes were monomorphic and genotyped as homozygous "BB" animals as reported by Othman (2005); El-Rafey and Darwish (2008); and Abdel Dayem et al (2009). Similar results were also noticed, in Pakistani buffalo (Riaz et al, 2008;Ghafoor et al, 2015), in Indian buffalo (Gangaraj et al, 2008;Shende et al, 2009;Nair et al, 2011;George et al, 2018), in Brazilian buffalo (Otaviano et al, 2005), in Chinese water buffalo (Ren et al, 2011) , in Iraqi buffalo (Jaayid et al, 2013), in Anatolian water buffalo breed (Cinar et al, 2016), in swamp and river Indonesian buffaloes (Margawati et al, 2016), in Cuban buffalo (Marrero et al, 2016), and in Iranian buffalo (Abbasi et al, 2009).…”

Section: Detection Of Polymorphism and Genetic Variants Of κ-Cn And β-Lgsupporting

confidence: 65%

“…Also, the dominance of "BB" genotype (0.66) was reported by Karimi et al (2009). On contrary, Patel et al (2007) and Cinar et al (2016) noticed the predominance of heterozygous "AB" with genotypic frequencies of 0.586 and 0.698, respectively.…”

Section: Allelic and Genotypic Frequencies Of Studied Genesmentioning

confidence: 92%

“…As well as, some other investigations conducted on different buffalo breeds found two alleles "A" and "B" for β-LG gene, some of them recorded the predominance of "B" allele with values of 0.595 and 0.8125 as reported by Patel et al (2007) and Karimi et al (2009), respectively. From the other hand, Riaz (2013) and Cinar et al (2016) found that "A" allele was the most frequent allele in Nili-Ravi and Anatolian water buffaloes with values of 0.64 and 0.603 respectively. Whereas, Nair et al (2011) detected only the heterozygous genotype "AB" (100%) and the allelic frequency of 0.5 for each allele; "A" and "B" of β-LG in all Nagpuri buffaloes of India.…”

Section: Allelic and Genotypic Frequencies Of Studied Genesmentioning

confidence: 99%

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Polymorphism, Allelic and Genotypic Frequencies of κ-Casein and β-LG genes in Egyptian Buffaloes

Al-Shawa¹,

El-Zarei²,

Ghazy³

et al. 2020

JAS

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With a view to detecting the genotypes of both κ-CN and β-LG genes in native populations of Egyptian buffalo using PCR-RFLP technique, 80 randomly, individuals were selected from five geographical locations of some Egyptian provinces. Also, to estimate the population genetic parameters such as, allelic and genotypic frequencies, heterozygosity, and inbreeding coefficient (F IS ) of these studied genes. For genotyping, 453 bp PCR product of κ-CN was digested with AcuI and HpyCH4IV (Isoschizomer for MaeII) restriction enzymes while the 247 bp PCR product of β-LG was digested with HaeIII restriction enzyme. PCR-RFLP results discovered polymorphism at the level of κ-CN gene in all studied Egyptian buffaloes with two distinct alleles "A" and "B". PCR-RFLP analysis for κ-CN gene using both restriction enzymes successfully detected that polymorphic status of the studied populations. We recommended using AcuI enzyme which was more capable for differentiating between homozygous (17%) and heterozygous (83%) individuals than HpyCH4IV enzyme which defined only 4% of homozygous individuals and the remaining was heterozygous (96%) individuals. Existence of heterozygosity excess in all studied populations referred to higher degree of genetic variability between individuals within these populations. On contrary, results of PCR-RFLP at the level of β-LG gene revealed a monomorphic pattern of Egyptian buffaloes and genotyped as "AA" animals which signified that PCR-RFLP assay with HaeIII enzyme for β-LG gene failed to discover any evidence of polymorphism in Egyptian buffalo under the circumstances of this study or all studied animals possess only one allele.

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“…A polymorphism in Pit1 gene was associated with milk traits in Egyptian and Indian buffaloes (Othman et al, 2011;Parikh and Rank, 2013). In addition, water buffaloes carry polymorphism in the KCN gene (Cinar et al, 2015;Mitra et al, 1998), which can affect their milk yield as well as feta cheese processing parameters (Shakerian et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Effects of genetic polymorphism in Pit1, GH, GHR and KCN3 on milk yield and body weight of Khuzestan (Iran) water buffaloes

Ahmadzadeh

Rashidi

Najafabadi

et al. 2019

Rev Colom Cienc Pecua

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Background: Genetic information is necessary to devise strategic plans aimed to improve the genetic merit of buffalos. Objective: To assess the effect of genetic polymorphisms in GH, Pit-1, GHR, GHRHR, and KCN3 genes on milk production and body weight of Khuzestan water buffaloes. Methods: Blood samples were collected from 60 buffaloes from the Khuzestan province, Iran. Using the PCR-RFLP technique, the amplified and digested fragments of GH/AluI, GHR/AluI, GHRHR/ HaeIII, Pit1/HinfI, and KCN3/HindIII were genotyped. Results: All animals were monomorphic for GHRHR. The frequency of mutant alleles for GH, GHR, KCN3, and Pit1 was 47.5, 74.2, 49.2, and 51.7%, respectively. There were significant differences (p<0.0001) in the genotypic frequencies of GH, GHR, and Pit1 between high and low milk-yielding buffaloes. The GH (p=0.0002), GHR (p<0.0001) and Pit1 (p<0.0001) polymorphisms also had significant effects on body weight. Sequencing results revealed the presence of C496A, G495A, G498A and C1501T SNPs in the GH, and G1702T in the GHR gene of Khuzestan buffalos. Conclusion: This study highlights the importance of GH, GHR, and Pit1 on milk production and body weight of Khuzestan buffaloes. The results suggest that devising an integrated breeding plan in Khuzestan water buffalos can considerably benefit from the very high diversity in candidate genes.Keywords: animal breeding, casein, genetic variation, growth hormone, milk production, SNP. ResumenAntecedentes: La información genética es necesaria para diseñar planes estratégicos con el objeto de mejorar el mérito genético de los búfalos. Objetivo: Evaluar el efecto de los polimorfismos genéticos en los genes GH, Pit-1, GHR, GHRHR y KCN3 sobre la producción láctea y peso corporal de búfalos de agua de la provincia de Juzestán, Iran. Métodos: Se recolectaron 60 muestras de sangre de búfalos de la provincia de Juzestán, en Irán. Los fragmentos amplificados y digeridos de GH/AluI, GHR/AluI, GHRHR/HaeIII, Pit1/HinfI y KCN3/HindIII fueron clasificados genotípicamente, utilizando la técnica PCR-RFLP. Resultados: Todos los animales fueron monomórficos para el gen GHRHR. La frecuencia alélica de alelos mutantes para los genes GH, GHR, KCN3 y Pit1 fue 47,5, 74,2, 49,2 y 51,7%, respectivamente. Se encontraron diferencias significativas (p<0,0001) en las frecuencias genotípicas de GH, GHR y Pit1 entre búfalos de alta y baja producción. El efecto del polimorfismo GH (p=0,0002), GHR (p<0,0001) y Pit1 (p<0,0001) también fue significativo para peso corporal. Los resultados de la secuenciación revelaron la presencia de SNPs C496A, G495A, G498A y C1501T en GH, y G1702T en el gen GHR. Conclusiones: Este estudio resalta la importancia de los genes GH, GHR y Pit1 sobre la producción de leche y el peso corporal de búfalos de Juzestán. Los resultados sugieren que la elaboración de un plan de cruzamiento integrado en búfalos de agua de Juzestán puede beneficiarse considerablemente de la gran diversidad de genes candidatos.Palabras clave: caseína, hormona del crecimiento, producción de leche, reproducción animal, SNP, variación genética. ResumoAntecedentes: Determinação informações genéticas é o passo crítico para elaborar planos estratégicos com o objetivo de melhorar o mérito genético dos búfalos. Objetivo: Avaliar o efeito de polimorfismos genéticos nos genes GH, Pit-1, GHR, GHRHR e KCN3 na produção de leite e no peso corporal dos búfalos de água do Cuzistão, Irã. Métodos: Amostras de sangue foram coletadas de 60 búfalos da província de Cuzistão, no Irã. Utilizando a técnica PCR-RFLP, os fragmentos amplificados e digeridos de GH/AluI, GHR/AluI, GHRHR/HaeIII, Pit1/HinfI e KCN3/HindIII foram genotipados. Resultados: Todos os animais eram monomórficos para o gene GHRHR. A freqüência alélica de alelos mutantes para os genes GH, GHR, KCN3 e Pit1 foi 47,5, 74,2, 49,2 e 51,7%, respectivamente. Uma diferença significativa (p<0,0001) foi encontrada nas freqüências genotípicas de os genes GH, GHR e Pit1 entre búfalos de alta e baixa produção. O efeito do polimorfismo GH (p=0,0002), GHR (p<0,0001) e Pit1 (p<0,0001) também foi significativo para o peso corporal. Os resultados da sequenciação revelaram a presença de SNPs C496A, G495A, G498A e C1501T no GH, e G1702T no gene GHR dos buffalos do Cuzistão. Conclusões: Este estudo destacou a importância da GH, GHR e Pit1 na produção de leite e no peso corporal de buffalos do Cuzistão. Os resultados sugerem que a elaboração de um plano de melhoramiento genético integrado em búfalos de água do Cuzistão pode beneficiar consideravelmente da grande diversidade de genes candidatos.Palavras-chave: caseína, hormônio do crescimento, produção de leite, reprodução animal, SNP, variação genética.

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Genotyping of the kappa‐casein and beta‐lactoglobulin genes in Anatolian water buffalo by PCR‐RFLP

Cited by 6 publications

References 16 publications

Genotyping of Kappa-Casein Gene of Buffalo in Indonesian

Genotyping of Kappa-Casein Gene of Buffalo in Indonesian

Polymorphism, Allelic and Genotypic Frequencies of κ-Casein and β-LG genes in Egyptian Buffaloes

Effects of genetic polymorphism in Pit1, GH, GHR and KCN3 on milk yield and body weight of Khuzestan (Iran) water buffaloes

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