Germination and short-term storage of Hippophae rhamnoides L. seeds and its ex-situ reintroduction potential assessment under North East Indian conditions

Abstract

Germination efficiency of freshly harvested Hippophae rhamnoides seeds collected from cold desert of Ladakh outside its native place under in vivo glass house condition recorded a highest of 73% germination in soilrite at 22±2°C and 60-70% RH within three weeks at Guwahati, North East India. Germination of seeds was significantly enhanced to a maximum of 90% under in vitro condition in 1/4 th Murashige & Skoog (MS) medium supplemented with 3% sucrose and 2% activated charcoal within two weeks. Glass house acclimatized healthy seedlings after been introduced to natural climatic conditions of Shillong and Guwahati of North East India showed inadequate survivability. Unlike storage at room temperature which is detrimental for seed viability, low temperature storage under refrigeration and natural climatic condition of Ladakh retains viability for prolonged period.

A review on genetic diversity, micropropagation and transformations in the high-value medicinal plant of Himalayas-Seabuckthorn (Hippophae sp.)

“…But the length of radical and plumule was highest in control (white light) with an optimized temperature of 25°C [46,47] . Germination of H. rhamnoides under in vivo (soil-rite) and in vitro (1/4 MS medium) showed 73% and 90%, respectively [38] .…”

“…are listed in Table 1. 5% NaOH (10 min), 70% EtOH (1 min) Meristem/Shoot tips [31] 2 drops of Tween 20, 70% EtOH (5 min), 30% NaOCl (1hr) Seeds [36] 2% NaOCl+0.03% Tween 20 (30 min) and 70% EtOH (2 min) [12,37] Tween 20 (20 min), 0.5% Bavastin (20 min), 1-2% NaOCl, 0.2% HgCl2 (5 min) and 70% EtOH (3 min) [38] 0.1% Teepol, Ascorbic acid+ Ctric acid (2 hrs) in agitation, 2.5 mg/mL Tetracyclin (2 hrs) at low temp, 70% EtOH (4 min), 0.1% HgCl2 (6 min) Axillary buds [33] H. salicifolia 0.5% NaOCl (2 min) Seeds [39] 0.1% Tween 20+ 50% NaOCl (20 min), 1% Bavistin (1 hr), 70% EtOH (70 sec), 0.01% HgCl2 (3min) [24. 40] 70% EtOH, liquid detergent (5 min), 0.1% HgCl2 (3-5 min) [41] Teepol (5 min), 0.1% Bavistin (10 min), 4% NaOCl/ HgCl2 Nodal segments [34] Cultural medium and Growth conditions: The synthetic media with constituents of minerals, nutrients, and carbon sources, along with the plant growth regulators (PGRs), have been prepared for rapid and ultimate responses in propagation.…”

“…Acclimatization refers to the stepwise or orderly transfer of plantlets to soil and adapts as autotrophic. The rooted propagates, after specified days of in vitro growth, are shifted to soil-rite [38] , Sand: Vermicompost: Perlite (2:1:1) under 20°C [12] , Sand: Perlite: Soil (1:1:1) under 30±2°C with 85% humidity [36] , Soil: Farmyard Manure (3:1) [52] for 3-4 weeks.…”