Geodermatophilus tzadiensis sp. nov., a UV radiation-resistant bacterium isolated from sand of the Saharan desert

Abstract

Three novel Gram-positive, aerobic, actinobacterial strains, CF5/2(T), CF5/1 and CF7/1, were isolated in 2007 during environmental screening of arid desert soil in the Sahara desert, Chad. Results from riboprinting, MALDI-TOF protein spectra and 16S rRNA sequence analysis confirmed that all three strains belonged to the same species. Phylogenetic analysis of 16S rRNA sequences with the strains' closest relatives indicated that they represented a distinct species. The three novel strains also shared a number of physiological and biochemical characteristics distinct from previously named Geodermatophilus species. The novel strains' peptidoglycan contained meso-diaminopimelic acid; their main phospholipids were phosphatidylcholine, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and a small amount of phosphatidylglycerol; MK-9(H4) was the dominant menaquinone. The major cellular fatty acids were the branched-chain saturated acids iso-C16:0 and iso-C15:0. Galactose was detected as diagnostic sugar. Based on these chemotaxonomic results, 16S rRNA gene sequence analysis and DNA-DNA hybridization between strain CF5/2(T) and the type strains of Geodermatophilus saharensis, Geodermatophilus arenarius, Geodermatophilus nigrescens, Geodermatophilus telluris and Geodermatophilus siccatus, the isolates CF5/2(T), CF5/1 and CF7/1 are proposed to represent a novel species, Geodermatophilus tzadiensis, with type strain CF5/2(T)=DSM 45416=MTCC 11411 and two reference strains, CF5/1 (DSM 45415) and CF7/1 (DSM 45420).

Geodermatophilus poikilotrophisp. nov.: A Multitolerant Actinomycete Isolated from Dolomitic Marble

“…Young colonies were light-red in colour and turned greenish-black at maturity. Similar colours conversions were already observed by Nie et al [17] and Montero-Calasanz et al [18, 19, 21, 22, 25] for type strains of other representatives of the genus, such as G. nigrescens , G. arenarius, G. siccatus, G. saharensis, G. tzadiensis, and G. normandii , when cultivated under the same growth conditions (Table 1). Colonies were convex, nearly circular and opaque with a moist surface and an entire margin.…”

“…Fourteen named species have been classified in the genus Geodermatophilus (ordered by the dates of effective publication of the names): G. obscurus [3], G. ruber [9], G. nigrescens [17], G. arenarius [18], G. siccatus [19, 20], G. saharensis [20, 21], G. tzadiensis [22, 23], G. telluris [24], G. soli and G. terrae [10], G. africanus [5, 23], G. normandii [25], G. taihuensis [26], and G. amargosae [27, 28]. Until now, only the genome of the type strain of the type species, G. obscurus G-20 T , has been sequenced [29].…”

Geodermatophilus brasiliensis sp. nov., isolated from Brazilian soil

“…everesti' (Ishiguro & Fletcher, 1975;Normand & Benson, 2012), remained the only representatives of the genus in the literature. This has changed dramatically in the last 3 years, when technical limitations were overcome and not only the first genome of a strain from the genus was sequenced, that of G. obscurus G-20, by Ivanova et al (2010), but 12 novel species have been assigned to the genus (in date order of effective publication): Geodermatophilus ruber (Zhang et al, 2011), G. nigrescens (Nie et al, 2012), G. arenarius (Montero-Calasanz et al, 2012), G. siccatus (Montero-Calasanz et al, 2013a), G. saharensis (Montero-Calasanz et al, 2013b), G. tzadiensis (Montero-Calasanz et al, 2013c), G. telluris (MonteroCalasanz et al, 2013d), G. soli and G. terrae (Jin et al, 2013) (Montero-Calasanz et al, 2013f) and G. taihuensis (Qu et al, 2013). Recently, Geodermatophilus amargosae (MonteroCalasanz et al, 2014a, b) was described in detail to elevate 'G.…”

Spatial distribution of microbial communities associated with dune landform in the Gurbantunggut Desert, China

“…(2.5-8.0%) than that in LC-BSCs (0.5-0.9%), implying that they had important roles during the formation of cyanobacterial crusts. Genus Geodermatophilus was first described by Leudemann (1968), and recently several strains have been isolated from deserts with various resistances such as xerotolerance, UV radiation-resistance and halotolerance (Montero-Calasanz et al 2012, 2013a. Cyanobacterial C-fixation might contribute to the frequent occurrence of heterotrophic Geodermatophilus bacteria in HB-BSCs.…”