Germinação in vitro e desenvolvimento pós-seminal de plântulas de Pilosocereus aurisetus (Werderm.) Byles & G.D. Rowley (Cactaceae)

Abstract

In vitro germination and post-seminal development of plantlets of Pilosocereus aurisetus (Werderm.) Byles & G.D. Rowley (Cactaceae)Pilosocereus aurisetus is a species of cactus of economic and environmental importance, but the predatory exploitation led the species to be listed as threatened with extinction. P. aurisetus propagation occurs mainly in natural environment by sexual reproduction, though there is no record of germination, viability and morphology of postseminal seeds of this species. Thus, the objective of this study was to evaluate the germination and describe the morphology of post-seminal development of seeds of P. aurisetus. For this purpose, seeds stored at ambient conditions for 19 months were treated as follows: soaking in water for 24 hours, pre-cooling, soaking in solution of gibberellin in concentrations of 250 mg L -1 and 500 mg L -1 and a control. The seeds were germinated on MS medium for 30 days, whenRecebido para publicação em

Germinação e aclimatização de Melocactus sergipensis Taylor & Meiado

“…1E). Similar results were obtained by Reis et al (2012) in a study of the in vitro germination and post-seminal development of Pilosocereus aurisetus (kappa-la-lapa) seedlings, in which the development of the seedling followed a sequence similar to that observed in M. sergipensis. Germination began on the seventh day after inoculation, after which a translucent seedling emerged (Fig.…”

“…Germination rates were 82% in treatment 1 and 92% in treatment 2 (seeds soaked in GA 3 ), which were similar to that of the control (86%), which indicates that the pre-soaking of the seeds, whether in water or GA 3 , did not affect germination rates under the experimental conditions adopted in the present study. Reis et al (2012) obtained similar results in a study of Pilosocereus aurisetus, although germination rates did not exceed 80% in either the control group or the seeds soaked for 24 hours. In a study of the germination of Melocactus bahiensis in different substrates and at distinct temperatures, by contrast, Lone et al (2009) obtained low germination rates of between 45% and 48%, values approximately half those recorded in the present study.…”

“…This indicates that soaking the seeds in water for 6 hours had a physiological effect similar to that of soaking in 2 mg L -1 of GA 3 , and may have been at least as effective as the high concentrations of GA 3 used by Barbara et al (2015) and Marchi et al (2013). Reis et al (2012) found that presoaking Pilosocereus aurisetus seeds in GA 3 for 24 hours did not improve the in vitro germination rate in comparison with the control (90%), whereas soaking the seeds 250 mg L -1 GA 3 reduced the germination rate to 18% and soaking in 500 mg L -1 GA 3 reduced it to 13%.…”

ANÁLISE OPERACIONAL DA DERRUBADA DE ÁRVORES COM HARVESTER EM CORTE RASO DE POVOAMENTOS DE Pinus taeda L.

“…Acarez-Melandez et al, (1994) (CALDAS, 1998). A germinação in vitro tem sido utilizada para germinar sementes de espécies nativas com baixas porcentagens de germinação o que tem possibilitado o maior sucesso na obtenção de mudas (PÊGO et al, 2015, REIS et al, 2012.…”

Crescimento in vitro e ex vitro de melocactus zahntneri sob influência da fonte de luz e esterilização do meio de cultura.

“…Diferentes fatores podem ser estudados durante a germinação in vitro. Reis et al (2012) testaram o efeito de diferentes tratamentos pré-germinativos em sementes de Pilosocereus aurisetus armazenadas por 19 meses, obtendo maiores percentuais de germinação no tratamento controle ou quando utilizada a embebição em água por 24 horas. Já Dias et al (2013), investigando o efeito de diferentes reguladores de crescimento adicionados ao meio de cultura, verificaram que o maior número de sementes emergidas de Melocactus bahiensis foi obtido quando utilizada a concentração de 0,1 mg L -1 de ácido naftaleno acético; enquanto Resende et al (2009) obtiveram maior percentual de germinação in vitro de Melocactus glaucescens utilizando o meio com 25% da concentração dos sais de MS (MURASHIGE;SKOOG, 1962) e redução da sacarose.…”