Germ cell sex prior to meiosis in the rainbow trout

Li, Mingyou; Shen, Qian; Wong, Foong Mei; Xu, Hongyan; Hóng, Ní; Zeng, Lingbing; Liu, Lin; Wei, Qiwei; Hong, Yunhan

doi:10.1007/s13238-011-1003-8

Cited by 10 publications

(11 citation statements)

References 30 publications

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“…In the fish species examined in detail by in situ hybridization (ISH), vasa expression occurs in both mitotic and meiotic germ cells of both sexes 27 , 32 . A similar observation has been described also for other germ genes such as boule and dazl in medaka 32 and dazl in trout 9 . One exception is boule , whose expression takes place in male gonia but not in female gonia 9 .…”

Section: Introductionsupporting

confidence: 86%

“…This distinction is usually made on the basis of difference of DAPI staining between mitotic oogonia and meiotic oocytes. This dye does not stain the oocyte nucleus in many teleost species including medaka 32 and trout 9, 39. Another dye is propidium iodide, which produces a similarly differential staining in zebrafish 29, gibel carp 27.…”

Section: Discussionmentioning

confidence: 99%

“…A conserved interplay between germ cells and gonadal sex has indeed been reported in cattle, zebrafish and medaka 8 , where animals with germ cells usually develop into two opposite sexes, while those without germ cells produce only one sex, male. We have recently revealed differential expression of germ genes boule and dazl in male and female gonial cells of the rainbow trout, pointing to germ cell sex prior to meiosis and possibly a role for germ cells in sex differentiation 9 , 10 .…”

Section: Introductionmentioning

confidence: 98%

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Vasa Identifies Germ Cells and Critical Stages of Oogenesis in the Asian Seabass

Xu¹,

Lim²,

Dwarakanath³

et al. 2014

Int. J. Biol. Sci.

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Germ cells produce sperm and eggs for reproduction and fertility. The Asian seabass (Lates calcarifer), a protandrous marine fish, undergoes male-female sex reversal and thus offers an excellent model to study the role of germ cells in sex differentiation and sex reversal. Here we report the cloning and expression of vasa as a first germ cell marker in this organism. A 2241-bp cDNA was cloned by PCR using degenerate primers of conserved sequences and gene-specific primers. This cDNA contains a polyadenylation signal and a full open reading frame for 645 amino acid residues, which was designated as Lcvasa for the seabass vasa, as its predicted protein is homologous to Vasa proteins. The Lcvasa RNA is maternally supplied and specific to gonads in adulthood. By chromogenic and fluorescent in situ hybridization we revealed germ cell-specific Lcvasa expression in both the testis and ovary. Importantly, Lcvasa shows dynamic patterns of temporospatial expression and subcellular distribution during gametogenesis. At different stages of oogenesis, for example, Lcvasa undergoes nuclear-cytoplasmic redistribution and becomes concentrated preferentially in the Balbiani body of stage-II~III oocytes. Thus, the vasa RNA identifies both female and male germ cells in the Asian seabass, and its expression and distribution delineate critical stages of gametogenesis.

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Section: Introductionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Vasa Identifies Germ Cells and Critical Stages of Oogenesis in the Asian Seabass

Xu¹,

Lim²,

Dwarakanath³

et al. 2014

Int. J. Biol. Sci.

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“…Boule is the only DAZ family member in invertebrates including Drosophila 20 and C. elegans 21. In the rainbow trout, differential expression of boule and dazl reveals germ cell sex prior to meiosis2223.…”

mentioning

confidence: 99%

Dazl is a critical player for primordial germ cell formation in medaka

Zhu

et al. 2016

Sci Rep

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The DAZ family genes boule, daz and dazl have conserved functions in primordial germ cell (PGC) migration, germ stem cell proliferation, differentiation and meiosis progression. It has remained unknown whether this family is required for PGC formation in developing embryos. Our recent study in the fish medaka (Oryzias latipes) has defined dnd as the critical PGC specifier and predicted the presence of additional factors essential for PGC formation. Here we report that dazl is a second key player for medaka PGC formation. Dazl knockdown did not prevent PGC formation even in the absence of normal somatic structures. It turned out that a high level of Dazl protein was maternally supplied and persisted until gastrulation, and hardly affected by two antisense morpholino oligos targeting the dazl RNA translation. Importantly, microinjection of a Dazl antibody remarkably reduced the number of PGCs and even completely abolished PGC formation without causing detectable somatic abnormality. Therefore, medaka PGC formation requires the Dazl protein as maternal germ plasm component, offering first evidence that dazl is a critical player in PGC formation in vivo. Our results demonstrate that antibody neutralization is a powerful tool to study the roles of maternal protein factors in PGC development in vivo.

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“…The samples were finally stained for nuclei by using DAPI (µg/ml) and embedded in the Gold Antifade reagent (Invitrogen) for microscopy. In this FISH procedure, two differently labeled antisense RNA probes are co-incubated with a sample in the hybridization step and subjected to sequential color development into green and red fluorescence 62.…”

Section: Methodsmentioning

confidence: 99%

Differential Evolution of Duplicated Medakafish mitf Genes

Li¹,

Zhu²,

Hong³

2013

Int. J. Biol. Sci.

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Gene duplication is a major force of evolution. One whole genome duplication (WGD) event in the fish ancestor generated genome-wide duplicates in all modern species. Coloration and patterning on the animal body surface exhibit enormous diversity, representing a mysterious and ideal system for understanding gene evolution. Surface colors and patterns are determined primarily by pigment cells in the skin and eye. Thus, microphthalmia-associated transcription factor (Mitf) as a master regulator of melanocyte development is excellent for studying the evolution of WGD-derived gene duplicates. Here we report the evolution of mitf duplicate, mitf1 and mitf2, in the fish medaka (Oryzias latipes), which encode medaka co-homologs Mitf1 and Mitf2 of the mouse Mitf. Compared to mitf1, mitf2 exhibits an accelerated sequence divergence and loses melanocytic expression in embryos at critical developmental stages. Compared to a Xiphophorus counterpart, the medaka Mitf2 displayed a reduced activity in activating melanogenic gene expression by reporter assays and RT-PCR analyses. We show that the medaka Mitf2 has the ability to induce melanocyte differentiation in medaka embryonic stem cells but at a remarkably reduced efficiency compared to the Xiphophorus counterpart. Our data suggest differential evolution of the medaka mitf duplicate, with mitf1 adopting conservation and mitf2 employing degeneration, which is different from the duplication-degeneration-complementation proposed as the mechanism to preserve many gene duplicates in zebrafish. Our finding reveals species-specific variations for mitf duplicate evolution, in agreement with enormous diversity of body coloration and patterning.

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Germ cell sex prior to meiosis in the rainbow trout

Cited by 10 publications

References 30 publications

Vasa Identifies Germ Cells and Critical Stages of Oogenesis in the Asian Seabass

Vasa Identifies Germ Cells and Critical Stages of Oogenesis in the Asian Seabass

Dazl is a critical player for primordial germ cell formation in medaka

Differential Evolution of Duplicated Medakafish mitf Genes

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