Geographical dependence of sequence variation in the E7 gene of human papillomavirus type 16

Eschle, D.; Dürst, Matthias; Meulen, Jan ter; Luande, J.; Eberhardt, Hans; Pawlita, Michael; Gissmann, Lutz

doi:10.1099/0022-1317-73-7-1829

Cited by 57 publications

(56 citation statements)

References 14 publications

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“…G94A was also reported in southwest China and Mongolia (8,35). Sequence variation at E7 displays geographic dependence (14,36). The E7 N29S replacement may be associated with a higher oncogenic risk (12,23,38).…”

Section: Discussionmentioning

confidence: 91%

Human Papillomavirus Type 16 Variant Analysis of E6, E7, and L1 Genes and Long Control Region in Identification of Cervical Carcinomas in Patients in Northeast China

et al. 2011

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Human papillomavirus type 16 (HPV 16) is the primary etiology of cervical cancer, which is the second most common type of cancer in women worldwide (29). HPV 16 variants, which vary by Յ2% from HPV 16 prototype nucleotide sequences, have been identified as the following six phylogenetic branches: European (E), Asian (As), Asian-American (AA), African-1 (Af-1), African-2 (Af-2), and northern American (NA) variants (18,52). Several researchers had reported correlations between specific HPV 16 variants and persistent viral infection, followed by the development of malignant lesions (3,4,16,37,43,49,50). Non-European variants were found to be associated with an excess risk of cervical cancer (37). These variants had been found to show different geographic distributions, while some sequence variations had oncogenic potentials. In HPV 16 variants, the L83V mutation in E6 in the Swedish and Italian populations and D25E in E6 in the Japanese population were reported to be associated with the

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Section: Discussionmentioning

confidence: 91%

Human Papillomavirus Type 16 Variant Analysis of E6, E7, and L1 Genes and Long Control Region in Identification of Cervical Carcinomas in Patients in Northeast China

et al. 2011

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“…Both mutations have been previously reported in HPV-16 variants, without any specific class association, from cervical carcinoma biopsies of non-European geographical regions : 5 out of 11 samples from Panama, Georgia and Alabama (Icenogle et al, 1991) ; 18 out of 22 samples from Tanzania (Eschle et al, 1992) ; and 8 out of 11 samples from Barbados (Smits et al, 1994). The linkage of both mutations to specific mutation patterns in LCR, L1 and\or E5, and to the E6 pattern in the Ugandan samples, suggests that the position 789 and 795 changes are distinctive of the Ax group.…”

Section: Hpv-16 E7 and L1 Sequence Mutationsmentioning

confidence: 99%

“…PCR oligonucleotide primer sequences are summarized in Table 1. HPV-16 DNA nucleotide positions are numbered according to the published sequence of the reference clone (Seedorf et al, 1985), revised as described by Icenogle et al (1991), Chan et al (1992), Eschle et al (1992) and Ho et al (1993). The reaction mixtures (50 µl) for all sets of primers contained 200 ng of target DNA, 20 pmol of each primer, 50 mM KCl, 2n5 mM MgCl # , 100 mM Tris-HCl pH 8n3, 0n1 % Triton X-100, 50 µM of each dNTP and 1n8 units of thermostable Taq DNA polymerase (Perkin-Elmer Cetus).…”

Section: Methodsmentioning

confidence: 99%

“…Ugandan samples do not show the mutation at nt 647 (A to G) identified by Eschle et al (1992) in 8 out of 18 HPV isolates from Tanzanian cervical carcinoma. These eight samples, on the basis of LCR sequence, have been subsequently classified as Af2 variants (Ho et al, 1993).…”

Section: Hpv-16 E7 and L1 Sequence Mutationsmentioning

confidence: 99%

“…The AA, Af1 and Af2 classes form together a coherent phylogenetic group and are referred to as Ax classes (Myers et al, 1994(Myers et al, , 1996. Eschle et al (1992), analysing the nucleotide sequence of the HPV-16 E7 gene in 32 genital tumours collected from Tanzania and from Germany identified two major clusters : the first included all ten German and four Tanzanian HPV-16 isolates, without any nucleotide substitution compared to the prototype ; and the second the remaining 18 Tanzanian HPV-16 isolates, which are characterized by two silent mutations at nt 789 (T to C) and 795 (T to G). Eight samples of the latter cluster show an additional nonsynonymous mutation at nt 647 (A to G).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Sequence variations and viral genomic state of human papillomavirus type 16 in penile carcinomas from Ugandan patients.

Tornesello

Buonaguro

Meglio

et al. 1997

Journal of General Virology

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Establishment and characterization of 12 uterine cervical-carcinoma cell lines: Common sequence variation in theE7 gene of HPV-16-positive cell lines

Kim

Park

et al. 1997

Int. J. Cancer

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Carcinoma of the uterine cervix is one of the most common neoplasms in women, and about 90% of all cervical carcinomas are epidemiologically associated with infection by high-risk human papillomaviruses (HPV) such as types 16, 18, 31 and 33 (Zur Hausen, 1991).Because most available cervical-cancer cell lines were initiated more than 20 years ago (Spence et al., 1988), they have been extensively passaged in culture for hundreds of times in uncontrolled growth conditions, and this might have resulted in changes in the original characteristics of the primary tumor (Spence et al., 1988;Braun et al., 1993). Moreover, because establishment is difficult, the numbers of recently established cell lines with a low passage number are limited (Spence et al., 1988;Braun et al., 1993). The establishment from histopathologically varied primary tumors of cervical-cancer cell lines with a low passage number and an understanding of their characteristics are therefore required.In cervical cancers, a high-risk viral DNA genome is often found to be integrated into the host chromosome by common disruption of the viral E1/E2 region (Schwarz et al., 1985;Chen et al., 1994). In in vitro experiments, E2 repress transcription from the promoters of the E6 and E7 genes. In contrast, E6/E7 genes are actively transcribed in E2-deleted cervical cancers and cell lines (Schwarz et al., 1985).E6 and E7 proteins have been shown to form complexes with the normal cellular protein, p53, or the retinoblastoma-susceptibility gene product, pRb, both of which are well-known tumor suppressors, and those complexes lead to inactivation of the suppression function . Oncogenic HPV-type E6 protein specifically binds to wild-type p53 protein and subsequently enhances the degradation of p53 protein in an ATP/ubiquitin-dependent manner (Scheffner et al., 1990). In addition, pRb is inactivated by binding with E7 protein or through mutation. E7 protein of high-risk HPVs complex with pRb to inactivate the tumor-suppressor function of pRb (reviewed by McIntyre et al., 1993). Functional relevance of alterations at the Rb-binding domain in the E7 gene remains unclear.In this context, we established 12 new uterine cervical-cancer cell lines (SNU-17, SNU-487, SNU-523, SNU-682, SNU-703, SNU-778, SNU-902, SNU-1000, SNU-1005, SNU-1160, SNU-1245 and SNU-1299) with early passages and described cell phenotypes, including histopathology of the primary tumor and in vitro growth characteristics. We also performed molecular characterization, including DNA-fingerprinting analysis and HPV detection, typing and determination of physical form (integrated or episomal) of viral DNA. RNA expression of HPV E6/E7 viral transcripts, sequence variation in the E7 gene of HPV and genetic alteration of p53 were also examined. MATERIAL AND METHODS Cell-line establishment and maintenanceCell lines were established from pathologically proven uterine cervical carcinomas (Table I). Solid tumors were finely minced with scissors and dissociated into small aggregates by pipetting. Appropriate amounts...

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Geographical dependence of sequence variation in the E7 gene of human papillomavirus type 16

Cited by 57 publications

References 14 publications

Human Papillomavirus Type 16 Variant Analysis of E6, E7, and L1 Genes and Long Control Region in Identification of Cervical Carcinomas in Patients in Northeast China

Human Papillomavirus Type 16 Variant Analysis of E6, E7, and L1 Genes and Long Control Region in Identification of Cervical Carcinomas in Patients in Northeast China

Sequence variations and viral genomic state of human papillomavirus type 16 in penile carcinomas from Ugandan patients.

Establishment and characterization of 12 uterine cervical-carcinoma cell lines: Common sequence variation in theE7 gene of HPV-16-positive cell lines

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