Geodemographic analysis of Borrelia burgdorferi sensu lato using the 5S–23S rDNA spacer region

Coipan, Elena Claudia; Fonville, Manoj; Tijsse-Klasen, Ellen; Giessen, J.W.B. van der; Takken, Willem; Sprong, Hein; Takumi, Katsuhisa

doi:10.1016/j.meegid.2013.04.009

Cited by 55 publications

(59 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The genetic characterization of the isolated strains showed that the population analysis of 5S-23S rRNA sequences enables the detection of genetic variants that can further resolve subpopulations within a genospecies, defined by e.g. geographical location, as proposed by other studies [26,27]. In accordance, B. turdi 5S-23S rRNA sequences show high variability with 12 5S-23S rRNA polymorphisms detected from only four study areas in central West Portugal [14, 12, this study].…”

Section: Discussionsupporting

confidence: 68%

Characterization Through Multilocus Sequence Analysis of Borrelia turdi Isolates from Portugal

et al. 2015

View full text Add to dashboard Cite

Borrelia turdi is a spirochete from the Borrelia burgdorferi complex, first reported in Japan, that has been increasingly detected in Europe. This genospecies is mostly associated with avian hosts and their ornithophilic ticks such as Ixodes frontalis. In this study, we isolated B. turdi from five I. frontalis feeding on Turdus merula, Turdus philomelos, Parus major and Troglodytes troglodytes, and one Ixodes ricinus feeding on a T. merula in Portugal. These isolates were genetically characterised according to their 5S-23S rRNA intergenic spacer, 16S rRNA and through typing of seven housekeeping genes (multilocus sequence typing). Multilocus sequence analyses revealed that the strains isolated in our study, although belonging to B. turdi genospecies, are not identical to the B. turdi reference strain Ya501. Instead, our strains are separated into a clear defined group, suggesting that the European samples diverged genetically from the strain originally detected in Japan. Population analysis of 5S-23S rRNA sequences can further resolve subpopulations within B. turdi, but more samples from a large geographical scale and host range would be needed to assess potential phylogeographical patterns within this genospecies.

show abstract

Section: Discussionsupporting

confidence: 68%

Characterization Through Multilocus Sequence Analysis of Borrelia turdi Isolates from Portugal

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The PCR was performed according to the protocol described in Coipan et al (2013a). Alignment of the sequences was made using MAFFT (Katoh and Toh, 2008) and the sequences were trimmed to nucleotides between position 438,838 and 439,196 (359 nucleotides) of whole genome sequence of B. afzelii strain PKo (GenBank entry CP002933).…”

Section: S-23s Typingmentioning

confidence: 99%

“…Previous studies (Coipan et al, 2013a) have shown that the 5S-23S rDNA intergenic spacer (IGS) can also discriminate among the genospecies of B. burgdorferi s.l. and that it can also detect genetic differentiation among the bacteria of various geographic provenience.…”

Section: Introductionmentioning

confidence: 99%

Imbalanced presence of Borrelia burgdorferi s.l. multilocus sequence types in clinical manifestations of Lyme borreliosis

Coipan

Jahfari

Fonville

et al. 2016

Infection, Genetics and Evolution

Self Cite

View full text Add to dashboard Cite

In this study we used typing based on the eight multilocus sequence typing scheme housekeeping genes (MLST) and 5S-23S rDNA intergenic spacer (IGS) to explore the population structure of Borrelia burgdorferi sensu lato isolates from patients with Lyme borreliosis (LB) and to test the association between the B. burgdorferi s.l. sequence types (ST) and the clinical manifestations they cause in humans. Isolates of B. burgdorferi from 183 LB cases across Europe, with distinct clinical manifestations, and 257 Ixodes ricinus lysates from The Netherlands, were analyzed for this study alone. For completeness, we incorporated in our analysis also 335 European B. burgdorferi s.l. MLST profiles retrieved from literature. Borrelia afzelii and Borrelia bavariensis were associated with human cases of LB while Borrelia garinii, Borrelia lusitaniae and Borrelia valaisiana were associated with questing I. ricinus ticks. B. afzelii was associated with acrodermatitis chronica atrophicans, while B. garinii and B. bavariensis were associated with neuroborreliosis. The samples in our study belonged to 251 different STs, of which 94 are newly described, adding to the overall picture of the genetic diversity of Borrelia genospecies. The fraction of STs that were isolated from human samples was significantly higher for the genospecies that are known to be maintained in enzootic cycles by mammals (B. afzelii, B. bavariensis, and Borrelia spielmanii) than for genospecies that are maintained by birds (B. garinii and B. valaisiana) or lizards (B. lusitaniae). We found six multilocus sequence types that were significantly associated to clinical manifestations in humans and five IGS haplotypes that were associated with the human LB cases. While IGS could perform just as well as the housekeeping genes in the MLST scheme for predicting the infectivity of B. burgdorferi s.l., the advantage of MLST is that it can also capture the differential invasiveness of the various STs.

show abstract

“…N. mikurensis DNA in a duplex qPCR 233 . For typing to the genospecies level of B. burgdorferi s.l., the positive samples of the qPCR were further submitted to PCR targeting the variable 5S-23S intergenic spacer region (IGS), according to the protocol described in Coipan et al 234 . We sequenced PCR products using an ABI PRISM BigDye Terminator Cycle sequencing Ready Reaction kit (Perkin Elmer, Applied Biosystems), confirmed the sequences by sequencing both strands 235 , and identified the Borrelia genospecies based on the DNA sequence of IGS.…”

Section: Density Of Infected Nymphsmentioning

confidence: 99%

“…was below 8%, an extra set was tested to increase precision. We used the methods described in Coipan et al 234 , Jahfari et al 51 , and Chapter 6 to determine the nymphal infection prevalence (NIP) for Anaplasma phagocytophilum, Borrelia afzelii, B. burgdorferi s.s., B. garinii, B. valaisiana, B. miyamotoi, and Candidatus Neoehrlichia mikurensis. NIP values were multiplied with the density of nymphs to calculate the density of infected nymphs (DIN; 100 m -2 ).…”

Section: Pathogen Prevalence In Ticksmentioning

confidence: 99%

The wild life of tick-borne pathogens

Hofmeester

View full text Add to dashboard Cite

Geodemographic analysis of Borrelia burgdorferi sensu lato using the 5S–23S rDNA spacer region

Cited by 55 publications

References 44 publications

Characterization Through Multilocus Sequence Analysis of Borrelia turdi Isolates from Portugal

Characterization Through Multilocus Sequence Analysis of Borrelia turdi Isolates from Portugal

Imbalanced presence of Borrelia burgdorferi s.l. multilocus sequence types in clinical manifestations of Lyme borreliosis

The wild life of tick-borne pathogens

Contact Info

Product

Resources

About