Geographical and Environmental Variation in Chemical Constituents and Antioxidant Properties inRoscoea proceraWall

Abstract

Roscoea procera is an important Himalayan medicinal plant known for its food and medicinal potential due to antioxidant activity. Among its wild populations, total phenolic and flavonoid content in the rhizomes varied significantly (P < 0.05). RP-HPLC analysis of phenolic compounds revealed high variation (P < 0.05) among populations in gallic acid, catechin and p-coumaric acid content. Antioxidant activities determined by different in vitro assays, (ABTS, DPH and FRAP assays) showed the antioxidant potential of the species. Data on different forest types revealed that populations under open grassy land possess higher level of antioxidants. High level of phenolic antioxidant in certain populations identified by neighbor joining clustering and equal class distribution analysis indicated some potential populations as a source for natural antioxidant and variability among populations can be further used for selection of elite for future breeding programmes. PRACTICAL APPLICATIONSRoscoea procera Wall is a medicinally important Himalayan herbs found in an altitudinal range of 1800-3000 m asl in different habitats conditions. The rhizomes of the species are directly collected from wild for many traditional medicines and edible food products due to its antioxidant properties. In the present study, antioxidant properties (ABTS and DPPH radical scavenging activity and ferricreducing antioxidant potential), phenolic content and composition of dried rhizomes were analysed, which may provide possible role of the species against health benefits and suggest future applications in functional foods development. In addition, the variation in antioxidant properties, phenolic content and other phytochemical composition in different populations and environmental conditions may provide baseline data for future breeding programmes for variety improvement and suitable conditions for cultivation.

Coronarin K and L: Two Novel Labdane Diterpenes From Roscoea purpurea: An Ayurvedic Crude Drug

“…In view of its significance in the Ayurvedic medicinal system, substantial pharmacological and phytochemical works have not been carried out. Previous phytochemical studies on R. purpurea have described the isolation of two principal groups of compounds: steroids and phenolic derivatives (Misra et al, 2015 ; Srivastava et al, 2015 ; Rawat et al, 2016 ). To date, only a few compounds have been identified and quantified through high-performance liquid chromatography (HPLC) analysis from tubers of this plant by Singh and co-workers, and they are presumed to be associated with its potent antioxidant activity (Gopal et al, 2014 ; Rawat et al, 2014 ).…”

“…To date, only a few compounds have been identified and quantified through high-performance liquid chromatography (HPLC) analysis from tubers of this plant by Singh and co-workers, and they are presumed to be associated with its potent antioxidant activity (Gopal et al, 2014 ; Rawat et al, 2014 ). Alcoholic extracts of the plant have shown in vitro anti-cancer (Srivastava et al, 2015 ), antioxidant (Rawat et al, 2016 ), and immunomodulatory activities (Sahu et al, 2010 ). In our preliminary pharmacological study, cytotoxic activity was found for the methanol extract of R. purpurea against lung cancer cell line at IC 50 25.71 (μg/ml).…”

Seasonal variations in harvest index and bacoside A contents amongst accessions of Bacopa monnieri (L.) Wettst. collected from wild populations

“…Variation in the levels of active principle amongst the populations collected from different locations have been reported earlier in many plant species (Bagdonaite et al 2010;Chaves et al 2013;Filippini et al 2010;Nadeem et al 2000Nadeem et al , 2007Soni et al 2015;Rawat et al 2016) including B. monnieri (Naik et al 2012). Naik et al (2012) have studied variations in bacoside A content in the samples collected from different populations growing in a limited area of the homogenous environment from the state of Karnataka in India.…”

“…These populations were evaluated from the samples collected from different regions thus, the observed results could also be due to environmental and edaphic factors (Rawat et al 2016;Cirak et al 2007). A close perusal of literature revealed no report studying the variations in harvestable biomass and levels of bacoside A during different seasons in various populations of B. monnieri collected from different agro climatic zones after these has been maintained at a common location for 1 year.…”

Polyphenolics, Nutrients and Antioxidant Activity of Gaultheria trichophylla Royle: A High Value Wild Edible Plant of Trans Himalaya

“…Similar kind of trends also reported in Guava 32 and Roscoea procera. 33 Elsewhere, studies also indicated that phenolics are positively correlated with antioxidant activity 34,35 and responsible for the maximum activity. …”