Germ Cell Lineage from a Single Blastomere at 8-Cell Stage in Shiro-uo (ice goby)

Saito, Taiju; Otani, Satoshi; Nagai, Torao; Nakatsuji, Takako; Arai, Katsutoshi; Yamaha, Etsuro

doi:10.2108/zsj.19.1027

Cited by 9 publications

(12 citation statements)

References 29 publications

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“…5G, N). The migration route of the bright cells is similar to the dynamics of PGCs observed by vasa gene expression (data not shown) and that reported for PGCs in a histological analysis of ice goby embryos (Saito et al, 2002).…”

Section: The Numbers Of Bright Cells Seen In Embryos Of Different Fissupporting

confidence: 84%

“…The migration route of these bright cells in goldfish, zebrafish, medaka, loach and ice goby were identical to those of PGCs in each species Yoon et al, 1997;Tanaka et al, 2001;Saito et al, 2002;and author's unpublished data). In herring, the final localized position of bright cells was identical to that found for PGCs by histological observation, suggesting that the bright cells are PGCs.…”

Section: Identification Of Bright Cells By Mrna Injectionmentioning

confidence: 67%

“…It is likely that the yolk ball and its covering mesoendodermal germ layer provide PGCs with favorable fields of signals. Indeed, in many fish species, positional closeness between PGCs and the yolk syncytial layer (YSL) and endodermal cells have been observed during migration (goldfish - Kazama-Wakabayashi et al, 1999;zebrafish -Nagai et al, 2001; ice goby and floating goby - Saito et al, 2002. Therefore, more studies will be required to identify molecular factors that control PGC migration and to correlate these with associated histological observations.…”

Section: Migration Route Of Pgcsmentioning

confidence: 99%

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Visualization of primordial germ cells in vivo using GFP-nos1 3'UTR mRNA

Saito¹,

Fujimoto²,

Maegawa³

et al. 2006

Int. J. Dev. Biol.

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124

109

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In some teleost fish, primordial germ cells (PGCs) inherit specific maternal cytoplasmic factors such as vasa and nanos 1 (nos1) mRNA. It has been shown that the 3'untranslated regions (UTRs) of vasa and nos1 have critical roles for stabilization of these RNAs in zebrafish PGCs. In this study, to determine whether this role of the nos 1 3'UTR is conserved between teleost species, we injected artificially synthesized mRNA, combining green fluorescent protein (GFP) and the zebrafish nos 1 3'UTR (GFP-nos 1 3'UTR mRNA), into the fertilized eggs of various fish species. The 3'UTR of the Oryzias latipes vasa homologue (olvas ) mRNA was assayed in the same manner. We demonstrate that the PGCs of seven teleost species could be visualized using GFP-nos 1 3'UTR mRNA. GFP-olvas 3'UTR mRNA did not identify PGCs in herring or loach embryos, but did enable visualization of the PGCs in medaka embryos. Our results indicate that the 3'UTR of the zebrafish nos1 mRNA can promote maintenance of RNAs in the PGCs of different fish species. Finally, we describe and compare the migration routes of PGCs in seven teleost species.

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Section: The Numbers Of Bright Cells Seen In Embryos Of Different Fissupporting

confidence: 84%

Section: Identification Of Bright Cells By Mrna Injectionmentioning

confidence: 67%

Section: Migration Route Of Pgcsmentioning

confidence: 99%

See 1 more Smart Citation

Visualization of primordial germ cells in vivo using GFP-nos1 3'UTR mRNA

Saito¹,

Fujimoto²,

Maegawa³

et al. 2006

Int. J. Dev. Biol.

Self Cite

124

109

View full text Add to dashboard Cite

show abstract

“…Thereafter, PGCs form broad aggregations around the anterior part of yolk extension, and finally settle in the upper part of body cavity, via the surface of the gut. The aggregation of PGCs under the body axis is also observed histologically in ice goby (Saito et al, 2002). In goldfish, PGCs also align in a similar manner, as observed in ukigori, but never move underneath the body before gut formation .…”

Section: Migration Of Pgcssupporting

confidence: 54%

“…When the blastoderm was removed and transplanted onto the other blastula, from which the upper part of blastoderm was removed, ectopic PGCs around the head region increased in number Otani et al, 2005). Therefore, ectopic PGCs around the head region are explained by the distribution of its founder around the animal part of the blastoderm at the phase from the blastula to gastrula stage by the heavy mixing of the blastomere during these stages Saito 2002). …”

Section: Migration Of Pgcsmentioning

confidence: 99%

Developmental biotechnology for aquaculture, with special reference to surrogate production in teleost fishes

Yamaha

Saito

Goto-Kazeto

et al. 2007

Journal of Sea Research

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The purpose of this review is to introduce surrogate production as a new technique for fish-seed production in aquaculture. Surrogate production in fish is a technique used to obtain the gametes of a certain genotype through the gonad of another genotype. It is achieved by inducing germ-line chimerism between different species during early development. Primordial germ cells (PGCs) are a key material of this technique to induce germ-line chimera. In several species, it has been reported that PGCs differentiated from the blastomeres inherited some maternally supplied mRNA located at the terminal regions of the early cleavage furrows. PGCs from donor species (or strains) are isolated and transplanted into host species to induce the germ-line chimera.Four methods for inducing germ-line chimera, namely, blastomere transplantation, blastoderm-graft transplantation, transplantation of PGC from the genital ridge and transplantation visualized PGC with GFP fluorescence, are described. Several problems preventing the successful induction of germ-line chimera in various fish species are mentioned. Surrogate production, however, has the possibility to allow efficient fish-seed production and effective breeding and transfer of biodiversity to an aquaculture strain.Conservation and efficient utilization of genetic resources will be achieved through surrogate production combined with the cryopreservation of PGCs.

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Fish on glass. The science and art of histology

Cole

2012

Environ Biol Fish

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Germ Cell Lineage from a Single Blastomere at 8-Cell Stage in Shiro-uo (ice goby)

Cited by 9 publications

References 29 publications

Visualization of primordial germ cells in vivo using GFP-nos1 3'UTR mRNA

Visualization of primordial germ cells in vivo using GFP-nos1 3'UTR mRNA

Developmental biotechnology for aquaculture, with special reference to surrogate production in teleost fishes

Fish on glass. The science and art of histology

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