Geosmin-producing Species of Coelosphaerium (Synechococcales, Cyanobacteria) in Lake Shinji, Japan

Godo, Toshiyuki; Saki, Yukiko; Nojiri, Yukari; Tsujitani, M.; Sugahara, Shogo; Hayashi, Shinobu; Kamiya, Hiroshi; Ohtani, Shuji; Seike, Yasushi

doi:10.1038/srep41928

Cited by 22 publications

(14 citation statements)

References 36 publications

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“…The species of Cyanobacteria producing geosmin are diverse, belonging to several distinct taxons, namely, multicellular strains of Oscillatoriales, Nostocales and Synechococcales 10,11,23 and recently the unicellular Synechococcales Coelosphaerium sp. 13 , debunking the idea that only filamentous Cyanobacteria could produce geosmin 24,25 . Most of the knowledge on cyanobacterial producers is from freshwaters, probably due to the blooms related to odor and musty taste issues, while terrestrial, brackish and marine producers are rarely described or absent 11,15,24,25 .…”

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confidence: 99%

A novel cyanobacterial geosmin producer, revising GeoA distribution and dispersion patterns in Bacteria

Churro

Semedo-Aguiar

Silva

et al. 2020

Sci Rep

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cyanobacteria are ubiquitous organisms with a relevant contribution to primary production in all range of habitats. cyanobacteria are well known for their part in worldwide occurrence of aquatic blooms while producing a myriad of natural compounds, some with toxic potential, but others of high economical impact, as geosmin. We performed an environmental survey of cyanobacterial soil colonies to identify interesting metabolic pathways and adaptation strategies used by these microorganisms and isolated, sequenced and assembled the genome of a cyanobacterium that displayed a distinctive earthy/musty smell, typical of geosmin, confirmed by GC-MS analysis of the culture's volatile extract. Morphological studies pointed to a new Oscillatoriales soil ecotype confirmed by phylogenetic analysis, which we named Microcoleus asticus sp. nov. our studies of geosmin gene presence in Bacteria, revealed a scattered distribution among Cyanobacteria, Actinobacteria, Delta and Gammaproteobacteria, covering different niches. Careful analysis of the bacterial geosmin gene and gene tree suggests an ancient bacterial origin of the gene, that was probably successively lost in different time frames. The high sequence similarities in the cyanobacterial geosmin gene amidst freshwater and soil strains, reinforce the idea of an evolutionary history of geosmin, that is intimately connected to niche adaptation. Cyanobacteria can be found in marine, brackish and freshwaters exhibiting both planktonic and benthic lifestyles, covering almost all ranges of temperatures and salinities, in symbiosis with a variety of organisms, in soil, caves, deserts, and buildings, and are among the oldest life forms that evolved throughout time 1,2. It represents a prokaryotic group with huge diversity; in morphology presenting from unicellular to filamentous forms with specialized cells; chemo-diverse since they produce a panoply of bioactive compounds: antifungal 3,4 , anticancer 3,5 , antibacterial 3 , antiviral 5 , algicidal 6 , and antioxidant 7 , as well as toxic compounds noxious to the health of humans and wildlife 8,9. Two of the most common volatile organic compounds produced by Cyanobacteria are 2-Methylisoborneol (MIB, PubChem CID:16913) and Geosmin (PubChem CID:29746) 10-12. These compounds are frequently described in fresh and brackish waters Cyanobacteria, as they cause taste and odor problems in drinking water, fish and shellfish 11,13,14. Although non-toxic to humans, these compounds change the perception of safety by the public, causing major economic losses in both sectors 10,11,14. Geosmin is greatly associated with terrestrial environments. It is assumed that the organisms responsible for the gross production of geosmin in soil are Bacteria belonging to actinomycetes-Norcardia cummidelens; N. fluminea; Streptomyces Luridiscabiei; S. albidoflavus-since they are typical soil inhabitants 15,16. Other known producers of geosmin are fungi-Penicillium discolour, P. crustosum, P. expansum, Botrytis cinerea, and Chaetomium sp.-that are also present i...

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confidence: 99%

A novel cyanobacterial geosmin producer, revising GeoA distribution and dispersion patterns in Bacteria

Churro

Semedo-Aguiar

Silva

et al. 2020

Sci Rep

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“…Sphaerospermopsis, Cylindrospermopsis and Nodularia were investigated genera in this study which were already reported as GEO or MIB producer in previous studies (Popin et al 2016, Watson et al 2016, Zhang et al 2017, Pham et al 2020. Also, Microcystis strains were investigated with the knowledge of other coccoid cyanobacteria such as Synechococcus C.Nägeli or Coelosphaerium Nägeli as a producer (Kutovaya & Watson 2014, Godo et al 2017). On the other hand, the results are limited with the investigated strains and VOC production may vary from one strain to another (Watson et al 2016).…”

Section: Dolichospermummentioning

confidence: 99%

The First Report of Geosmin and 2-Methylisoborneol Producer Cyanobacteria From Turkish Freshwaters

Tunç

Akçaalan

Köker

et al. 2021

Trakya University Journal of Natural Sciences

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Water users consider the safety of water according to its aesthetic properties, primarily taste and odour. Geosmin (GEO) and 2-methylisoborneol (MIB) are the most common taste and odour compounds in freshwaters which cause an earthy and musty odour in water. Since human nose can detect these compounds in concentrations as low as 10 ng/L, it is essential to monitor drinking waters before consumer complaints and to produce a timely solution. Therefore, it is necessary to identify GEO and MIB producers to manage the problem at its source. Cyanobacteria are one of the main producers of these compounds in freshwater ecosystems. In this study, we analyzed 13 samples (9 cyanobacteria cultures from Bafa Lake, Elmalı Dam Lake, İznik Lake, Küçükçekmece Lake, Manyas Lake and Taşkısığı Lake, and 4 environmental water samples from Erfelek and Günpınar Waterfalls and Ömerli Dam Lake) for GEO and MIB production by HS-SPME (Head space-solid phase microextraction) coupled with GC-MS (gas chromatography-mass spectrometry). The presence of Cyanobacteriaspecific GEO and MIB synthase genes were also analyzed by PCR (Polymerase Chain Reaction). Taste and odour production was confirmed in 2 samples by GC-MS while 4 samples yielded positive results by PCR. All positive samples were environmental samples (3 samples from waterfalls from Günpınar and Erfelek Waterfalls, 1 sample from Ömerli Dam Lake -a drinking water reservoir) which were dominated by Nostoc Vaucher ex Bornet & Flahault, Phormidium Kützing ex Gomont and Pseudanabaena Lauterborn. This is the first report of GEO and MIB producing cyanobacteria in Turkish freshwaters by combining microscopy, analytical and molecular techniques.Özet: Su kullanıcıları, suyun güvenli olup olmadıklarına öncelikle onun tat ve kokusu gibi estetik özelliklerine bakarak karar vermektedir. Geosmin (GEO) ve 2-methylisoborneol (MIB), tatlısularda en yaygın olarak görülen tat ve koku bileşikleridir ve suyun toprak ve küf kokmasına neden olurlar. İnsanlar <10 ng/L gibi düşük konsantrasyonlarda dahi bu kokulara hassas olmalarından dolayı bu bileşiklerin içme sularında tüketici şikayetleri oluşmadan önce izlenmesi ve sorunun çözülmesi oldukça önemlidir. Bu sebeple, problemin kaynağında çözümlenebilmesi için GEO ve MIB üreticilerinin tespit edilmesi gereklidir. Tatlısu ekosistemlerinde bu bileşiklerin başlıca üreticilerinden biri siyanobakterilerdir (Cyanobacteria). Bu çalışmada 13 örnek (9 siyanobakteri kültürü, Bafa Gölü, Elmalı Baraj Gölü, İznik Gölü, Küçükçekmece Gölü, Manyas Gölü, Taşkısığı Gölü'nden ve 4 çevresel su örneği, Günpınar, Erfelek şelaleleri ve Ömerli Baraj Gölü'nden) GEO ve MIB üretiminin tespiti için HS-SPME (Tepe Boşluğu-Katı Faz Mikro Ekstraksiyon) GC-MS (Gaz Kromatografi-Kütle Spektrometresi) yöntemi kullanılarak analiz edilmiştir. Ayrıca siyanobakterilere özgü GEO ve MIB sentaz genlerinin varlığının tespiti için PZR (Polimeraz Zincir Reaksiyonu) yöntemi kullanılmıştır. İki örnekte GC-MS ile tat ve koku üretimi tespit edilmiş ve 4 örnekte de PZR ile pozitif sonuç alınmıştır. Pozitif son...

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“…The molecular weight (MW) and isoelectric point (pI) of each predicted MaVPE protein sequence were calculated using ExPASy 5 . Multiple sequence alignment analysis of MaVPE proteins were performed using the ClustalW logarithm (Godo et al, 2017). Further processing of the alignment files was conducted in the BioEdit sequence alignment editor (version 7.1.3.0) with default parameter settings.…”

Section: In Silico and Phylogenetic Analysismentioning

confidence: 99%

Vacuolar Processing Enzymes Modulating Susceptibility Response to Fusarium oxysporum f. sp. cubense Tropical Race 4 Infections in Banana

et al. 2022

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Fusarium oxysporum f. sp. cubense tropical race 4 (FocTR4) is a destructive necrotrophic fungal pathogen afflicting global banana production. Infection process involves the activation of programmed cell death (PCD). In this study, seven Musa acuminata vacuolar processing enzyme (MaVPE1–MaVPE7) genes associated with PCD were successfully identified. Phylogenetic analysis and tissue-specific expression categorized these MaVPEs into the seed and vegetative types. FocTR4 infection induced the majority of MaVPE expressions in the susceptible cultivar “Berangan” as compared to the resistant cultivar “Jari Buaya.” Consistently, upon FocTR4 infection, high caspase-1 activity was detected in the susceptible cultivar, while low level of caspase-1 activity was recorded in the resistant cultivar. Furthermore, inhibition of MaVPE activities via caspase-1 inhibitor in the susceptible cultivar reduced tonoplast rupture, decreased lesion formation, and enhanced stress tolerance against FocTR4 infection. Additionally, the Arabidopsis VPE-null mutant exhibited higher tolerance to FocTR4 infection, indicated by reduced sporulation rate, low levels of H2O2 content, and high levels of cell viability. Comparative proteomic profiling analysis revealed increase in the abundance of cysteine proteinase in the inoculated susceptible cultivar, as opposed to cysteine proteinase inhibitors in the resistant cultivar. In conclusion, the increase in vacuolar processing enzyme (VPE)-mediated PCD played a crucial role in modulating susceptibility response during compatible interaction, which facilitated FocTR4 colonization in the host.

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Geosmin-producing Species of Coelosphaerium (Synechococcales, Cyanobacteria) in Lake Shinji, Japan

Cited by 22 publications

References 36 publications

A novel cyanobacterial geosmin producer, revising GeoA distribution and dispersion patterns in Bacteria

A novel cyanobacterial geosmin producer, revising GeoA distribution and dispersion patterns in Bacteria

The First Report of Geosmin and 2-Methylisoborneol Producer Cyanobacteria From Turkish Freshwaters

Vacuolar Processing Enzymes Modulating Susceptibility Response to Fusarium oxysporum f. sp. cubense Tropical Race 4 Infections in Banana

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