Gentamicin coating of plasma chemical oxidized titanium alloy prevents implant-related osteomyelitis in rats

Diefenbeck, Michael; Schrader, Christian; Gras, Florian; Mückley, Thomas; Schmidt, Jürgen; Zankovych, S.; Bossert, Jörg; Jandt, Klaus D.; Völpel, Andrea; Sigusch, Bernd W.; Schubert, Harald; Bischoff, Sabine; Pfister, W.; Edel, Birgit; Faucon, Mathilde; Finger, Ulrich

doi:10.1016/j.biomaterials.2016.05.039

Cited by 80 publications

(42 citation statements)

References 28 publications

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“…For mouse and rat model, 10 5 colony-forming unit (CFU) bacterial suspension is often injected into the tibia bone marrow and fixed with different material steel pins [64, 118–120] Rat tibia implantation with bacterial infection[121] can be initiated by inoculation of a bacterial suspension at the site of implantation [122] or the use of a pre-colonized implant1. Mixed-models, involving the use of both a pre-colonized implant and a bacterial suspension [123], were reported to reproduce the clinical use of an infected implant and contaminated washing solutions at surgery, respectively.…”

Section: Evidence Of the Anti-infective Prophylactic Therapy Of Coatingmentioning

confidence: 99%

“…In mouse osteomyelitis model, there was no detectable bacterial presence 3 weeks after inoculation with S. aureus without burst releasing [107]. Gentamicin coating on titanium implants with gentamicin-sodiumdodecylsulfate (SDS) and tannic acid demonstrated a high preventive effect on IRIs, which showed successful rapid osseointegration [64]. The rapid osseointegration is necessary which meant repairing completed.…”

Section: Evidence Of the Anti-infective Prophylactic Therapy Of Coatingmentioning

confidence: 99%

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Coatings as the useful drug delivery system for the prevention of implant-related infections

Pan

Zhou

2018

J Orthop Surg Res

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Implant-related infections (IRIs) which led to a large amount of medical expenditure were caused by bacteria and fungi that involve the implants in the operation or in ward. Traditional treatments of IRIs were comprised of repeated radical debridement, replacement of internal fixators, and intravenous antibiotics. It needed a long time and numbers of surgeries to cure, which meant a catastrophe to patients. So how to prevent it was more important than to cure it. As an excellent local release system, coating is a good idea by its local drug infusion and barrier effect on resisting biofilms which were the main cause of IRIs. So in this review, materials used for coatings and evidences of prevention were elaborated.

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Section: Evidence Of the Anti-infective Prophylactic Therapy Of Coatingmentioning

confidence: 99%

Section: Evidence Of the Anti-infective Prophylactic Therapy Of Coatingmentioning

confidence: 99%

Coatings as the useful drug delivery system for the prevention of implant-related infections

Pan

Zhou

2018

J Orthop Surg Res

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“…Titanium (Ti) and its alloys are one of the most important metals used in orthopaedic implants due to favourable properties of high strength, rigidity, fracture toughness and their reliable mechanical performance as replacement for hard tissues123. Now main clinical applications of titanium implants in orthopaedics include artificial joints, spinal fusion instruments, and fracture fixations such as plates, screws and intramedullary rods4567.…”

mentioning

confidence: 99%

Novel Bio-functional Magnesium Coating on Porous Ti6Al4V Orthopaedic Implants: In vitro and In vivo Study

Gao

Wan

et al. 2017

Sci Rep

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Titanium and its alloys with various porous structures are one of the most important metals used in orthopaedic implants due to favourable properties as replacement for hard tissues. However, surface modification is critical to improve the osteointegration of titanium and its alloys. In this study, a bioactive magnesium coating was successfully fabricated on porous Ti6Al4V by means of arc ion plating, which was proved with fine grain size and high film/substrate adhesion. The surface composition and morphology were characterized by X-ray diffraction and SEM equipped with energy dispersive spectroscopy. Furthermore, the in vitro study of cytotoxicity and proliferation of MC3T3-E1 cells showed that magnesium coated porous Ti6Al4V had suitable degradation and biocompatibility. Moreover, the in vivo studies including fluorescent labelling, micro-computed tomography analysis scan and Van-Gieson staining of histological sections indicated that magnesium coated porous Ti6Al4V could significantly promote bone regeneration in rabbit femoral condylar defects after implantation for 4 and 8 weeks, and has better osteogenesis and osteointegration than the bare porous Ti6Al4V. Therefore, it is expected that this bioactive magnesium coating on porous Ti6Al4V scaffolds with improved osteointegration and osteogenesis functions can be used for orthopedic applications.

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“…Since 2005 this coating is used on intramedullary tibia nails loaded with gentamicin for infection prophylaxis 41 . But several other coating techniques are investigated in preclinical studies 42–45 . To combine the antimicrobial treatment with defect regeneration or in prosthetic surgery, other applications are also possible such as perioperative mixing of the AMP with bone grafting material, such as autologous or allogeneic bone, polymers, tricalciumphosohate, bioglass or cement 46, 47 .…”

Section: Discussionmentioning

confidence: 99%

A short artificial antimicrobial peptide shows potential to prevent or treat bone infections

Bormann

Koliszak

Kasper

et al. 2017

Sci Rep

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Infection of bone is a severe complication due to the variety of bacteria causing it, their resistance against classical antibiotics, the formation of a biofilm and the difficulty to eradicate it. Antimicrobial peptides (AMPs) are naturally occurring peptides and promising candidates for treatment of joint infections. This study aimed to analyze the effect of short artificial peptides derived from an optimized library regarding (1) antimicrobial effect on different bacterial species, (2) efficacy on biofilms, and (3) effect on osteoblast-like cells. Culturing the AMP-modifications with Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus (including clinical isolates of MRSA and MSSA) and Staphylococcus epidermidis identified one candidate that was most effective against all bacteria. This AMP was also able to reduce biofilm as demonstrated by FISH and microcalorimetry. Osteoblast viability and differentiation were not negatively affected by the AMP. A cation concentration comparable to that physiologically occurring in blood had almost no negative effect on AMP activity and even with 10% serum bacterial growth was inhibited. Bacteria internalized into osteoblasts were reduced by the AMP. Taken together the results demonstrate a high antimicrobial activity of the AMP even against bacteria incorporated in a biofilm or internalized into cells without harming human osteoblasts.Infections of bone are a severe complication and can result in a long-term treatment. Infected/contaminated material (tissue and implant) must be removed and the infection cleared. Staphylococcus aureus is the predominant bacteria identified in fracture-fixation device associated infections (30%) followed by coagulase-negative staphylococci (22%), but also Gram-negative bacteria, enterococci, streptococci and other species may be isolated 1 . The interaction of S. aureus with bone or especially osteoblasts is well investigated 2 . For example, S. aureus expresses several molecules (collagen adhesin, bone sialoprotein binding protein, MHC II analog protein) that support the binding with proteins of the extracellular matrix of bone, but also induces the secretion of inflammatory factors and chemokines, and can induce apoptosis. A mechanism that allows S. aureus to persist protected from the immune system and most antibiotics is the internalization into host cells, especially osteoblast 3 . Within the last decades the number of resistant bacteria increased, resulting in more challenges for the treatment. Facing the impact of resistant bacteria on the human health and the health care systems, antimicrobial resistance (AMR) is identified as one of the major health problems 4 resulting in the endorsement of a global action plan by the World Health Organization to tackle antimicrobial resistance. Besides the need to increase the awareness to this problem and the improvement of the antibiotic use, new antimicrobial active drugs must be developed. An interesting group of antimicrobial active drugs are antimicrobial pep...

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Gentamicin coating of plasma chemical oxidized titanium alloy prevents implant-related osteomyelitis in rats

Cited by 80 publications

References 28 publications

Coatings as the useful drug delivery system for the prevention of implant-related infections

Coatings as the useful drug delivery system for the prevention of implant-related infections

Novel Bio-functional Magnesium Coating on Porous Ti6Al4V Orthopaedic Implants: In vitro and In vivo Study

A short artificial antimicrobial peptide shows potential to prevent or treat bone infections

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