South American fire ant decapitating flies in the genus
Pseudacteon
(Diptera: Phoridae) are potential biocontrol agents of the invasive fire ants
Solenopsis invicta
and
S
.
richteri
in the United States and other regions of the world due to their high host specificity and the direct and indirect damage to their host ants. Despite their importance and the fact that several flies have already been released in the US, little is known about the genetic variability and phylogenetic relationships of
Pseudacteon
flies parasitizing South American fire ants in the
Solenopsis saevissima
species-group. A species delimitation analysis was conducted using a distance-based method (ABGD) and two tree-based methods (GMYC and mPTP) using COI sequences of 103 specimens belonging to 20 of the 22
Pseudacteon
species known from southern South America. Additionally, phylogenetic relationships between the already described and new candidate species were inferred using mitochondrial (COI) and nuclear (
wingless
) sequences. The species delimitation analysis suggests that species richness in these flies has been previously underestimated, due to the existence of putative cryptic species within nominal
Pseudacteon obtusus
,
P
.
pradei
,
P
.
tricuspis
,
P
.
cultellatus
, and
P
.
nudicornis
. Geographic distribution and host fire ant species seem to support cryptic lineages, though additional morphological data are needed to corroborate these results. All phylogenetic analyses reveal that South American fire ant decapitating flies are grouped into two main clades, with
Pseudacteon convexicauda
sister and well differentiated relative to these clades. Neither host nor geographic association appeared to be related to the differentiation of these two main clades within South American fire ant decapitating flies. This work provides information that will allow testing whether the putative cryptic phorid fly species show differences in their effectiveness as biocontrol agents against the highly invasive imported fire ants.