The western succession of the Mount Isa basin in northwest Queensland hosts four supergiant Zn-Pb ± Cu deposits and numerous smaller Cu and Zn-Pb deposits. Mineralization is primarily hosted in carbonaceous and calcareous shales and siltstones belonging to the 1670 to 1575 Ma Isa superbasin, but little is known about the source of metals that formed these deposits. The underlying clastic and volcanic successions belonging to the 1800 to 1750 Ma Leichhardt superbasin and the 1735 to 1690 Ma Calvert superbasin are potential metal source rocks and host a variety of diagenetic minerals that preserve geochemical information about the evolution of brines in the basin.Quartz overgrowths and pressure solution features formed during shallow burial in all clastic lithologic units but are particularly common in the well-sorted, marine-dominated units that became aquitards due to the porosity-occluding diagenetic cement. Microthermometry on fluid inclusions in the quartz overgrowths indicates formation between 100º and 174ºC from a low-salinity, 2.7 to 9.1 wt percent NaCl equiv fluid (fluid 1). These data together with sequence stratigraphic mapping, basin reconstruction, and stable isotope values from the quartz overgrowths show that the diagenetic aquitards formed at <5-km depth. Illite and chlorite formed locally in some of the diagenetic aquitards and preserve δ 18 Ofluid and δDfluid values of 4.5 ± 4.2 and -34 ± 14 per mil, respectively, indicating evolution from a seawater-dominated source. Silicate dissolution and the widespread formation of diagenetic illite and chlorite occurred late, during deep burial diagenesis and primarily in the proximal fluvial lithologic units. These units are recognized as diagenetic aquifers and they occur adjacent to and within the Eastern Creek and Fiery Creek Volcanics where metals could have been sourced. Basin reconstruction shows that the diagenetic aquifers formed at depths between 5 and 10 km. Illite and chlorite extracted from the diagenetic aquifers have distinct δ 18 Ofluid and δDfluid values of 4.5 ± 2.8 and -63 ± 11 per mil, respectively, indicating evolution from a meteoric fluid with a variable marine contribution. These isotopic values cannot be differentiated from published isotopic values of fluid inclusion water in quartz-dolomite-chalcopyrite veins at Mount Isa or sphalerite and illite from the Century Zn deposit and the Zn lodes from the Burketown mineral field. This suggests that the diagenetic aquifers were likely source rocks for metals in the deposits in the Mount Isa basin. In contrast to the phyllosilicates in the diagenetic aquifers, regional dolomitic grainstones and dolomudstones in the Lawn Hill platform precipitated from fluids with δ 18 Ofluid between -2.6 and 1.1 per mil and δ 13 Cfluid between -8.6 and -3.9 per mil. This suggests that these units did not contribute to the ore-forming brines.Quartz veins formed during the later diagenetic history in the Mount Isa basin from a low-salinity brine, between 2.7 and 10.4 wt percent NaCl equiv (fluid 2). Oxygen ...