Gestational exposure to ziram disrupts rat fetal Leydig cell development

Liu, Jianpeng; Wang, Yiyan; Fang, Yinghui; Ni, Chaobo; Ma, Leikai; Zheng, Wenwen; Bao, Suhao; Li, Xiaoheng; Lian, Qingquan

doi:10.1016/j.chemosphere.2018.03.142

Cited by 16 publications

(9 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Importantly, available literature indicates spermatogenesis/oogenesis damage resulting from the exposure to pesticides, although at concentrations higher than the ones used here. For instance, oral exposure to ziram (1 -8 mg / kg/ day) disrupts fetal Leydig cell development and inhibits several steroidogenic enzymes, negatively affecting male reproduction (Guo et al, 2017;Liu et al, 2018). The neonicotinonid thiacloprid (22.5 mg / kg / day) impairs sperm quality, induces abnormal spermatozoa morphology and irregular seminiferous tubules (Kammoun et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Varying modalities of perinatal exposure to a pesticide cocktail elicit neurological adaptations in mice and zebrafish

Forner-Piquer

Klément

Gangarossa

et al. 2021

Environmental Pollution

View full text Add to dashboard Cite

Highlights 1) Perinatal pesticides links with long-term behavioral modifications in male mice.2) Behavioral changes are accompanied by electrographic brain slowing in vivo.3) In zebrafish larvae, pesticide cocktail elicited dose-dependent motor-behavioral changes.4) In zebrafish larvae, chlorpyriphos is a behavior-modifying pesticide contained in the cocktail.5) Non-lethal prenatal pesticide cocktail does not provoke brain malformations.

show abstract

Section: Discussionmentioning

confidence: 99%

Varying modalities of perinatal exposure to a pesticide cocktail elicit neurological adaptations in mice and zebrafish

Forner-Piquer

Klément

Gangarossa

et al. 2021

Environmental Pollution

View full text Add to dashboard Cite

show abstract

“…One male pup testis per litter was randomly selected and fixed in Bouin's solution for 24 h. The fixed testis was embedded in paraffin, and then assembled in a tissue array tray as described previously 21 . CYP11A1, a key enzyme for T synthesis, was used as a biomarker 21 to show FLCs in the fetal testes. SOX9, a key transcription factor of SCs, 22 was used to show SCs.…”

Section: Methodsmentioning

confidence: 99%

“…SOX9, a key transcription factor of SCs, 22 was used to show SCs. Antibody‐derived immunohistochemistry was performed using a Vector kit (Burlingame, CA) to identify FLCs and SCs as described 21 . In brief, the testis tissue‐array was cut into a series of 6 μm sections by a microtome.…”

Section: Methodsmentioning

confidence: 99%

“…Antibody-derived immunohistochemistry was performed using a Vector kit (Burlingame, CA) to identify FLCs and SCs as described. 21 In brief, the testis tissue-array was cut into a series of 6 μm sections by a microtome. The sections were dewaxed and subjected to sequential procedures of immunohistochemistry, including antigen presentation, endogenous peroxidase inhibition, primary antibody incubation, secondary antibody ligation, color staining, and counterstaining.…”

Section: Testis Tissue-array Setup and Immunohistochemistrymentioning

confidence: 99%

See 1 more Smart Citation

Perfluorotridecanoic acid inhibits fetal Leydig cell differentiation after in utero exposure in rats via increasing oxidative stress and autophagy

Zou

Yan

et al. 2021

Environmental Toxicology

Self Cite

View full text Add to dashboard Cite

Perfluorotridecanoic acid (PFTrDA) is a long-chain perfluoroalkyl substance, and its effect on the differentiation of fetal Leydig cells remains unclear. The objective of this study is to explore the effect of in utero PFTrDA exposure on the differentiation of fetal Leydig cells and investigate its underlying mechanisms. Pregnant Sprague-Dawley female rats were daily administered by gavage of PFTrDA at doses of 0, 1, 5, and 10 mg/kg from gestational day 14 to 21. PFTrDA had no effect on the body weight of dams, but significantly reduced the body weight and anogenital distance of male pups at birth at a dose of 10 mg/kg. PFTrDA significantly decreased serum testosterone levels as low as 1 mg/kg. PFTrDA did not affect fetal Leydig cell number, but promoted abnormal aggregation of fetal Leydig cells at doses of 5 and 10 mg/kg. PFTrDA down-regulated the expression of Insl3, Lhcgr, Scarb1, Star, Hsd3b1, Cyp17a1, Nr5a1, and Dhh as well as their proteins. PFTrDA lowered the levels of antioxidants (SOD1, CAT, and GPX1), induced autophagy as shown by increased levels of LC3II and beclin1, and reduced the phosphorylation of mTOR. In conclusion, PFTrDA inhibits the differentiation of fetal Leydig cells in male pups after in utero exposure mainly through increasing oxidative stress and inducing autophagy.

show abstract

“…We prepared a tissue array as previously described (21). We selected one testis per rat (6 rats per group), cut in 8 discs, of which two pieces were randomly selected.…”

Section: Methodsmentioning

confidence: 99%

Fibroblast Growth Factor 1 Promotes Rat Stem Leydig Cell Development

Chen

Wang

et al. 2019

Front. Endocrinol.

Self Cite

View full text Add to dashboard Cite

Fibroblast growth factor 1 (FGF1) is reported to be expressed in the testis. How FGF1 affects stem Leydig cell development remains unclear. Here, we report the effects of FGF1 on rat stem Leydig cell development in an ethane dimethane sulfonate (EDS)-treated model. FGF1 (100 ng/testis) significantly increased serum testosterone level, increased PCNA-positive Leydig cell percentage and Leydig cell number, but down-regulated the expression of Lhcgr, Star, Cyp11a1, Hsd3b1, Cyp17a1, and Hsd11b1 in Leydig cells per se, after its daily intratesticular injection from post-EDS day 14 for 14 days. Primary culture of the seminiferous tubules showed that FGF1 stimulated EdU incorporation to stem Leydig cells but blocked the differentiation into the Leydig cell lineage, possibly via FGFR1-mediated mechanism. In conclusion, FGF1 promotes stem Leydig cell proliferation but blocks its differentiation.

show abstract

Gestational exposure to ziram disrupts rat fetal Leydig cell development

Cited by 16 publications

References 34 publications

Varying modalities of perinatal exposure to a pesticide cocktail elicit neurological adaptations in mice and zebrafish

Varying modalities of perinatal exposure to a pesticide cocktail elicit neurological adaptations in mice and zebrafish

Perfluorotridecanoic acid inhibits fetal Leydig cell differentiation after in utero exposure in rats via increasing oxidative stress and autophagy

Fibroblast Growth Factor 1 Promotes Rat Stem Leydig Cell Development

Contact Info

Product

Resources

About