An integrated approach considering facies, isotopic, and palynological analyses of lake sediments from the Serra Norte de Carajás, southeastern Amazonia, is presented in this work to refine paleoclimate and paleohydrological changes based on upland lake sediments during the late Quaternary. The sediments have a fining-upward deposition cycle typical of upland swamps/lakes. The origin of organic matter is autochthonous mainly related to C 3 terrestrial plants, macrophytes and algae. The pollen records of Hedyosmum during the Early Pleistocene suggest lower temperatures than those observed along Holocene. In the transitional period between the Pleistocene and the Holocene, rainfall decreased, causing the retraction of the flooded area, favoring the development of marshy conditions. The Late and Middle Holocene were marked by higher temperatures and lower humidity. Afterward, the increased pollen concentration from canga and forest vegetation, macrophytes, palms, and algae suggested increased humidity in the Early Holocene. The relative contribution of forest pollen along the records indicated that drier conditions were not strong enough for an extensive expansion of canga over forested areas.