Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia

Li, Wenjun; Xu, Ping; Schümann, Peter; Zhang, Yuqin; Pukall, Rüdiger; Xu, Li-Hua; Stackebrandt, Erko; Jiang, Cheng‐Lin

doi:10.1099/ijs.0.64749-0

Cited by 786 publications

(427 citation statements)

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“…Genomic DNA extraction, amplification and 16S rRNA gene sequencing were performed as described previously (Li et al 2007). Identification of phylogenetic neighbours and calculation of pairwise 16S rRNA gene sequence similarities were achieved using the EzTaxon server (http://www.eztaxon.org/) (Chun et al 2007).…”

Section: Preliminary Identification Of Actinobacteriamentioning

confidence: 99%

Isolation and characterization of culturable endophytic actinobacteria associated with Artemisia annua L.

Zhao

Huang

et al. 2011

Antonie van Leeuwenhoek

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Endophytic actinobacteria isolated from Artemisia annua were characterized and evaluated for their bioactivities. A total of 228 isolates representing at least 19 different genera of actinobacteria were obtained and several of them seemed to be novel taxa. An evaluation of antimicrobial activity showed that more isolates possessed activity towards plant pathogens than activity against other pathogenic bacteria or yeasts. High frequencies of PCR amplification were obtained for type I polyketide synthases (PKS-I, 21.1%), type II polyketide synthases (PKS-II, 45.2%) and nonribosomal peptide synthetases (NRPS, 32.5%). The results of herbicidal activity screening indicated that 19 out of 117 samples of fermentation broths completely inhibited the germination of Echinochloa crusgalli. This study indicated that endophytic actinobacteria associated with A. annua are abundant and have potentially beneficial and diverse bioactivities which should be pursued for their biotechnical promise.

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Section: Preliminary Identification Of Actinobacteriamentioning

confidence: 99%

Isolation and characterization of culturable endophytic actinobacteria associated with Artemisia annua L.

Zhao

Huang

et al. 2011

Antonie van Leeuwenhoek

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“…The 16S rRNA gene of the isolate was amplified by PCR as described previously (Li et al 2007). The obtained sequences were then aligned with related 16S rRNA sequences from GenBank data libraries.…”

Section: Identification Of the Strainmentioning

confidence: 99%

Anaerobic degradation of phenanthrene by a newly isolated humus-reducing bacterium, Pseudomonas aeruginosa strain PAH-1

Wang

et al. 2011

J Soils Sediments

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Purpose The anaerobic degradation of polycyclic aromatic hydrocarbons (PAHs) has great significance to PAHs' natural attenuation in contaminated sites. Previous studies mainly focused on anaerobic PAH degradation by mixed cultures with nitrate, sulfate, or Fe(III) oxides as electron acceptors, and the roles of pure cultures in the process was rarely reported. The aim of this paper is to isolate a pure culture that is capable of degrading phenanthrene anaerobically with anthraquinone-2,6-disulphonate (AQDS) as the sole electron acceptor and evaluate its environmental functions. Materials and methods A strain of pure culture was isolated from anodic solution of a microbial fuel cell via enrichment procedure with phenanthrene and AQDS under anaerobic conditions. Using the single colony isolation technique, a distinct colony was obtained and identified by the phenotypic and phylogenetic analysis. Its ability to reduce AQDS and degrade phenanthrene was conducted in serum bottles by standard anaerobic techniques (purged with 80% N 2 −20% CO 2 ). The concentration of AH 2 QDS and fructose was quantified by UV-vis spectrophotometer at 450 and 210 nm, respectively. Cells number was determined by direct plate counting on aerobic LB agar medium. The concentration of phenanthrene was determined using HPLC. Results and discussion Strain PAH-1 was identified as Pseudomonas aeruginosa. It could oxidize fructose or glucose to reduce AQDS and can support microbial growth by conserving energy from fructose to AQDS. It has the ability to degrade phenanthrene directly with AQDS as the sole electron acceptor (46.5% removal), and the microbial process may be AQDS dependent. The addition of small organic substances (fructose) could enhance the anaerobic biodegradation of phenanthrene from 46.5% to 56.7%. The anaerobic degradation of phenanthrene fits the pseudo-firstorder kinetics, giving the rate constants of 0.0233/day (R 2 =0.934) and 0.0328/day (R 2 =0.933) for non-fructose set and fructose set, respectively. Conclusions We successfully isolated a facultative anaerobe, P. aeruginosa strain PAH-1. This study was the first paper reporting that a pure culture (strain PAH-1) has the ability to anaerobically degrade phenanthrene with AQDS as the sole electron acceptor. The finding also explores the environmental significance of the Pseudomonas genus.

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“…Extraction of genomic DNA and PCR amplification of the 16S rRNA gene were performed as described by Li et al (2007). The sequence obtained was compared with available 16S rRNA gene sequences of cultured species using the EzTaxon-e server (http://eztaxon-e.ezbiocloud.net/; Kim et al, 2012).…”

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confidence: 99%

Tessaracoccus rhinocerotis sp. nov., isolated from the faeces of Rhinoceros unicornis

Chen

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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A novel Gram-stain-positive, non-spore-forming, irregular rod-shaped, non-motile and facultatively anaerobic actinobacterium, designated strain YIM 101269 T , was isolated from the faeces of Rhinoceros unicornis living in Yunnan Wild Animal Park, Yunnan province, south-west China. The isolate grew at 10-35 8C, at pH 6-12 and with 0-9 % (w/v) NaCl. The cell-wall peptidoglycan of the organism contained LL-diaminopimelic acid as the diagnostic diamino acid. The polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, three unidentified polar lipids, one unidentified aminophospholipid and three unknown glycolipids. The major cellar fatty acid was anteiso-C 15 : 0 .MK-10(H 4 ) was the predominant menaquinone. The DNA G+C content was 69.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain YIM 101269 T belonged to the genus Tessaracoccus, closely related to Tessaracoccus flavescens DSM 18582 T (97.4 % similarity). Based on the evidence from the present study, strain YIM 101269 T is considered to represent a novel species of the genus Tessaracoccus, for which the name Tessaracoccus rhinocerotis sp. nov. is proposed. The type strain is YIM 101269 T (5DSM 27579 T 5CCTCC AB 2013217 T ).The genus Tessaracoccus was erected by Maszenan et al. (1999) and was characterized as comprising facultatively aerobic, non-motile, oxidase-negative, catalase-positive, non-sporeforming, coccoid-shaped bacteria. Strain YIM 101269 T was isolated from the faeces of Rhinoceros unicornis living in Yunnan Wild Animal Park, Yunnan province, south-west China. Fresh faeces of Rhinoceros unicornis was collected into a sterile bag immediately after defecation and brought back to the lab. The samples were dried at 28 8C for 1 week, then heated at 80 8C for 1 h and ground lightly with a sterile pestle. The sample was dissolved with 18 ml of 0.1 % Na 4 P 2 O 7 and shaken for 1 h. Finally, the sample was disrupted for 40 s using an ultrasonic wave device. The ultrasonic wave machine used was 160 W and 50 KHz. Aliquots (100 ml) of the serial diluent of the samples were spread onto YIM 6 agar plates. The isolation medium (YIM 6) contained 1 % soluble starch, 0.03 % casein, 0.2 % KNO 3 , 0.2 % NaCl, 0.2 % KH 2 PO 4 , 0.002 % CaCO 3 , 0.005 % MgSO 4 . 7H 2 O, 0.001 % FeSO 4 . 7H 2 O and 1.3 % agar, pH 7.2, plus 50 mg K 2 Cr 2 O 7 l 21 . The plates were incubated at 28 8C for 10 days. The purified strain was routinely cultivated on YIM 38 agar plates (0.4 % glucose,0.4 % yeast extract, 0.5 % malt extract, 3.7 mg vitamin mixture, 1.3 % agar, pH 7.2) at 28 8C and stored as aqueous glycerol suspensions (20 %, v/v) at 220 8C.Morphological observations under a light microscope (model BH2; Olympus) and scanning electron microscope (Quanta 200, FEI) were observed following the procedure described by Cheng et al. (2013). Gram-staining was 3These authors contributed equally to this work.

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Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia

Cited by 786 publications

References 29 publications

Isolation and characterization of culturable endophytic actinobacteria associated with Artemisia annua L.

Isolation and characterization of culturable endophytic actinobacteria associated with Artemisia annua L.

Anaerobic degradation of phenanthrene by a newly isolated humus-reducing bacterium, Pseudomonas aeruginosa strain PAH-1

Tessaracoccus rhinocerotis sp. nov., isolated from the faeces of Rhinoceros unicornis

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