Eragrostis curvula has been studied extensively due to its primary use in pastures and potential utilisation as a food source, however the role of E. curvula cultivars in soil nutrient cycling in nutrient-poor grassland ecosystems is understudied. This study investigated the effects of E. curvula cultivars on soil bacterial communities, associated enzyme activities, and soil characteristics in soils collected in grassland ecosystems in Heidelberg, Gauteng, South Africa. Soil bacterial communities were isolated and identified in pre-planting soils and post E. curvula harvest. Over four months, soil characteristics and enzyme activities were determined for pre-planting and post E. curvula harvest. There was an increase in the bacteria isolated from post-harvest soils compared to pre-planting soils for both cultivars in all study sites. Soil growing the cultivars showed a significant increase in the nitrate reductase activity across all study sites. An increase in β-glucosidase in July was only observed in Agpal soils across all study sites. Soil N concentrations and pH increased in all post-harvest soils. Potassium and P concentrations in post-harvest soils were lower than in pre-panting soils for both cultivars. The principal component analysis revealed that the Ermelo cultivar uniformly influences the soil characteristics and extracellular enzyme activities in all study sites, while the Agpal cultivar had varying responses. This study’s findings highlight how E. curvula cultivars shift the microbial profile over time while increasing N and pH in grassland ecosystem soils.