Gentamicin- and Methicillin-Resistant &lt;i&gt;Staphylococcus aureus&lt;/i&gt;: Phenotypic and Genotypic Characterization of Three Putative Nosocomial Outbreak Strains

Traub, Walter H.; Leonhard, Birgit; Bauer, Dierk

doi:10.1159/000239419

Cited by 6 publications

(7 citation statements)

References 26 publications

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“…Nevertheless, these preparations might neu tralize various exoproteins and exotoxins of S. aureus. Unfortunately, we were not able to examine the rabbit and patient sera for anti bodies against enterotoxin A, as all isolates comprising MRSA outbreak strain I were shown to produce this enterotoxin [7], but not toxic shock syndrome toxin-1 [50],…”

Section: Discussionmentioning

confidence: 99%

“…1,2,6,31,43. and 44. All isolates comprised MRSA outbreak strain I and had been identified as previously described [7], The S. aureus strain Lafferty was a gift from Dr. Joseph Wheat, Department of Medicine, Wishard Me morial Hospital, Indianapolis, Ind., USA. The S. au reus strain ATCC 25923 and the Bacillussubtilis strain ATCC 6633 were employed for control and antibiotic detection purposes.…”

Section: Bacteriamentioning

confidence: 99%

“…The S. au reus strain ATCC 25923 and the Bacillussubtilis strain ATCC 6633 were employed for control and antibiotic detection purposes. The bacteria were maintained as described before [7],…”

Section: Bacteriamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Commencing in 1982/1983, a strain of MRSA appeared at our institution [4] which persisted into the 1990s [5][6][7]. This strain, designated strain I [7], caused many episodes of systemic infection, including complicated septicemias. During our investigations re garding this strain, it was of interest to deter mine whether serological tests might help in differentiating between colonization and sig nificant infection of patients by MRSA strain I.…”

Section: Introductionmentioning

confidence: 99%

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Immunobiology of Methicillin-Resistant <i>Staphylococcus aureus</i>: Immune Response of Rabbits and Patients to Systemic Infection

Traub

Bauer²,

Leonhard³

1996

Chemotherapy

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Teichoic acid (TA) and peptidoglycan (PG) extracted from Staphylococcus aureus strains ATCC 25923 and Lafferty as well as formalinized cells of these two strains and several clinical methicillin-resistant S. aureus (MRSA) isolates were immunogenic for New Zealand White rabbits. Rabbits which had recovered from experimental bacteremia due to MRSA seroconverted, i.e. demonstrated raised titers of antibodies against TA and PG of the S. aureus strain Lafferty and against whole cells (WC) and ultrasound cell lysates (UCL) of MRSA isolates No. 1 and 2 (representative of nosocomial MRSA strain I), as determind with enzyme-linked immunosorbent assays. Furthermore, sera from 2 long-term survivor rabbits recognized four polypeptides (apparent molecular weights = 38.9, 33.9, 30.9, and 28.2 kDa) shared by UCL extracts from MRSA isolates No. 1 and 2, as determined with immunoblots. Neither normal nor immune rabbit sera augmented the bactericidal activity of fresh defibrinated human blood (65% v/v) against selected MRSA isolates and S. aureus strain ATCC 25923. Sera from 12 patients with documented systemic infection due to MRSA outbreak strain I were examined for IgM and IgG antibodies against TA, WC, and UCL antigens. Three patient sera exhibited raised IgM antibodies against TA; 7 of 12 patient sera showed increased IgG anti-TA titers. Only 1 patient had a markedly raised IgM anti-WC titer, whereas 4 and 10 of the patients had increased IgG titers against WC from MRSA isolates No. 1 and 2, respectively. However, all 12 patients had raised IgG titers against UCL from MRSA isolate No. 2 versus 4 of 12 patients with elevated IgG titers against UCL from MRSA isolate No. 1. Immunoblots with 3 selected patient sera revealed IgG antibodies to be more multifaceted than IgM antibodies. Sera from 11 of the 12 patients contained antimicrobial drug(s); yet only 5 of these 11 sera (used at 10% v/v in broth) killed inocula of MRSA isolate No. 43. None of the 12 patient sera (10% v/v) enhanced the bactericidal activity of human blood against selected MRSA isolates. Neither three commercial intravenously applicable IgG preparations nor an IgG anti-α-hemolysin formulation (employed at 10% v/v) augmented the bactericidal activity of fresh defibrinated human blood against selected MRSA isolates comprising MRSA outbreak strain I.

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Section: Discussionmentioning

confidence: 99%

Section: Bacteriamentioning

confidence: 99%

Section: Bacteriamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Immunobiology of Methicillin-Resistant <i>Staphylococcus aureus</i>: Immune Response of Rabbits and Patients to Systemic Infection

Traub

Bauer²,

Leonhard³

1996

Chemotherapy

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Enhanced Efficacy of Superparamagnetic Iron Oxide Nanoparticles Against Antibiotic‐Resistant Biofilms in the Presence of Metabolites

et al. 2013

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Antibiotic resistance and the lack of new antibacterial agents cause major challenges for the treatment of infections. Here, we describe a simple, broad-spectrum, and low-cost dual-sided approach which uses superparamagnetic iron oxide particles (SPION) in combination with fructose metabolites as an alternative to existing antibacterial strategies. This strategy offers further improved efficacy of SPION against persistent gram-positive and gram-negative bacteria infections by manipulating the biofilm metabolic microenvironment and outperforms vancomycin (the antibiotic of last resort), creating a new nanotechnology-driven approach.

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Incidence and antimicrobial susceptibility of Staphylococcus aureus isolated from ready-to-eat foods of animal origin from tourist destinations of North-western Himalayas, Himachal Pradesh, India

et al. 2019

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This study was aimed to determine the incidence of Staphylococcus aureus in ready-to-eat (RTE) milk (n = 120) and meat (n = 120) products from various tourist places in north western Himalayas, Himachal Pradesh, India. S. aureus isolates and its enterotoxins; A, B, D and E were characterized by conventional and molecular methods. Antimicrobial susceptibility (AMS) profiles of S. aureus isolates were determined by disk diffusion method using Clinical and Laboratory Standards Institute criteria. Overall, 6.7% (n = 16/240) food samples were positive for S. aureus. PCR amplification of nucA confirmed all biochemically characterized isolates as S. aureus. Incidence of S. aureus was higher (10.0%) in RTE milk products than meat products (3.3%). S. aureus contamination levels were highest in milk cake/khoa (26.0%, p = 0.0002) followed by ice cream/kulfi (10.0%, p = 0.4), mutton momo (10.0%, p = 0.4), burfi (3.3%, p = 0.7) and chicken momo (3.3%, p = 0.7). None of the isolates carried genes for S. aureus enterotoxins; A, B, D and E. AMS testing revealed seven different resistance patterns and 81.3% multi drug resistance. All the isolates were resistant to ampicillin. High resistance levels were observed against methicillin (93.7%), clindamycin (68.8%), erythromycin (56.3%) and vancomycin (43.8%). Vancomycin resistant (n = 7) isolates were also resistant to methicillin. All isolates were susceptible to novobiocin.

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Gentamicin- and Methicillin-Resistant <i>Staphylococcus aureus</i>: Phenotypic and Genotypic Characterization of Three Putative Nosocomial Outbreak Strains

Cited by 6 publications

References 26 publications

Immunobiology of Methicillin-Resistant <i>Staphylococcus aureus</i>: Immune Response of Rabbits and Patients to Systemic Infection

Immunobiology of Methicillin-Resistant <i>Staphylococcus aureus</i>: Immune Response of Rabbits and Patients to Systemic Infection

Enhanced Efficacy of Superparamagnetic Iron Oxide Nanoparticles Against Antibiotic‐Resistant Biofilms in the Presence of Metabolites

Incidence and antimicrobial susceptibility of Staphylococcus aureus isolated from ready-to-eat foods of animal origin from tourist destinations of North-western Himalayas, Himachal Pradesh, India

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