Germ cells in the crustacean Parhyale hawaiensis depend on Vasa protein for their maintenance but not for their formation

Özhan, Günes; Havemann, Jo; Gerberding, Matthias

doi:10.1016/j.ydbio.2008.10.028

Cited by 63 publications

(62 citation statements)

References 49 publications

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“…These genes are also expressed in broad regions at early stages of development in multiple species across animal phylogeny including flatworms, mollusks, annelids and arthropods and involvement of these factors in embryogenesis has been proposed. 38,[43][44][45][46][47][48][49][50] The highly similar expression domains of Nematostella piwi and vasa homologs (Fig. 3) are in line with their clustering in the nCounter analysis (Fig.…”

Section: Discussionsupporting

confidence: 74%

Characterization of the piRNA pathway during development of the sea anemone Nematostella vectensis

et al. 2017

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PIWI-interacting RNAs (piRNAs) and associated proteins comprise a conserved pathway for silencing transposons in metazoan germlines. piRNA pathway components are also expressed in multipotent somatic stem cells in various organisms. piRNA functions have been extensively explored in bilaterian model systems, however, comprehensive studies in non-bilaterian phyla remain limited. Here we investigate the piRNA pathway during the development of Nematostella vectensis, a well-established model system belonging to Cnidaria, the sister group to Bilateria. To date, no population of somatic stem cells has been identified in this organism, despite its long life-span and regenerative capacities that require a constant cell-renewal. We show that Nematostella piRNA pathway components are broadly expressed in early developmental stages, while piRNAs themselves show differential expression, suggesting specific developmental roles of distinct piRNA families. In adults, piRNA associated proteins are enriched in the germline but also expressed in somatic cells, indicating putative stem cell properties. Furthermore, we provide experimental evidence that Nematostella piRNAs cleave transposable elements as well as protein-coding genes. Our results demonstrate that somatic expression of piRNA associated proteins as well as the roles of piRNAs in transposon repression and gene regulation are likely ancestral features that evolved before the split between Cnidaria and Bilateria.

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Section: Discussionsupporting

confidence: 74%

Characterization of the piRNA pathway during development of the sea anemone Nematostella vectensis

et al. 2017

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“…1). As a proof of principle, we demonstrate iTRAC in Parhyale hawaiensis, a crustacean that has emerged as an attractive model for developmental studies (Browne et al, 2005;Extavour, 2005;Gerberding et al, 2002;Liubicich et al, 2009;Ozhan-Kizil et al, 2008;Pavlopoulos and Averof, 2005;Pavlopoulos et al, 2009;Price et al, 2010;Rehm et al, 2009;Vargas-Vila et al, 2010).…”

Section: Introductionmentioning

confidence: 89%

A versatile strategy for gene trapping and trap conversion in emerging model organisms

et al. 2011

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SUMMARYGenetic model organisms such as Drosophila, C. elegans and the mouse provide formidable tools for studying mechanisms of development, physiology and behaviour. Established models alone, however, allow us to survey only a tiny fraction of the morphological and functional diversity present in the animal kingdom. Here, we present iTRAC, a versatile gene-trapping approach that combines the implementation of unbiased genetic screens with the generation of sophisticated genetic tools both in established and emerging model organisms. The approach utilises an exon-trapping transposon vector that carries an integrase docking site, allowing the targeted integration of new constructs into trapped loci. We provide proof of principle for iTRAC in the emerging model crustacean Parhyale hawaiensis: we generate traps that allow specific developmental and physiological processes to be visualised in unparalleled detail, we show that trapped genes can be easily cloned from an unsequenced genome, and we demonstrate targeting of new constructs into a trapped locus. Using this approach, gene traps can serve as platforms for generating diverse reporters, drivers for tissue-specific expression, gene knockdown and other genetic tools not yet imagined.

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“… İletişim (Correspondence)  +90 264 2956818  cansua@sakarya.edu.tr embryonic development and they contain germ granules [2][3][4][5][6][7] . PGC migration must be finely regulated as it follows a complex pathway through various developing tissues [8] .…”

Section: Introductionmentioning

confidence: 99%

Effects of Low Doses of Bisphenol A on Primordial Germ Cells in Zebrafish (Danio rerio) Embryos and Larvae

Akbulut¹,

Kızıl²,

Yön³

2013

Kafkas Univ Vet Fak Derg

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SummaryPrimordial germ cells are the precursors of gametes in sexually reproducing organisms. The migration and homing process of primordial germ cells are prone to environmental effects and the endocrine system hormones. One of the environmental pollutants which humans are exposed to is bisfenol A (BPA). BPA which is an endocrine disrupter generally used for making plastics harder. In this study we investigated the effects of low doses of BPA (4 mg/L and 8 mg/L) on primordial germ cells at the zebrafish embryos and larvae. Whole mount in situ hybridization for germ cell marker gene vasa showed that upon exposure to BPA, the primordial germ cell number increased and these cells ectopically localized outside the normal germ cell niche in zebrafish. Migration of primordial germ cells into ectopic locations and morphological changes in these cells were also proved by histological studies. Additionally, results of acridine orange staining to detect apoptotic cells showed that low doses of BPA did not increase apoptosis. Results of the present study showed that low doses of BPA exposure increased the number of primordial germ cells and induced ectopic primordial germ cell localization in the zebrafish embryos. Keywords: Zebrafish, Primordial germ cell, Bisphenol A Düşük Dozlardaki Bisfenol A'nın Zebra Balığı (Danio rerio) Embriyo ve Larvalarında Primordiyal Germ Hücreleri Üzerine Etkisi ÖzetPrimordiyal germ hücreleri, eşeyli üreyen canlılarda gametlerin öncül hücreleridir. Endokrin sistem hormonları ve çevresel etkenler, primordiyal germ hücrelerinin göçünü etkilemektedir. İnsanların maruz kaldığı çevresel kirleticilerden biri de bisfenol A (BPA)'dır. Endokrin sistemde hasar oluşturan biri olan BPA, genellikle plastik sertleştirme işleminde kullanılmaktadır. Bu çalışmada zebra balığı embriyo ve larvalarında düşük dozlardaki (4 mg/L and 8 mg/L) BPA'nın etkileri incelenmiştir. Bir germ hücresi belirteci gen olan vasa ile yapılan whole mount in situ hibridizasyon deneyleri sonucunda BPA'nın primordiyal germ hücre sayısında artışa sebep olduğu ve bu hücrelerin göç yolu dışındaki (ektopik) bölgelerde bulunduğu tespit edilmiştir. Primordiyal germ hücrelerinin ektopik bölgelere göçü ve bu hücrelerdeki morfolojik değişiklikler histolojik çalışmalarla da doğrulanmıştır. Buna ek olarak, düşük dozlarıyla yapılan boyamalarda apoptozisin tespitinde kullanılan akridin turuncusu boyaması sonucunda BPA'nın apoptozisi artırmadığı saptanmıştır. Bu çalışma sonuçları, düşük dozlardaki BPA'nın primordiyal germ hücre sayısını artırdığını ve bu hücrelerin ektopik bölgelere göç etmesine yol açtığını ortaya koymaktadır.

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Germ cells in the crustacean Parhyale hawaiensis depend on Vasa protein for their maintenance but not for their formation

Cited by 63 publications

References 49 publications

Characterization of the piRNA pathway during development of the sea anemone Nematostella vectensis

Characterization of the piRNA pathway during development of the sea anemone Nematostella vectensis

A versatile strategy for gene trapping and trap conversion in emerging model organisms

Effects of Low Doses of Bisphenol A on Primordial Germ Cells in Zebrafish (Danio rerio) Embryos and Larvae

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