Germ tube induction in <i>Candida albicans</i>

Shepherd, Maxwell G.; Yin, C. Y.; Ram, Satyendra P.; Sullivan, Patrick A.

doi:10.1139/m80-004

Cited by 164 publications

(103 citation statements)

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“…Lee's, Lee's medium as described (Lee et al, 1975). Proline, imidazole buffer with proline as described (Shepherd et al, 1980). GlcNAc, imidazole buffer with N-acetylglucosamine, as described (Shepherd et al, 1980).…”

Section: Discussionmentioning

confidence: 99%

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Filamentous growth of Candida albicans in response to physical environmental cues and its regulation by the unique CZF1 gene

Brown

Giusani

Chen

et al. 1999

Molecular Microbiology

250

248

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Hyphal growth in the opportunistic fungal pathogen Candida albicans is believed to contribute to the virulence of the organism by promoting penetration of fungal cells into host tissue. In this study, stimulation of hyphal growth by a feature of the physical environment was demonstrated. Specifically, growth of cells embedded within a matrix promoted the formation of hyphae. The CZF1 gene, encoding a putative transcription factor, was shown to be involved in the regulation of hyphal growth under certain conditions, including embedded conditions. Ectopic expression of CZF1 in embedded cells promoted the rapid formation of hyphae. Elimination of CZF1 and CPH1, encoding a homologue of the Saccharomyces cerevisiae Ste12p transcription factor, led to a pronounced defect in filamentous growth of embedded cells. Elimination of CZF1 alone led to a moderate defect in hyphal growth under some conditions, including embedded conditions. Hyphal morphogenesis in response to matrix embedding may occur in the opportunistic pathogen, C. albicans, to promote invasion of fungal cells into host tissue.

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Section: Discussionmentioning

confidence: 99%

“…Proline, imidazole buffer with proline as described (Shepherd et al, 1980). GlcNAc, imidazole buffer with N-acetylglucosamine, as described (Shepherd et al, 1980). b. , Phenotype of wild-type strain.…”

Section: Discussionmentioning

confidence: 99%

Filamentous growth of Candida albicans in response to physical environmental cues and its regulation by the unique CZF1 gene

Brown

Giusani

Chen

et al. 1999

Molecular Microbiology

250

248

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“…Batch cultures were grown in defined medium of glucose, salts and biotin (GSB) (Shepherd et al, 1980) or yeast nitrogen base (YNB) (Difco) with glucose, at 30 "C in a rotary shaker. A chemostat with a capacity of 910 ml was used for continuous culture of C. albicans ATCC 10261.…”

Section: Methodsmentioning

confidence: 99%

Ammonium Assimilation by Candida albicans and Other Yeasts: Evidence for Activity of Glutamate Synthase

et al. 1989

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Activities and properties of the ammonium assimilation enzymes NADP+-dependent glutamate dehydrogenase (GDH), glutamate synthase (GOGAT) and glutamine synthetase (GS) were determined in batch and continuous cultures of Candida albicans. NADP+-dependent GDH activity showed allosteric kinetics, with an So.s for 2-oxoglutarate of 7.5 mM and an apparent K , for ammonium of 5-0 mM. GOGAT activity was affected by the buffer used for extraction and assay, but in phosphate buffer, kinetics were hyperbolic, yielding K , values for glutamine of 750 p~ and for 2-oxoglutarate of 65 p~. The enzymes GOGAT and NADP+-dependent GDH were also assayed in batch cultures of Saccharomyces cerevisiae and three other pathogenic Candida spp. : Candida tropicalis, Candida pseudotropicalis and Candida parapsilosis. Evidence is presented that GSlGOGAT is a major pathway for ammonium assimilation in Candida albicans and that this pathway is also significant in other Candida species.

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“…High temperature (ϭ37°C), neutral pH (ϭ6.5) and the addition of serum induced hyphal growth [1,11,13]. We examined the effect of these factors on hyphal growth inhibition by valinomycin (Table 1).…”

Section: Effect Of Other Factors Causing Hyphal Growth Induction On Hmentioning

confidence: 99%

“…Candida albicans is a major opportunistic fungal pathogen that has the ability to switch reversibly between yeast and hyphal forms depending on the environmental conditions [1]. It is thought that this ability is related to pathogenicity, which is reduced by the inhibition of hyphal growth, because morphological mutants defective in hyphal growth exhibit low virulence compared to the parental strain [2].…”

Section: Introductionmentioning

confidence: 99%

Relationship between Cell Morphology and Intracellular Potassium Concentration in Candida albicans

et al. 2006

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Previously we reported that valinomycin inhibited hyphal growth and induced growth as a chain of yeast cells under hyphal growth induction conditions in Candida albicans. To elucidate the hyphal growth inhibition by valinomycin, we examined the effect of various chemicals on the morphology and found that miconazole inhibited hyphal growth as well as valinomycin: both compounds promoted the leakage of potassium from cells. Analysis of intracellular potassium suggested that hyphal cells contain potassium at high concentrations in comparison with yeast cells. Hyphal growth inhibition by valinomycin was obstructed by the addition of serum. Potassium measurement showed that the addition of serum causes an increase in intracellular potassium, suggesting that the obstruction by serum might be due to an increase in intracellular potassium. The above-mentioned results strongly suggest that the addition of valinomycin and miconazole decreased interacellular potassium and this decrease inhibited hyphal transition.

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Germ tube induction in Candida albicans

Cited by 164 publications

References 0 publications

Filamentous growth of Candida albicans in response to physical environmental cues and its regulation by the unique CZF1 gene

Filamentous growth of Candida albicans in response to physical environmental cues and its regulation by the unique CZF1 gene

Ammonium Assimilation by Candida albicans and Other Yeasts: Evidence for Activity of Glutamate Synthase

Relationship between Cell Morphology and Intracellular Potassium Concentration in Candida albicans

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