GFP-Forked, a genetic reporter for studying<i>Drosophila</i>oocyte polarity

Baskar, Raju; Bakrhat, Anna

doi:10.1242/bio.039552

Cited by 10 publications

(8 citation statements)

References 34 publications

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“…This could be due to the efficacy of the RNAi-transgene, or suggestive of a yet-to-be identified negative regulator acting on PGRP-LB. Given previous characterization of the Mata-Gal4, we do not believe the finding is a result of GAL4 leakiness, as GFP expression is restricted to the ovary (Baskar et al 2019). However, transgene expression elsewhere in the adult or embryo not examined may potentially yield a phenotypic effect.…”

Section: Maternal Pgrp-lbmentioning

confidence: 77%

Maternal Priming of Offspring Immune System inDrosophila

Bozler

Kacsóh²,

Bosco

2020

G3 Genes|Genomes|Genetics

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Immune priming occurs when a past infection experience leads to a more effective immune response upon a secondary exposure to the infection or pathogen. In some instances, parents are able to transmit immune priming to their offspring, creating a subsequent generation with a superior immune capability, through processes that are not yet fully understood. Using a parasitoid wasp, which infects larval stages of Drosophila melanogaster, we describe an example of an intergenerational inheritance of immune priming. This phenomenon is anticipatory in nature and does not rely on parental infection, but rather, when adult fruit flies are cohabitated with a parasitic wasp, they produce offspring that are more capable of mounting a successful immune response against a parasitic macro-infection. This increase in offspring survival correlates with a more rapid induction of lamellocytes, a specialized immune cell. RNA-sequencing of the female germline identifies several differentially expressed genes following wasp exposure, including the peptiodoglycan recognition protein-LB (PGRP-LB). We find that genetic manipulation of maternal PGRP-LB identifies this gene as a key element in this intergenerational phenotype.

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Section: Maternal Pgrp-lbmentioning

confidence: 77%

Maternal Priming of Offspring Immune System inDrosophila

Bozler

Kacsóh²,

Bosco

2020

G3 Genes|Genomes|Genetics

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“…In Drosophila, expression of Nudel is required for structural integrity of the egg and for dorsoventral patterning of the embryo . Osp is the lepidopteran homolog of Nudel .…”

Section: Discussionmentioning

confidence: 99%

“…Oogenesis and development is a complex process regulated by multiple genes. In Drosophila , an organism where oogenesis and development are well understood, oogenesis begins with the asymmetric division of the germline stem cells . The Nudel gene is maternally required both for structural integrity of the egg and for dorsoventral patterning of the embryo .…”

Section: Introductionmentioning

confidence: 99%

Disruption of the ovarian serine protease (Osp) gene causes female sterility in Bombyx mori and Spodoptera litura

Wang

et al. 2019

Pest Management Science

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BACKGROUND Precise regulation of oogenesis is crucial to female reproduction. Seventy percent of pests belong to lepidopteran species, so it would be interesting to explore the highly conserved genes involved in oogenesis that do not affect growth and development in the lepidopteran model, Bombyx mori. This can provide potential target genes for pest control and promote the development of insect sterility technology. RESULTS In lepidopteran species, ovarian serine protease (Osp), which encodes a member of the serine protease family, is essential for oogenesis. In this study, we used transgenic CRISPR/Cas9 technology to obtain Osp mutants in the model lepidopteran insect Bombyx mori and in the lepidopteran agricultural pest Spodoptera litura. Sequence analysis of mutants revealed an array of deletions in Osp loci in both species. We found that the deletion of Osp resulted in female sterility, whereas male fertility was not affected. Although B. mori and S. litura mutant females mated normally, they laid fewer eggs than wild‐type females and eggs did not hatch. CONCLUSION Osp is crucial for female reproductive success in two species of Lepidoptera. As the Osp gene is highly conserved in insect species, this gene is a potential molecular target for genetic‐based pest management. © 2019 Society of Chemical Industry

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“…Graydon Gonsalvez, Augusta University, targeting Lis1 CDS 1197-1217 nt, 5'-TAGCGTAGATCAAACAGTAAA-3') 19 ; UAS-BicD-RNAi (TRiP.GL00325, attP2, III, Bloomington Drosophila Stock Center #35405, targeting BicD 3'UTR 639-659 nt, 5'-ACGATTCAGATAGATGATGAA-3'); UAS-Spindly-RNAi (TRiP.HMS01283, attP2, III, Bloomington Drosophila Stock Center #34933, targeting Spindly CDS 1615-1635 nt, 5'-CAGGACGCGGTTGATATCAAA-3') 61 ; UAS-hook-RNAi (TRiP.HMC05698, attP40, II, Bloomington Drosophila Stock Center #64663, targeting HOOK CDS 241-261 nt, 5'-TACGACTACTACAGCGACGTA-3'); UAS-GFP-RNAi (Bloomington Drosophila Stock Center #41551); UASp-tdMaple3-αtub84B (II) 29 ; UASp-EMTB-3XTagRFP (III) 30 ; mat αtub67C-EMTB-3XGFP-sqh 3'UTR (attP40, an unpublished gift from Yu-Chiun Wang lab, RIKEN Center for Biosystems Dynamics Research); UASp-GFP-Patronin (II) (from Dr. Uri Abdu, Ben-Gurion University of the Negev) 30,54,78 ; Jupiter-GFP (protein trap line ZCL2183, III) 27,28,32 ; UASp-LifeAct-TagRFP (III, 68E, Bloomington Drosophila Stock Center # 58714); UASp-F-Tractin-tdTomato (II, Bloomington Drosophila stock center #58989) 45 ; UAS-Zip-RNAi (TRiP.GL00623, attP40, II, Bloomington Drosophila Stock Center #37480, targeting Zip 3'UTR 36-56 nt, 5'-CAGGAAGAAGGTGATGATGAA-3'); hs-FLP [12] pUASp-GEM. GEM (PfV-GS-Sapphire) was cut from pCDNA3.1-pCMV-PfV-GS-Sapphire (Addgene plasmid # 116933; RRID:Addgene_116933) 44 , and inserted into the pUASp vector by NheI(5')/XbaI(3').…”

Section: Methodsmentioning

confidence: 99%

A novel mechanism of bulk cytoplasmic transport by cortical dynein in Drosophila ovary

Lü¹,

Lakonishok²,

Serpinskaya³

et al. 2022

eLife

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Cytoplasmic dynein, a major minus-end directed microtubule motor, plays essential roles in eukaryotic cells. Drosophila oocyte growth is mainly dependent on the contribution of cytoplasmic contents from the interconnected sister cells, nurse cells. We have previously shown that cytoplasmic dynein is required for Drosophila oocyte growth and assumed that it simply transports cargoes along microtubule tracks from nurse cells to the oocyte. Here we report that instead of transporting individual cargoes along stationary microtubules into the oocyte, cortical dynein actively moves microtubules within nurse cells and from nurse cells to the oocyte via the cytoplasmic bridges, the ring canals. This robust microtubule movement is sufficient to drag even inert cytoplasmic particles through the ring canals to the oocyte. Furthermore, replacing dynein with a minus-end directed plant kinesin linked to the actin cortex is sufficient for transporting organelles and cytoplasm to the oocyte and driving its growth. These experiments show that cortical dynein performs bulk cytoplasmic transport by gliding microtubules along the cell cortex and through the ring canals to the oocyte. We propose that the dynein-driven microtubule flow could serve as a novel mode of fast cytoplasmic transport.

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GFP-Forked, a genetic reporter for studyingDrosophilaoocyte polarity

Cited by 10 publications

References 34 publications

Maternal Priming of Offspring Immune System inDrosophila

Maternal Priming of Offspring Immune System inDrosophila

Disruption of the ovarian serine protease (Osp) gene causes female sterility in Bombyx mori and Spodoptera litura

A novel mechanism of bulk cytoplasmic transport by cortical dynein in Drosophila ovary

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