Bedding-parallel fibrous calcite veins crop out at two Permian carbonate localities in the Phetchabun area, central Thailand, within the Nam Duk and Khao Khwang Formations. Samples are studied to determine their petrographic, geochemical and isotopic character, depositional and diagenetic associations and controls on the formation of fibrous calcite across the region. Biomarker and non-biomarker parameters are used to interpret organic matter sources in the vein-hosting units, the depositional environment and levels of source rock maturation in order to evaluate source rock potential in the two Formations. Carbon and oxygen isotope values of the veins and the host are determined to discuss the source of carbonates and diagenetic conditions. The petroleum assessment from the Khao Khwang and Nam Duk Formations suggests that both Formations are a petroleum potential source rock with type II/III kerogen deposited in an estuarine environment or a shallow marine environment and a slope-to-basin marine environment or an open marine environment, respectively. The bedding-parallel fibrous calcite veins from the Khao Khwang and Nam Duk Formations are divided into two types: 1) beef and, 2) cone-in-cone veins. The carbon and oxygen isotope compositions from the fibrous calcite veins suggest that the calcite veins could be precipitated from a carbon source generated in the microbial methanogenic zone. The results in this study provide a better understanding of the interrelationship between the bedding-parallel fibrous calcite veins and petroleum source rock potential.