GFA-protein gene expression on the astroglia in cow and rat brains

Kitamura, Tadahisa; Nakanishi, Kazuo; Watanabe, Sachihiko; Endo, Yasuhisa; Fujita, Setsuya

doi:10.1016/0006-8993(87)90839-0

Cited by 46 publications

(14 citation statements)

References 11 publications

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“…The distribution of GFAP immunostaining in transgenic animals did not differ from that observed in control animals either during postnatal development or in the adult. In general, the localization of GFAP immunoreactivity in the developing and adult mice observed in this study was similar to that described in previous investigations (Kitamura et al, 1987;Faddis and Vijayan, 1988;Shetty and Philips, 1992).…”

Section: Immunoreactivity For Gfapsupporting

confidence: 88%

Sympathetic axons invade the brains of mice overexpressing nerve growth factor

Kawaja

Crutcher

1997

J. Comp. Neurol.

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Transgenic mice that overexpress nerve growth factor (NGF) in cells producing glial fibrillary acidic protein were used to determine whether sympathetic axons will invade the undamaged, postnatal mammalian brain. By using reverse transcriptase-polymerase chain reaction, NGF mRNA transgene expression was detectable in the hippocampi and cerebella of transgenic mice but not in age-matched, wild type mice. Elevated levels of NGF protein were detected in the hippocampi and cerebella of postnatal and adult transgenic animals as well as in conditioned media from transgenic cerebellar astrocytes in culture. The brains of these transgenic mice were found to contain postganglionic sympathetic fibers, as identified by their immunohistochemical staining for tyrosine hydroxylase and by their disappearance following superior cervical ganglionectomy. In the cerebellum, a robust plexus of sympathetic fibers was evident in the deep white matter and in the inferior cerebellar peduncles. These axons within the cerebellum were observed as early as 14 days after birth and dramatically increased in number with age. Sympathetic axons were also associated with the large blood vessels of the hippocampal fissure and were present within the hilar region of the dentate gyrus. NGF immunoreactivity was present within the sympathetic axons as well as within glial cells in the transgenic cerebellum and hippocampus. Wild type mice, however, lacked similar patterns of immunostaining. These results demonstrate that elevated expression of NGF in the intact mammalian brain results in the growth of sympathetic axons into the central nervous system in the absence of injury.

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Section: Immunoreactivity For Gfapsupporting

confidence: 88%

Sympathetic axons invade the brains of mice overexpressing nerve growth factor

Kawaja

Crutcher

1997

J. Comp. Neurol.

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“…Their specificities have been confirmed in previous papers for anti-GFAP (18,33) anti-S-100 (22). Anti-glutamine synthetase was raised in our laboratory by immunizing rabbits with purified sheep gluamine synthetase (Sigma, cat no.…”

Section: Immunohistochemistrysupporting

confidence: 58%

Differential response of three astrocyte-specific proteins to fimbrial transection of the rat brain: Immunohistochemical observations with antibodies against glial fibrillary acidic protein, glutamine synthetase and S-100 protein.

Tanaka¹,

Araki

Masuzawa

1991

Acta Histochem. Cytochem

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“…Not all astrocytes are stained by GFAP immunohistochemistry, however. Protoplasmic astrocytes are usually not stained; presumably their low intermediate filament content puts them below the threshold for immunohistochemical detection (Bignami et al, 1980;Duffy et al, 1980;Kitamura et al, 1987;Schechter et al, 1981). Astrocytes reacting to tissue injury contain increased numbers of intermediate filaments and stain strongly for GFAP (Bignami et al, 1980;Lindsay, 1986).…”

Section: Introductionmentioning

confidence: 97%

Patterns of gliosis in alzheimer's disease and aging cerebrum

Beach¹,

Walker²,

McGeer³

1989

Glia

302

150

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The distribution of astrocytic gliosis in Alzheimer's disease (AD) and aging cerebrum, as marked by immunoperoxidase staining for glial fibrillary acidic protein (GFAP), was examined in whole-hemisphere coronal sections. Cortical gliosis in AD had an obvious laminar pattern. There were two heavy bands of staining, one in layers II-III and another in layer V. Normal aging cases sometimes displayed considerable cortical gliosis, but no specific patterns were apparent. Most AD cases, and some normal aging cases, displayed hypertrophy of immunoreactive astrocytes at grey matter-white matter interfaces, especially the cortico-medullary junction. Subcortical grey matter gliosis was common in both normal aging and AD, but there was no consistent pattern in either group. The deep cerebral white matter, which is stained evenly and heavily in young, healthy individuals, showed uneven staining in both normal elderly and AD brains. In both AD and aging, perivascular gliosis was prominent throughout the cerebrum and especially in the putamen. In conclusion, both AD and aging cerebri show extensive gliosis: AD cortical gliosis has a specific laminar pattern, but there does not appear to be an AD-specific pattern of subcortical gliosis.

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GFA-protein gene expression on the astroglia in cow and rat brains

Cited by 46 publications

References 11 publications

Sympathetic axons invade the brains of mice overexpressing nerve growth factor

Sympathetic axons invade the brains of mice overexpressing nerve growth factor

Differential response of three astrocyte-specific proteins to fimbrial transection of the rat brain: Immunohistochemical observations with antibodies against glial fibrillary acidic protein, glutamine synthetase and S-100 protein.

Patterns of gliosis in alzheimer's disease and aging cerebrum

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