Germinação in vitro de murici-pequeno (Byrsonima intermedia A. Juss.)

Abstract

RESUMOO murici-pequeno (Byrsonima intermedia A. Juss.), espécie medicinal do Cerrado, apresenta dificuldade de propagação sexuada decorrente da baixa taxa de germinação e emergência lenta das plântulas em campo. Objetivou-se com o presente trabalho estudar aspectos da germinação in vitro de murici-pequeno mediante avaliação do efeito dos meios de cultura MS e WPM e diferentes concentrações de BAP. Frutos maduros foram coletados de populações naturais, dos quais sementes e embriões foram excisados e utilizados como explante. Pelos resultados, infere-se que os meios de cultura mais eficientes para a germinação in vitro de sementes e embriões são o MS e WPM 50%, sem sacarose, apresentando 60% e 100% de germinação, respectivamente. Observou-se também que não se faz necessária a adição de BAP na germinação in vitro de embriões. Termos para indexação:Germinação in vitro, murici-pequeno, plantas medicinais, Byrsonima intermedia. ABSTRACTThe murici-pequeno (Byrsonima intermedia A. Juss.) is a medicinal plant of cerrado that presents difficulties of sexual propagation due to a low rate of seed germination and plant emergency in the field. The objective of this work was study aspects of the in vitro germination of murici-pequeno evaluating the effects of MS and WPM culture media and different concentrations of BAP. Mature fruits were collected from native plants and their seeds and embryos excised and used as explants. The results indicated that the most effective culture media used for the in vitro seed and embryo germination were MS and WPM 50%, both with no sucrose, presenting 60 and 100% germination, respectively. The use of BAP is not required for in vitro embryo germination.

A method for seedling recovery in <i>Jatropha curcas</i> after cryogenic exposure of the seeds.

“…(Souza et al 2003) and Byrsonima intermedia A. Juss. (Nogueira et al 2004). Experiments with mature, immature and dry seeds of J. curcas also revealed that MS medium was efficient to sustain germination and continuous growth of plants under in vitro conditions (Nunes et al 2008).…”

Decontaminant solution on in vitro growth of Byrsonima intermedia seedlings

“…The culture medium used was WPM (LLOYD & McCOWN, 1980), with 50% of the salt concentration, no sucrose, 0.5 % agar and pH ajusted to 5.8 prior to autoclaving at 121°C for 20 minutes according to the protocol established by NOGUEIRA et al (2004); only one embryo was inoculated in each test tube (25x150mm) containing 10mL of WPM culture medium and each tube represented one replicate.…”

In vitro germination and acclimatization of cambui tree type seedlings

“…(NOGUEIRA et al, 2004), Cereus jamacaru DC. (RÊGO et al, 2009), Genipa americana L. (ALMEIDA et al, 2013) and exotic species Moringa oleifera Lam.…”