Germination Requirements of
            <i>Bacillus macerans</i>
            Spores

Sacks, L. E.; Thompson, P. A.

doi:10.1128/jb.105.3.739-746.1971

Cited by 10 publications

(5 citation statements)

References 17 publications

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“…Germination in spores of B. megaterium QM B1551 in response to D-glucose, D-mannose and Lalanine, while no germination in response to galactose was also reported by Hyatt and Levinson (1964), Racine et al (1979), Christie and Lowe (2007) in their work which is in line with the findings obtained in current investigation. Dglucose has also been proved to be a powerful germinant inducing rapid germination in spores of Bacillus macerans B70 (Sacks and Thompson 1971), Clostridium botulinum Type E (Ando 1971), while for spores of these bacteria, Dmannose was found effective only at increasing concentration levels. Ando (1971) also reported maximum germination in spores of Clostridium botulinum Type E with galactose.…”

Section: Fluorescent Staining Observationsmentioning

confidence: 99%

Role of stereospecific nature of germinants in Bacillus megaterium spores germination

et al. 2017

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The present study was undertaken with the objective to assess the effect of distinct stereoisomeric forms of nutrient germinants (selected sugars and amino acids) on the process of germination onset in dormant spores of MTCC 2949. In this respect, epimers of glucose and enantiomers of alanine were employed in current work. When supplemented with these stereoisomers, spores were found germinated only with d-glucose and d-mannose among epimers of glucose and only with l-alanine among enantiomers of alanine. Interestingly, germination in spores was observed to negligible extent with d-galactose and d-alanine. These findings were obtained on the basis of four type of germination assays, namely reduction in absorbance measured at 600 nm (≤5 to ≥30%), refractility examination (phase bright and dark), esterase assay [fluorescence units 0.455-94.62 (×10)] and fluorescent staining (fluorescent/non-fluorescent signals). Understanding of spores germination process and efficacy of different forms of germinants to trigger germination is of immense importance. It aids in development of sensing and sterilization indicating tools employing chiefly spores as biorecognition elements and in uncovering the mechanism of diseases, food contamination and spoilages resulting from the germination of spores. The findings of current work support the possibility to explore such germination mechanism by significantly giving the clue for potential existence of stereospecific receptor sites on the surface of spores. Perhaps, these sites can specifically differentiate and recognize stereoisomerically diverse forms of germinants for induction of germination.

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Section: Fluorescent Staining Observationsmentioning

confidence: 99%

Role of stereospecific nature of germinants in Bacillus megaterium spores germination

et al. 2017

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“…The rate of gennination of aerobic spores has been shown to be influenced by the concentrations of the germinating agents (3,19,22,29).…”

Section: Resultsmentioning

confidence: 99%

Characterization of germination and activation of Bdellovibrio bdellocysts

Tudor¹,

Conti²

1978

J Bacteriol

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A simple method of assaying germination of bdellocysts in liquid medium has been devised. Bdellocysts can be induced to genninate by any of eight L-amino acids or the monovalent cations K+ and NH4+. L-Glutamine was the best individual inducer of germination, although the resulting rate of germination was much slower than in a complex medium. The use of a defined germination medium containing L-glutame, KCl, and NH4Cl produced a faster rate of germination than did complex media. Bdellocysts germinated most rapidly at pH 8.0 and at 35°C and required aerobic conditions. Respiration ofbdeliocysts began to increase at 3 min after the addition of germinants. Germination was inhibited by respiratory-chain inhibitors and by inhibitors of macromolecular synthesis. When bdellocysts were heat shocked at sublethal temperatures for short periods, there was no effect on the rate of germination in the defined germination medium or in the complex medium. However, heat-shocked bdellocysts germinated at a much faster rate in the presence of single inducers of germination when compared to nonshocked bdeilocysts. The bdeflovibrios are predacious bacteria that are capable of growth on susceptible prey bacteria. One strain, Bdellovibrio sp. strain W (2),

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“…Diverse peptides were detected in B. subtilis trpC2 broth. Nicotinamide reportedly enhances growth of both Gram-negative and Gram-positive bacteria, such as M. avium, Propionibacterium acnes, S. aureus, and B. macerans [73][74][75][76]. Indole-3-carboxaldehyde was shown to efficiently inhibit biofilm formation by Vibrio cholerae O1 [77].…”

Section: Metabolome Analysis Of the Constituents In C Thermocellum Mv And B Subtilis Brothmentioning

confidence: 99%

Metabolome Analysis of Constituents in Membrane Vesicles for Clostridium thermocellum Growth Stimulation

2021

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The cultivation of the cellulolytic bacterium, Clostridium thermocellum, can have cost-effective cellulosic biomass utilizations, such as consolidated bioprocessing, simultaneous biological enzyme production and saccharification. However, these processes require a longer cultivation term of approximately 1 week. We demonstrate that constituents of the C. thermocellum membrane vesicle fraction significantly promoted the growth rate of C. thermocellum. Similarly, cell-free Bacillus subtilis broth was able to increase C. thermocellum growth rate, while several B. subtilis single-gene deletion mutants, e.g., yxeJ, yxeH, ahpC, yxdK, iolF, decreased the growth stimulation ability. Metabolome analysis revealed signal compounds for cell–cell communication in the C. thermocellum membrane vesicle fraction (ethyl 2-decenoate, ethyl 4-decenoate, and 2-dodecenoic acid) and B. subtilis broth (nicotinamide, indole-3-carboxaldehyde, urocanic acid, nopaline, and 6-paradol). These findings suggest that the constituents in membrane vesicles from C. thermocellum and B. subtilis could promote C. thermocellum growth, leading to improved efficiency of cellulosic biomass utilization.

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Germination Requirements of Bacillus macerans Spores

Cited by 10 publications

References 17 publications

Role of stereospecific nature of germinants in Bacillus megaterium spores germination

Role of stereospecific nature of germinants in Bacillus megaterium spores germination

Characterization of germination and activation of Bdellovibrio bdellocysts

Metabolome Analysis of Constituents in Membrane Vesicles for Clostridium thermocellum Growth Stimulation

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