Germinal center kinetics in lymph nodes of primed mice stimulated with complexed as opposed to free antigen

Buerki, H.; Kraft, Rainer; Hess, M. W.; Laissue, Jean A.; Cottier, H.; Stoner, Richard D.

doi:10.1016/0165-2478(89)90118-1

Cited by 5 publications

(2 citation statements)

References 23 publications

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“…Hollister K et al have found that the immunization with HIV-1 gp120 can also cause Tfh cell differentiation and GC B cells responses, elicit antigen-specific antibodies, but GC reaction may limit antigen-specific IgG secretion [37] . And, a viral superantigen may induce strong GC response [55] . Our findings also suggested that the modification of gp120 may decrease the limitation on specific Ab secretion by the strong Tfh response in the immunization with wild-type HIV-1 gp120.…”

Section: Discussionmentioning

confidence: 99%

An HIV-1 Envelope Immunogen with W427S Mutation in CD4 Binding Site Induced More T Follicular Helper Memory Cells and Reduced Non-Specific Antibody Responses

Tian

Wang

et al. 2014

PLoS ONE

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The CD4 binding site (CD4BS) of the HIV-1 envelope glycoprotein (Env) contains epitopes for broadly neutralizing antibody (nAb) and is the target for the vaccine development. However, the CD4BS core including residues 425-430 overlaps the B cell superantigen site and may be related to B cell exhaustion in HIV-1 infection. Furthermore, production of nAb and high-affinity plasma cells needs germinal center reaction and the help of T follicular helper (Tfh) cells. We believe that strengthening the ability of Env CD4BS in inducing Tfh response and decreasing the effects of the superantigen are the strategies for eliciting nAb and development of HIV-1 vaccine. We constructed a gp120 mutant W427S of an HIV-1 primary R5 strain and examined its ability in the elicitation of Ab and the production of Tfh by immunization of BALB/c mice. We found that the trimeric wild-type gp120 can induce more non-specific antibody-secreting plasma cells, higher serum IgG secretion, and more Tfh cells by splenocyte. The modified W427S gp120 elicits higher levels of specific binding antibodies as well as nAbs though it produces less Tfh cells. Furthermore, higher Tfh cell frequency does not correlate to the specific binding Abs or nAbs indicating that the wild-type gp120 induced some non-specific Tfh that did not contribute to the production of specific Abs. This gp120 mutant led to more memory Tfh production, especially, the effector memory Tfh cells. Taken together, W427S gp120 could induce higher level of specific binding and neutralizing Ab production that may be associated with the reduction of non-specific Tfh but strengthening of the memory Tfh.

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Section: Discussionmentioning

confidence: 99%

An HIV-1 Envelope Immunogen with W427S Mutation in CD4 Binding Site Induced More T Follicular Helper Memory Cells and Reduced Non-Specific Antibody Responses

Tian

Wang

et al. 2014

PLoS ONE

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“…The GC formation becomes detectable as early as three days after the B or two days after the T cell transfer, which is at least 24-48 h sooner than after a primary injection of nominal Ag into a normal animal , Langevoort et al, 1963, Jacobson and Thorbecke, 1968, Coico et al, 1983). The vSAg induced GC response resembles more that of a secondary response to nominal Ag, where the presence of primed T cells causes an acceleration in GC formation (Buerki et al, 1989). This rapidity is undoubtedly the result of the…”

Section: Discussionmentioning

confidence: 99%

Induction of Germinal Centers by MMTV EncodedSuperantigen on B Cells

Simmons

Simms

Chiarle

et al. 2001

Journal of Immunology Research

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It has not been established whether an endogenous superantigen (SAg) expressed on B cells can induce germinal centers (GCs). An interesting model is that of mammary tumor virus encoded viral SAgs, which induce vigorous T cell proliferation and are predominantly expressed on activated B cells. We have used this model to analyze the possibility that direct stimulation of Mtv7+ DBA/2 B cells by vSAg-responsive (Vβ6+) BALB/c T cells can give rise to GCs. Injection of BALB/c SCID mice iv with 2 × 106 DBA/2 B cells, together with LPS, followed by 2 × 106 BALB/c T cells induces numerous large splenic GCs within 3–5 days. The GCs are still large on day 7, but are very much reduced by day 10. B cell activation with LPS is needed for this effect. These GCs form in spite of the apparent absence of follicular dendritic cells (FDCs) as judged by staining for several FDC surface markers. Control mice receiving either BALB/c T or DBA/2 B cells + LPS alone or DBA/2 T + B cells + LPS fail to exhibit any GCs on days 3–7. Numerous small clusters of PNA+ cells, but few large GCs are observed when TNF-R(p55)-Ig is also injected, whereas LTβR-Ig treatment impeded the formation of aggregations of these cells even further, leaving scattered PNA+ single cells and very small clumps throughout the white pulp of the spleens. Anti-TNFα had no effect. These results suggest that endogenous vSAg mediated GC formation is independent of antigen trapping by FDCs.

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Induction and Recall of the Secondary Immune Response Entirely in Tissue Culture

Šterzl¹,

Milerová²,

Mackova³

et al. 1995

Advances in Experimental Medicine and Biology

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Germinal center kinetics in lymph nodes of primed mice stimulated with complexed as opposed to free antigen

Cited by 5 publications

References 23 publications

An HIV-1 Envelope Immunogen with W427S Mutation in CD4 Binding Site Induced More T Follicular Helper Memory Cells and Reduced Non-Specific Antibody Responses

An HIV-1 Envelope Immunogen with W427S Mutation in CD4 Binding Site Induced More T Follicular Helper Memory Cells and Reduced Non-Specific Antibody Responses

Induction of Germinal Centers by MMTV EncodedSuperantigen on B Cells

Induction and Recall of the Secondary Immune Response Entirely in Tissue Culture

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