Geometry of Neonatal and Adult Red Blood Cells

Linderkamp, Otwin; Wu, Paul Y K; Meiselman, Herbert J.

doi:10.1203/00006450-198304000-00003

Cited by 62 publications

(35 citation statements)

References 14 publications

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“…Our results therefore suggest that the decreased deformability of fetal red cells is mainly due to their increased volume. We found a 24% increase in volume, which is in agreement with Linderkamp et al (20). The minimum cylindrical volume of fetal red cells, i.e.…”

Section: Discussionsupporting

confidence: 82%

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Rheology of Fetal and Maternal Blood

et al. 1985

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Section: Discussionsupporting

confidence: 82%

“…The minimum cylindrical volume of fetal red cells, i.e. the thinnest cylinder through which a red cell can pass, was reported to be 8% higher than for maternal RBCs (20), others found it to be normal (6). Recently, Linderkamp et ul.…”

Section: Discussionmentioning

confidence: 99%

Rheology of Fetal and Maternal Blood

et al. 1985

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“…These results are not contradictory to the reports of decreased filterability of neonatal RBC (13)(14)(15)(16) because the impaired filterability may be due to small subpopulations of poorly deformable (Fig. 3) and of very large RBC (10,21) in neonatal blood. These cells could obstruct filter pores, thereby precluding the passage of subsequent RBC.…”

Section: Discussioncontrasting

confidence: 56%

Erythrocyte Deformability in the Fetus, Preterm, and Term Neonate

et al. 1986

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ABSTRACT. Deformability of red blood cells (RBC) is an important determinant of microcirculation, of oxygen transport and release to the tissues, and of RBC life span. Deformability of RBC from five fetuses, 20 preterm infants, 20 term neonates, and 20 adults was determined by direct microscopic observation of RBC subjected to shear stresses of 6 to 85 dyn/cm2 using a counter-rotating rheoscope. There was no significant difference in deformability among RBC from the fetuses, the preterm and term neonates, and the adults at any shear stress. More than 95% of fetal, neonatal, and adult RBC were capable of tanktread motion. Compared to adults, the frequency distribution of RBC deformability was slightly broader in the fetuses and neonates because of the presence of more highly and poorly deformable RBC. The increased number of rigid RBC may contribute to the shortened life span of fetal RBC. (Pediatr Res 20:93-96, 1986) Abbreviation RBC, red blood cell Studies on the deformability of RBC from full-term neonates gave conflicting results: neonatal and adult RBC show no difference in deformability when studied under defined shear forces by a rheoscope (10) or an ektacytometer (1 1). However, RBC from term neonates require higher suction pressures for complete aspiration into narrow micropipettes (12) and are less filterable (1 3-16) than adult RBC.Little is known about the deformability of RBC from preterm infants. Buchan (15) studied filterability of RBC from preterm infants with gestational age of 34 to 37 wk and measured lower filtration rates in preterm infants compared to term neonates. Coulombel et al.(1 1) used an ektacytometer to determine deformability of RBC from 1 I neonates with 35 to 40 wk of gestational age. They did not find a correlation between RBC deformability and gestational age. Studies on the deformability of RBC from fetuses and small preterm infants do not appear to exist in the literature.In the present study, deformability of RBC from fetuses with 18 to 20 wk and from preterm and term neonates with 24 to 4 1 wk of gestational age were measured by means of a rheoscope. Our results indicate no significant difference in deformability between RBC from fetuses, preterm infants, term neonates, and adults.For the premature infant, maintenance of an adequate tissue MATERIALS AND METHODS oxygen supply is a major problem. Oxygen transport to the tissues is dependent on effective blood flow and on rapid oxygenThe deformability measurements were performed on blood release from the RBC. Both effective blood flow and the rate of samples from five fetuses (18-20 wk of gestation), 20 preterm oxygen uptake are influenced by the ability of RBC to deform infants, 20 term neonates, and 20 adults. The study was approved (i.e. to assume new shapes) (1-3). RBC deformability is essential by the Department of Pediatrics Human Subjects Research Comat four levels: 1) adaptation to higher shear forces in large vessels mittee. Informed consent was obtained from the mothers of the with rapid flow, thereby lowering bloo...

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“…Nevertheless, we used 18 degrees of freedom for both tests because the number of degrees of freedom should be based on the number of samples rather than on the total number of observations because of possible interindividual differences (27). 18 degrees of freedom). The significance was high (P < 0.001) at each shear stress and in each of the two groups independent of whether a two-tailed paired t test (differences between the 10 sample means) or a two-tailed unpaired t test (comparison of the means and S.D.…”

Section: Resultsmentioning

confidence: 99%

“…Filterability of RBC is influenced by cell geometry (3,31). Neonatal RBC have the same excess surface area and shape as adult cells; however, the minimal cylindrical diameter (i.e., the minimum diameter tube which a cell can enter without loss of volume) of neonatal RBC is larger than that for adult RBC (18). The impaired filterability of neonatal RBC may be the result of specific differences in cell geometry.…”

Section: Discussionmentioning

confidence: 99%

Deformability of Density Separated Red Blood Cells in Normal Newborn Infants and Adults

Linderkamp

Meiselman

1982

Pediatr Res

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SummaryThe deformability of neonatal and adult red blood cells (RBC) was studied using both unseparated and density (i.e., age) fractionated RBC. Blood samples were obtained from to normal newborn infants (placental blood) and to adults. Deformability was measured by direct microscopic observation of RBC subjected to shear stresses of 2.5-500 dynes/cm 2 using a counter-rotating Rheoscope. There was no significant difference in deformability between unseparated neonatal and adult RBC at any shear stress. The 3% least dense ("youngest") RBC showed significantly decreased hemoglobin concentrations (MCHC), increased volumes (MCV) and elevated deformability at any shear stress when compared to the 3% most dense ("oldest") cells, with the values for the unseparated RBC lying between these two extremes. The least dense neonatal RBC had a significantly lower MCHC and higher deformability than the least dense adult cells; however, the most dense neonatal RBC had lost more volume than the adult cells (33 versus 17%), had a higher MCHC, and were less deformable than the most dense adult cells. This indicates that the accelerated decrease in deformability of aging neonatal RBC is related to the more pronounced increase in MCHC, which is the principal determinant of the internal viscosity of the RBC Speculation The shortened survival time of neonatal red blood cells (RBC) is probably the consequence of accelerated aging of neonatalRBC. Differences in biochemical composition and a decrease in some RBC activities, which are important for the maintenance of membrane integrity, cell volume, and deformability might be responsible for the faster loss of membrane fragments and deformability of neonatal RBC when compared to adult cells. These alterations presumably impede the ability of aging neonatal RBC to pass the narrow splenic slits, thus promoting their eventual destruction by macrophages.

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Geometry of Neonatal and Adult Red Blood Cells

Cited by 62 publications

References 14 publications

Rheology of Fetal and Maternal Blood

Rheology of Fetal and Maternal Blood

Erythrocyte Deformability in the Fetus, Preterm, and Term Neonate

Deformability of Density Separated Red Blood Cells in Normal Newborn Infants and Adults

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