Genotyping pattern of the vacuolating cytotoxin A and cytotoxin associated gene A of the Helicobacter pylori strains detected in fecal samples of household dogs

Abstract

In despite of the high clinical impact of Helicobacter pylori, its exact sources and routes of transmission are unknown. Dogs may play an imperative role in the transmission of H. pylori to humans. The current investigation was done to study the status of vacA and cagA genotypes in the H. pylori strains of dogs. One-hundred and fifty fecal samples were collected from healthy and complicated household dogs. Genomic DNA was extracted from fecal samples and presence of 16S rRNA gene was studied using the PCR amplification. Distribution of vacA and cagA genotypes were studied by the multiplex PCR.

Dog as Potential Source of Helicobacter Pylori in Egypt: Public Health Significance

“…Low levels of hygiene used for maintenance of dogs, their close contact with stray animals could be a risk factor in increasing the prevalence of Helicobacter. Moreover, a research was done by [35] in household dogs raised under hygienic conditions and fed with cooked food revealed a low prevalence (8.66%). Although there is a signi cant presence of helicobacteria in dogs, it is not possible to relate it with gastric alterations in these animals [36][37][38].…”