Germination, α-, β-Amylase and Total Dehydrogenase Activities of Amaranthus Caudatus Seeds Under Water Stress in the Presence of Ethephon or Gibberellin A3

Białecka, Bożena; Kępczyński, Jan

doi:10.2478/v10182-010-0001-0

Cited by 21 publications

(24 citation statements)

References 24 publications

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“…The sequence of events leading to seed germination and root emergence is governed by water uptake from the external medium (Kaur et al 1998;Hodge et al 2009). Water availability plays a significant role in enzymatic reactions, solubilisation and transportation of metabolites, and also as a reagent in the hydrolytic breakdown of proteins, lipids, and carbohydrates in the storage tissues of germinating seeds (Bewley & Black 1994;Biaecka & Kępczyn´ski 2010). Amylase enzymes play an important role during seed germination, hydrolyzing the endosperm starch into metabolizable sugars, which provide the energy for the growth of roots and shoots (Nauriere et al 1992).…”

Section: Introductionmentioning

confidence: 99%

Effect of PEG-induced drought stress on seed germination of four lentil genotypes

Muscolo

Sidari

Anastasi

et al. 2013

Journal of Plant Interactions

185

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Seeds of four lentil genotypes (Castelluccio, Eston, Pantelleria, and Ustica) were subjected to five levels (0, 10, 15, 18, and 21%) of polyethylene glycol (PEG-6000). Germination percentage, root length, tissue water content (WC), a-and b-amylases, a-glucosidase activities, and osmolyte content were evaluated at 24, 48, and 72 h after starting the germination test. Water stress reduced seed germination percentage, root length, and seedling WC in all cultivars to different extent. The increase in proline content and total soluble sugars was greater for Eston and Castelluccio compared to the other genotypes. The activity of the enzymes involved in the germination process decreased in all cultivars; the activities of a-amylase and a-glucosidase were most negatively affected by osmotic stress, mainly in the drought sensitive Ustica and Pantelleria. Overall, Eston and Castelluccio were able to express greater drought tolerance and consequently could be used as a valuable resource for breeding programs.

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Section: Introductionmentioning

confidence: 99%

Effect of PEG-induced drought stress on seed germination of four lentil genotypes

Muscolo

Sidari

Anastasi

et al. 2013

Journal of Plant Interactions

185

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“…Addition of salicylic acid, jasmonic acid or brassinolide during seed conditioning affected dehydrogenases activity in a greater extent. The greatest total activity of these enzymes was observed when seeds were conditioned for 2 days in 10 -8 or 10 -10 M of brassinolide to 15 % moisture content and then exposed to heat shock of 45°C for 2 h. Activity of dehydrogenases (respiratory enzymes) is considered as an index of tissue respiration and metabolism (Kasai et al 1998;Białecka and Kępczyński 2010;Farooq et al 2010). Presumably, an increase of the respiration rate due to brassinolide application improved sunflower chilling tolerance.…”

Section: Resultsmentioning

confidence: 99%

The effect of seed conditioning, short-term heat shock and salicylic, jasmonic acid or brasinolide on sunflower (Helianthus annuus L.) chilling resistance and polysome formation

2014

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The aim of this study was to develop the method for increasing resistance of sunflower seedlings 'Wielkopolski' to chilling. Seeds were conditioned at 25°C for 2 days in water to 15, 20 and 25 % moisture content or in salicylic or jasmonic acid in concentration of 10 -2 ; 10 -3 and 10 -4 M or brassinolide in concentration of 10 -6 ; 10 -8 and 10 -10 -15 % moisture content. After 2 days of incubation the conditioned seeds were heat shocked at 45°C for 0, 30, 60, 120 and 240 min and 5 mm seedlings were exposed to chilling at 0°C for 21 days. The effectiveness of the methods was assessed by evaluation of roots growth in Phytotoxkit Microbiotest, changes in the activity of dehydrogenases, the integrity of the cytoplasmic membrane and formation of polysomes after seedling were returned to 25°C for 72 h. Seeds were conditioned at 25°C for 2 days in water to 15 % moisture content and then heat shocked at 45°C for 2 h decreased chilling injury of seedlings expressed by subsequent growth of the roots, electrolyte leakage, dehydrogenases activity and polysomes formation. Application of heat shock of 45°C for 2 h during seed conditioning additionally provided seedling protection against subsequent chilling conditions. Brasinolide, salicylic acid or jasmonic acid applied during seeds conditioning exhibited further beneficial effect on seedling resistance to chilling. The most pronounced effect was obtained due to seed conditioning to 15 % moisture content in solutions of brassinolide in concentration of 10 -8 M. After 2 days of imbibition treated in this way seeds were exposed to heat shock at 45°C for 2 h. The role of physiological events in improvement of sunflower chilling tolerance are discussed.

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“…Beyond this, the water works like reagent in the hydrolytic degradation of proteins, lipids, and carbohydrates (Białecka and Kępczyński, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…In the process of germination, it is necessary that the environment provides enough water for the activation of all metabolic events necessary for the resumption of embryonic axis growth (Bewley et al, 2013;Ataíde et al, 2014). The availability of water plays a significant role in enzymatic reactions, availability of reserves, transport of metabolites and participates in hydrolytic reactions of proteins, lipids, and carbohydrates (Białecka and Kępczyński, 2010).…”

Section: Introductionmentioning

confidence: 99%

Research Article Physiological and enzymatic alterations in sesame seeds submitted to different osmotic potentials.

et al. 2017

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ABSTRACT. With the imminence of global climate changes that affect the temperature and the rainfall uniformity, it is growing the concern about the adaptation of crops to the water deficit. Thus, the objective of this study was to evaluate alterations in physiological and enzymatic mechanisms during the germination process of sesame seeds under different water availability. To simulate the water restriction we used PEG6000, a high molecular weight molecule that does not penetrate the seed structure but allows different osmotic potentials. The treatments were -0.1, -0.2, and -0.3 MPa, and the control. Germination, first-count germination, germination velocity index, and length and dry mass of the hypocotyl and radicle were performed. The seeds were weighed before and after treatments every 3 h. After each weighing, 100 seeds were taken for analysis of the enzymes alcohol dehydrogenase (ADH), malate dehydrogenase, esterase, catalase (CAT), superoxide dismutase (SOD), isocitrate lyase (ICL), and glutamate dehydrogenase (GTDH). The statistical design was completely randomized with five replications. PEG6000 prolonged ADH activity during the beginning of germination, maintaining the anaerobic metabolism for longer. Subsequently, their activity was reduced, as well as ICL, favoring the deterioration of the seeds that take the time to germinate. Behavior was evidenced by the appearance of SOD, CAT, and GTDH isoforms after 24 h of imbibition when water restriction was imposed. Therefore, the PEG600 is efficient in simulating water deficit conditions in future scenarios of climate change, offering impotent information regarding the germination behavior of the plants under these conditions.

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Germination, α-, β-Amylase and Total Dehydrogenase Activities of Amaranthus Caudatus Seeds Under Water Stress in the Presence of Ethephon or Gibberellin A3

Cited by 21 publications

References 24 publications

Effect of PEG-induced drought stress on seed germination of four lentil genotypes

Effect of PEG-induced drought stress on seed germination of four lentil genotypes

The effect of seed conditioning, short-term heat shock and salicylic, jasmonic acid or brasinolide on sunflower (Helianthus annuus L.) chilling resistance and polysome formation

Research Article Physiological and enzymatic alterations in sesame seeds submitted to different osmotic potentials.

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