GH, GHRH, and PIT Genes Polymorphisms of Local Swamp Buffalo in Pandeglang District, Banten Province

Abstract

The molecular selection of the genes influencing growth qualities can be used to genetically improve the production of native swamp buffaloes. Local swamp buffalo (60 hds.) maintained by smallholders in Pandeglang Regency, Banten Province, were examined for the genetic polymorphism of the growing family (GH, GHRH, and PIT1) genes. By using restriction enzymes of MspI (GH gene), HaeIII (GHRH gene), and HinfI (PIT-1 gene), the genotyping of GH g.1547T>C, GHRH g.4666G>C, and PIT-1g.1256G>A SNPs was found by polymerase chain reaction-restriction fragment polymorphism (PCR-RFLP). Genotyping on the respective GH_g.1547T>C, GHRH_g.4666G>C, and PIT-1_g.1256G>A loci, however, resulted in only one type of genotype i.e. CC, GG, and AA with one type of allele i.e. C, G, and A successively. Therefore, the frequencies were 100% or monomorphic for those genotypes and alleles of each growth gene. Also, the PIC values of each locus were 0.00, as were the heterozygosity observation (Ho) and expectation (He) values. It is possible to suggest increasing the genotype frequency of the SNPs of the relevant growth genes that are favorably correlated with the growth traits and associated economic traits of the observed local swamp buffalo in this area.