gga-miRNOME, a microRNA-sequencing dataset from chick embryonic tissues

Abstract

MicroRNAs (miRNAs) are small non-coding RNA molecules, with sizes ranging from 18 to 25 nucleotides, which are key players in gene expression regulation. These molecules play an important role in fine-tuning early vertebrate embryo development. However, there are scarce publicly available miRNA datasets from non-mammal embryos, such as the chicken (Gallus gallus), which is a classical model system to study vertebrate embryogenesis. Here, we performed microRNA-sequencing to characterize the early stages of trunk and limb development in the chick embryo. For this, we profiled three chick embryonic tissues, namely, Undetermined Presomitic Mesoderm (PSM_U), Determined Presomitic Mesoderm (PSM_D) and Forelimb Distal Cyclic Domain (DCD). We identified 926 known miRNAs, and 1,141 novel candidate miRNAs, which nearly duplicates the number of Gallus gallus entries in the miRBase database. These data will greatly benefit the avian research community, particularly by highlighting new miRNAs potentially involved in the regulation of early vertebrate embryo development, that can be prioritized for further experimental testing.

Regulation of mRNA and miRNA in the response to Salmonella enterica serovar Enteritidis infection in chicken cecum

“…miRNAs are small non-coding RNA molecules, which are key players in gene expression regulation [ 55 ]. 3′-UTRs of mRNA are primarily targeted by miRNA molecules that form complex gene regulatory networks [ 56 ].…”

The vertebrate Embryo Clock: Common players dancing to a different beat

“…Recent work from our group showed that different miRNA species are expressed in the PSM and in the forelimb distal cyclic domain. These tissues have very different EC periodicities (discussed below), which suggests that miRNAs may play a role in establishing different paces of the EC ( Duarte et al, 2022 ).…”