Gentamicin-resistant<i>Salmonella typhimurium</i>phage type 204c: molecular studies and strain diversity in a putative bovine outbreak

Platt, D. J.; Smith, Ifeanyi Stella

doi:10.1017/s0950268800048834

Cited by 7 publications

(8 citation statements)

References 16 publications

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“…Plasmid REFP with the restriction enzyme SmaI was performed on all isolates that were shown to be positive for integron DNA [9]. To determine whether the integrons were plasmid-or chromosomally located, conjugation to a plasmid-free strain of Escherichia coli K12, J53-2, was performed as previously described [10]. Potential transconjugants were lactose-fermenting colonies on CLED agar and resistant to rifampicin and ampicillin or streptomycin.…”

Section: Conjugation and Restriction Enzyme Fragmentation Pattern (Rementioning

confidence: 99%

Detection and characterisation of integrons in Salmonella enterica serotype Enteritidis

Brown

2000

FEMS Microbiology Letters

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Integrons have been widely described among the Enterobacteriaceae including strains of multi-resistant Salmonella enterica serotype Typhimurium DT104; however, information with respect to the presence of integrons among S. enterica serotype Enteritidis strains is limited. Multi-resistant isolates of Enteritidis were screened for the presence of integrons using a PCR protocol. One integron was detected in all isolates that were resistant to sulfonamide and streptomycin. Characterisation of these isolates indicated an integron which ranged in size between 1000 and 2000 bp and which harboured a gene cassette encoding the ant(3Q)-Ia gene specifying streptomycin and spectinomycin resistance. Further studies revealed the integrons to be located on large conjugative plasmids. This appears to be the first report of plasmidborne integrons in Enteritidis. ß

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Section: Conjugation and Restriction Enzyme Fragmentation Pattern (Rementioning

confidence: 99%

Detection and characterisation of integrons in Salmonella enterica serotype Enteritidis

Brown

2000

FEMS Microbiology Letters

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“…The IncX R-plasmid, pOG670, and a cointegrate (pOG669) formed between this plasmid and the virulence plasmid of Typhimurium have previously been described by Platt and Smith [17]. During a study of the virulence determining properties of these plasmids [18], it was noted that the plasmids, after conjugal transfer into Enteritidis recipients, altered the phage type.…”

Section: Introductionmentioning

confidence: 99%

Phage type conversion in Salmonella enterica serotype Enteritidis caused by the introduction of a resistance plasmid of incompatibility group X (IncX)

Brown

Baggesen²,

Platt

et al. 1999

Epidemiol. Infect.

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The plasmid pOG670, a 54 kb, conjugative plasmid that specifies resistance to ampicillin and kanamycin and belonging to the incompatibility group X (IncX), was transferred into 10 isolates of Salmonella enterica serotype Enteritidis belonging to 10 different phage types (PT1, 2, 3, 4, 8, 9, 9b, 10, 11 and 13). Acquisition of the plasmid by these strains did not result in the loss of any resident plasmids but resulted in phage type conversion in 8 of the 10 strains (PT1, 2, 4, 8, 9, 9b, 10 and 11). The observed changes in phage type were found to result from the loss of sensitivity to 3 of the 10 typing phages used (phages 3, 5 and 7). Where the conversion resulted in a change to a defined phage type, both the new and original PTs belonged to the same, previously described, evolutionary lines. Enteritidis PTs 1, 4 and 8, commonly associated with poultry world-wide, were converted to PTs 21, 6 and 13a respectively. The results indicate a different route for phage type conversion Enteritidis from others reported in the literature and, although IncX plasmids are not normally present in PT8 or PT13a, may suggest a possible mechanism/link connecting these phage types.

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“…Although this report has focused on molecular variation in the SSP of Enteritidis this plasmid is nevertheless highly conserved on the basis of REFP analysis like the SSP of Typhimurium [5] and in marked contrast to resistance plasmids [31,32]. Whereas the conservation of a virulence region might be expected and this has been shown to be about 8 kb, the conservation of a further 46 kb (Enteritidis) and 82 kb (Typhimurium) is more surprising.…”

Section: Discussionmentioning

confidence: 99%

The distribution of serotype-specific plasmids among different subgroups of strains ofSalmonella entericaserotype Enteritidis: characterization of molecular variants by restriction enzyme fragmentation patterns

1995

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SUMMARYFour hundred and thirty-four isolates of Salmonella enterica serotype Enteritidis were studied. They were grouped into five subsets defined by either the collection criteria or the parameter which formed the basis for subsequent analysis. Seventyseven per cent harboured the serotype-specific plasmid (SSP). In 55% of the isolates this was the sole plasmid. Molecular variation in the SSP was detected in 17 (5%) of the isolates on the basis of restriction enzyme fragmentation pattern (REFP) analysis using Pst I and Sma I. The SSP variants were further characterized using additional restriction enzymes chosen to optimize the information content and analysed using a coefficient of similarity.A variant SSP designated pOG690 showed greater resemblance to the SSP of Salmonella enterica serotype Typhimurium than Enteritidis; 89 % and 68 % respectively for Pst I and 79 % and 55 % respectively for Sma I. In respect of the Pst I data pOG690 shared at least 55 kb of DNA with the Typhimurium SSP and 37 kb with the SSP of Enteritidis. This variant was associated with poultry (duck, goose, chicken) and all isolates belonged to phage type 9b. Other variants were associated with phage types 4, 6, 6a, 9a, 11, 15 and 24. The epidemiological implications of these results are discussed.

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Gentamicin-resistantSalmonella typhimuriumphage type 204c: molecular studies and strain diversity in a putative bovine outbreak

Cited by 7 publications

References 16 publications

Detection and characterisation of integrons in Salmonella enterica serotype Enteritidis

Detection and characterisation of integrons in Salmonella enterica serotype Enteritidis

Phage type conversion in Salmonella enterica serotype Enteritidis caused by the introduction of a resistance plasmid of incompatibility group X (IncX)

The distribution of serotype-specific plasmids among different subgroups of strains ofSalmonella entericaserotype Enteritidis: characterization of molecular variants by restriction enzyme fragmentation patterns

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