The ophiolites from the Yarlung Zangbo River (Tibet), Southwestern China, were analysed for the contents of helium and neon and their isotopic compositions by stepwise heating. The serpentinites from Bainang showed a high 3 He/ 4 He value of 32.66R a (R a is referred to the 3 He/ 4 He ratio in the present air) in 700 fraction.℃ At lower temperature, all of the dolerites displayed as very high 3 He/ 4 He ratios as ones investigated for hotspots. It was clear that the high 3 He/ 4 He ratio was one of immanent characterics in the magma source formed the dolerites, suggesting that there was a large amount of deep mantle fluids in these rocks. In the three-isotope diagram of neon, the data points from the ophiolites of the Yarlung Zangbo River were arranged along the Loihi Line. This is in agreement with the characteristics of helium isotopes, revealing that the high-3 He plume from deep mantle had played an important role in the formation of the Neo-Tethyan Ocean. The helium isotopic compositions in the basalts were far higher than atomospheric value but lower than the average value of MORB, although there were various degrees of alteration. The possible reasons were that basaltic magmas had been contaminated by crust-derived fluids.Yarlung Zangbo River (Tibet), ophiolite, helium, neon, isotope, plume It is well known that noble gases are chemically inert, and strongly evoloved with special unclear processes in their generation. Thus the variations in isotopic compositions of noble gases are probably remarkable during a geological process, especially when the variations in Sr, Nd and Pb isotopic compositions are less clearly distiguishable. The distribution characteristics of noble gases isotopic compositions have been widely used to trace the conditions for old ocean-formation and the interactions between crust and mantle, to investigate the interior earth structure, to understand the kinetics during the formation and evolvation of old oceans, to judge elementary enrichment and ore-formation mechanism, and to clarify the inhomogeneity of earth mantle.The Yarlung Zangbo Suture Zone (YZSZ), generally accepted as the collision site between the India and Asia plates, is an extremely complicated tectonic zone and attracts more attention from international geoscientific community because of its unique geological history and crust-mantle structure. The Yarlung Zangbo River ophiolite, outcropped along the Yarlung Zangbo River, is a special marker on the suture zone and a window on measuring various geological issues related with the suture zone. Based on field investigations, many scholars discussed the petrology, magmatism and metamorphism of this suture zone as reviewed by Zhang and Zhou [1] . However, there are so far very limited data on the noble gases isotopes of the ophiolites from this area [2] . How was the Neo-Tethys Ocean represented by the Yarlung Zangbo River ophiolite formed? What is the