Gentiopicroside prevents alcoholic liver damage by improving mitochondrial dysfunction in the rat model

Yang, Xiaomei; Wang, Shuang; Song, Shuang; Yang, Xiudong

doi:10.1002/ptr.6981

Cited by 29 publications

(17 citation statements)

References 64 publications

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“…Mitochondria were isolated from the 15-min ischemic myocardium using a mitochondrial isolation kit (KeyGEN, KGA827, Jiangsu, China) as previously reported [ 36 ]. Procedure modification was made according to the manufacture’s instruction.…”

Section: Methodsmentioning

confidence: 99%

Identification of the metabolic remodeling profile in the early-stage of myocardial ischemia and the contributory role of mitochondrion

Liu

Wang

et al. 2022

Bioengineered

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Cardiac remodeling is the primary pathological feature of chronic heart failure. Prompt inhibition of remodeling in acute coronary syndrome has been a standard procedure, but the morbidity and mortality are still high. Exploring the characteristics of ischemia in much earlier stages and identifying its biomarkers are essential for introducing novel mechanisms and therapeutic strategies. Metabolic and structural remodeling of mitochondrion is identified to play key roles in ischemic heart disease. The mitochondrial metabolic features in early ischemia have not previously been described. In the present study, we established a mouse heart in early ischemia and explored the mitochondrial metabolic profile using metabolomics analysis. We also discussed the role of mitochondrion in the global cardiac metabolism. Transmission electron microscopy revealed that mitochondrial structural injury was invoked at 8 minutes post-coronary occlusion. In total, 75 metabolites in myocardium and 26 in mitochondria were screened out. About 23% of the differentiated metabolites in mitochondria overlapped with the differentiated metabolites in myocardium; Total 81% of the perturbed metabolic pathway in mitochondria overlapped with the perturbed pathway in myocardium, and these pathways accounted for 50% of the perturbed pathway in myocardium. Purine metabolism was striking and mechanically important. In conclusion, in the early ischemia, myocardium exacerbated metabolic remodeling. Mitochondrion was a contributor to the myocardial metabolic disorder. Purine metabolism may be a potential biomarker for early ischemia diagnosis. Our study introduced a perspective for prompt identification of ischemia.

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Section: Methodsmentioning

confidence: 99%

Identification of the metabolic remodeling profile in the early-stage of myocardial ischemia and the contributory role of mitochondrion

Liu

Wang

et al. 2022

Bioengineered

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“…Ni 2+ treatment was applied as described for the oxidative stress analysis experiment [ 8 ]. After the cells had been cultured for a given time (12, 24, 48 or 72 h), JC-1 and Hoechst staining was performed in turn, and a high-content cell analysis system was used to determine the fluorescence intensities of the JC-1 monomer (green fluorescence) and polymer (red fluorescence).…”

Section: Methodsmentioning

confidence: 99%

Screening and validation of nickel ion cytotoxicity biomarkers based on transcriptomic and proteomic technology

Zhang

Huang

Lü

2022

Regenerative Biomaterials

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The aim of this study was to screen cytotoxicity biomarkers of nickel ions (Ni2+) using transcriptomic and proteomic approaches combined with molecular biology validation. First, the MTT method was used to evaluate cytotoxicity in L929 cells treated with Ni2+ at different concentrations. Ni2+ at both 100 μM and 200 μM affected cell proliferation. Then, transcriptomic and proteomic technology was used to study the effects of Ni2+ on the expression of genes/proteins in cells. It was found that 1490, 789, 652 and 729 genes (12, 24, 48 and 72 h, respectively) and 177, 2191 and 2095 proteins (12, 24 and 48 h, respectively) were differentially expressed after treatment with 100 μM Ni2+. In total, 1403, 963, 916 and 1230 genes (12, 24, 48 and 72 h, respectively) and 83, 1681 and 2398 proteins (12, 24 and 48 h, respectively) were differentially expressed after treatment with 200 μM Ni2+. Then, 4 target gene/protein biomarkers were filtered by combined screening using gene/proteomic experimental data and biological pathway analyses. Further expression level validation of all these target biomarkers and functional validation of selected gene/protein biomarkers were carried out, and a final gene/protein biomarker (UQCRB) was identified.

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“…What’s more, Gentiopicroside not only protects alcoholic liver disease by improving lipid metabolism imbalance and mitochondrial dysfunction caused by alcohol ( Yang, H.-X. et al, 2020 ; Zhang et al, 2021 ), but also treats alcoholic liver cancer by regulating the activation of P2x7R-NLRP3 inflammasome ( Li, Xia et al, 2018 ). It is worth mentioning that astragaloside IV can inhibit hepatoma cells by inhibiting multidrug resistance-associated protein 2, and long noncoding RNA ATB ( Li, Y. et al, 2018 ; Qu et al, 2020 ).…”

Section: Natural Agents From CM For Liver Disease Treatmentmentioning

confidence: 99%

The Potential Application of Chinese Medicine in Liver Diseases: A New Opportunity

Wang

et al. 2021

Front. Pharmacol.

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Liver diseases have been a common challenge for people all over the world, which threatens the quality of life and safety of hundreds of millions of patients. China is a major country with liver diseases. Metabolic associated fatty liver disease, hepatitis B virus and alcoholic liver disease are the three most common liver diseases in our country, and the number of patients with liver cancer is increasing. Therefore, finding effective drugs to treat liver disease has become an urgent task. Chinese medicine (CM) has the advantages of low cost, high safety, and various biological activities, which is an important factor for the prevention and treatment of liver diseases. This review systematically summarizes the potential of CM in the treatment of liver diseases, showing that CM can alleviate liver diseases by regulating lipid metabolism, bile acid metabolism, immune function, and gut microbiota, as well as exerting anti-liver injury, anti-oxidation, and anti-hepatitis virus effects. Among them, Keap1/Nrf2, TGF-β/SMADS, p38 MAPK, NF-κB/IκBα, NF-κB-NLRP3, PI3K/Akt, TLR4-MyD88-NF-κB and IL-6/STAT3 signaling pathways are mainly involved. In conclusion, CM is very likely to be a potential candidate for liver disease treatment based on modern phytochemistry, pharmacology, and genomeproteomics, which needs more clinical trials to further clarify its importance in the treatment of liver diseases.

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Gentiopicroside prevents alcoholic liver damage by improving mitochondrial dysfunction in the rat model

Cited by 29 publications

References 64 publications

Identification of the metabolic remodeling profile in the early-stage of myocardial ischemia and the contributory role of mitochondrion

Identification of the metabolic remodeling profile in the early-stage of myocardial ischemia and the contributory role of mitochondrion

Screening and validation of nickel ion cytotoxicity biomarkers based on transcriptomic and proteomic technology

The Potential Application of Chinese Medicine in Liver Diseases: A New Opportunity

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