Germ-cell specific eIF4E1B regulates maternal RNA translation 
to ensure zygotic genome activation

Yang, Guanghui; Xin, Qiliang; Feng, Iris; Dean, Jurrien

doi:10.21203/rs.3.rs-1901015/v1

Cited by 2 publications

(1 citation statement)

References 68 publications

(86 reference statements)

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“…Regulates maternal mRNA translation [66] magoh A component of exon-exon junction complex [67] tdrd5 Binds piRNA and is required for transposon silencing [68] https://doi.org/10.1371/journal.pgen.1010589.t005…”

Section: Gene Known Functions Reference Fkbp6mentioning

confidence: 99%

The RNA-binding protein Adad1 is necessary for germ cell maintenance and meiosis in zebrafish

et al. 2023

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The double stranded RNA binding protein Adad1 (adenosine deaminase domain containing 1) is a member of the adenosine deaminase acting on RNAs (Adar) protein family with germ cell-specific expression. In mice, Adad1 is necessary for sperm differentiation, however its function outside of mammals has not been investigated. Here, through an N-ethyl-N-nitrosourea (ENU) based forward genetic screen, we identified an adad1 mutant zebrafish line that develops as sterile males. Further histological examination revealed complete lack of germ cells in adult mutant fish, however germ cells populated the gonad, proliferated, and entered meiosis in larval and juvenile fish. Although meiosis was initiated in adad1 mutant testes, the spermatocytes failed to progress beyond the zygotene stage. Thus, Adad1 is essential for meiosis and germline maintenance in zebrafish. We tested if spermatogonial stem cells were affected using nanos2 RNA FISH and a label retaining cell (LRC) assay, and found that the mutant testes had fewer LRCs and nanos2-expressing cells compared to wild-type siblings, suggesting that failure to maintain the spermatogonial stem cells resulted in germ cell loss by adulthood. To identify potential molecular processes regulated by Adad1, we sequenced bulk mRNA from mutants and wild-type testes and found mis-regulation of genes involved in RNA stability and modification, pointing to a potential broader role in post-transcriptional regulation. Our findings suggest that the RNA regulatory protein Adad1 is required for fertility through regulation of spermatogonial stem cell maintenance in zebrafish.

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Section: Gene Known Functions Reference Fkbp6mentioning

confidence: 99%

The RNA-binding protein Adad1 is necessary for germ cell maintenance and meiosis in zebrafish

et al. 2023

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Human Tissues Exhibit Diverse Composition of Translation Machinery

Anisimova

Kolyupanova

Makarova

et al. 2023

IJMS

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While protein synthesis is vital for the majority of cell types of the human body, diversely differentiated cells require specific translation regulation. This suggests the specialization of translation machinery across tissues and organs. Using transcriptomic data from GTEx, FANTOM, and Gene Atlas, we systematically explored the abundance of transcripts encoding translation factors and aminoacyl-tRNA synthetases (ARSases) in human tissues. We revised a few known and identified several novel translation-related genes exhibiting strict tissue-specific expression. The proteins they encode include eEF1A1, eEF1A2, PABPC1L, PABPC3, eIF1B, eIF4E1B, eIF4ENIF1, and eIF5AL1. Furthermore, our analysis revealed a pervasive tissue-specific relative abundance of translation machinery components (e.g., PABP and eRF3 paralogs, eIF2B and eIF3 subunits, eIF5MPs, and some ARSases), suggesting presumptive variance in the composition of translation initiation, elongation, and termination complexes. These conclusions were largely confirmed by the analysis of proteomic data. Finally, we paid attention to sexual dimorphism in the repertoire of translation factors encoded in sex chromosomes (eIF1A, eIF2γ, and DDX3), and identified the testis and brain as organs with the most diverged expression of translation-associated genes.

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Germ-cell specific eIF4E1B regulates maternal RNA translation to ensure zygotic genome activation

Cited by 2 publications

References 68 publications

The RNA-binding protein Adad1 is necessary for germ cell maintenance and meiosis in zebrafish

The RNA-binding protein Adad1 is necessary for germ cell maintenance and meiosis in zebrafish

Human Tissues Exhibit Diverse Composition of Translation Machinery

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