Germinating fission yeast spores delay in G1 in response to UV irradiation

Nilssen, Esben A.; Synnes, Marianne; Tvegård, Tonje; Vebø, Heidi; Boye, Erik; Grallert, Beáta

doi:10.1186/1471-2121-5-40

Cited by 7 publications

(1 citation statement)

References 60 publications

(62 reference statements)

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“…A large number of spores with the lowest possible vegetative cell contamination are required in order to investigate specific spore developmental programs and physiology, such as the mechanisms for the breaking or the maintenance of dormancy, the resumption of cell cycle during germination, the cellular bases of stress resistance, and cell polarity maintenance (Bonazzi et al, 2014;Nilssen et al, 2004;Thevelein, den Hollander, & Shulman, 1984). The protocols that are currently available for yeast spore preparation rely on the lysis or inhibition of growth of vegetative cells, conditions that can be tolerated by spores and thus lead to their enrichment in a culture.…”

Section: Introductionmentioning

confidence: 99%

Purification of Yeast Spores to Investigate Their Dynamics of Activation

Plante

Landry

2020

CP Microbiology

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Germination is an important developmental process that supports resumption of growth in dormant spores. The study of the mechanisms underlying germination requires a pure spore population devoid of other cell types. This article describes the sporulation of wild Saccharomyces cerevisiae and Saccharomyces paradoxus strains, and the isolation and purification of ascospores. We also describe a method to synchronously induce germination in a spore population as well as to measure spore activation. This procedure can be applied, for example, to the study of environmental conditions that trigger germination.

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Section: Introductionmentioning

confidence: 99%

Purification of Yeast Spores to Investigate Their Dynamics of Activation

Plante

Landry

2020

CP Microbiology

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Checkpoint Regulation of DNA Replication

Boye

Skjølberg²,

Grallert³

2009

Methods in Molecular Biology

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Molecular and physiological effects of environmental UV radiation on fungal conidia

et al. 2015

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Conidia are specialized structures produced at the end of the asexual life cycle of most filamentous fungi. They are responsible for fungal dispersal and environmental persistence. In pathogenic species, they are also involved in host recognition and infection. Conidial production, survival, dispersal, germination, pathogenicity and virulence can be strongly influenced by exposure to solar radiation, although its effects are diverse and often species dependent. UV radiation is the most harmful and mutagenic waveband of the solar spectrum. Direct exposure to solar radiation for a few hours can kill conidia of most fungal species. Conidia are killed both by solar UV-A and UV-B radiation. In addition to killing conidia, which limits the size of the fungal population and its dispersion, exposures to sublethal doses of UV radiation can reduce conidial germination speed and virulence. The focus of this review is to provide an overview of the effects of solar radiation on conidia and on the major systems involved in protection from and repair of damage induced by solar UV radiation. The efforts that have been made to obtain strains of fungi of interest such as entomopathogens more tolerant to solar radiation will also be reviewed.

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Germinating fission yeast spores delay in G1 in response to UV irradiation

Cited by 7 publications

References 60 publications

Purification of Yeast Spores to Investigate Their Dynamics of Activation

Purification of Yeast Spores to Investigate Their Dynamics of Activation

Checkpoint Regulation of DNA Replication

Molecular and physiological effects of environmental UV radiation on fungal conidia

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