The aim of this study was to determine and compare some morphometric characteristics of Microtus species occurring in the central Anatolia, Turkey. This study is based on 209 specimens of Microtus spp. collected the central Anatolia between 2003 and 2010. Some features concerning pelage coloration, cranial characters, tooth morphotype, and baculum morphology of the species were recorded to determine and evaluate their taxonomic characteristics. It was determined that four species of the genus Microtus occur in the study area, including Microtus dogramacii, Microtus guentheri, Microtus hartingi, and Microtus mystacinus. Microtus hartingi has been found to be widespread throughout central Anatolia. Microtus guentheri is located in the type locality and nearby provinces. M. guentheri and M. hartingi were not found to be sympatric. Among species, M. hartingi has the longest hind foot and M. mystacinus has the longest tail. The UPGMA trees were constructed for each sex, using skull and external measurements of Microtus specimens. As a result, M. dogramacii and M. guentheri being the most similar, and M. mystacinus is the sister species to these. But, it was determined that M. hartingi is distinctively from the these species.