GhbZIP30‐GhCCCH17 module accelerates corm dormancy release by reducing endogenous ABA under cold storage in Gladiolus

Abstract

Gladiolus hybridus is one of the most popular flowers worldwide. However, its corm dormancy characteristic largely limits its off‐season production. Long‐term cold treatment (LT), which increases sugar content and reduces abscisic acid (ABA), is an efficient approach to accelerate corm dormancy release (CDR). Here, we identified a GhbZIP30‐GhCCCH17 module that mediates the antagonism between sugars and ABA during CDR. We showed that sugars promoted CDR by reducing ABA levels in Gladiolus. Our data demonstrated that GhbZIP30 transcription factor directly binds the GhCCCH17 zinc finger promoter and activates its transcription, confirmed by yeast one‐hybrid, dual‐luciferase (Dual‐LUC), chromatin immunoprecipitation‐quantitative PCR (ChIP‐qPCR) and electrophoretic mobility shift assay (EMSA). GhCCCH17 is a transcriptional activator, and its nuclear localisation is altered by surcose and cytokinin treatments. Both GhbZIP30 and GhCCCH17 positively respond to LT, sugars, and cytokinin treatments. Silencing GhbZIP30 or GhCCCH17 resulted in delayed CDR by regulating ABA metabolic genes, while their overexpression promoted CDR. Taken together, we propose that the GhbZIP30‐GhCCCH17 module is involved in cold‐ and glucose‐induced CDR by regulating ABA metabolic genes.

Regulating bulb dormancy release and flowering in lily through chemical modulation of intercellular communication

“…As sugars are important energy sources for dormancy release [ 27 ], we initially observed the phenotypes of dormant lily bulblets following treatment with various concentrations of sucrose combined with different regulators. Compared to the control (30 g/L sucrose), a higher sucrose concentration (50 g/L) accelerated bulb dormancy release (Fig.…”

“…PD acts as an essential switch for the maintenance and release of plant dormancy [ 29 ]. Notably, soluble sugars, upregulated by low temperatures, can be transported via PD, contributing to the release of dormancy in geophytes, like Gladiolus hybridus [ 27 , 30 ]. In the SAM of poplar, the dynamic transformation (degradation and deposition) of callose controls the opening and closing of PD channels, thereby regulating the release and maintenance of dormancy [ 6 ].…”

“…LTCE downregulates ABA content and upregulates GAs and cytokinins, which play a role in regulating PD transport during dormancy release [ 31 , 34 , 40 – 42 ]. Soluble sugars are transported by both PD and sugar transporters to the SAM, where they serve as energy resources for cell division and differentiation, promoting dormancy release and plant development [ 27 , 43 ]. Here, we demonstrated BDM and DDG can partially replace low-temperature storage to promote bulb dormancy release, sprouting, plant growth, and flowering in lilies.…”

Auxin regulates bulbil initiation by mediating sucrose metabolism in Lilium lancifolium

“…Sucrose can antagonize the inhibitory effects of strigolactone (SL) on rice tillering [ 27 ]. Glucose, which is a metabolite of sucrose, accelerates bud growth by reducing endogenous abscisic acid (ABA) [ 28 ]. Trehalose 6-phosphate (T6P) derived from glucose interacts with SNF1-related protein kinase-1 complex to regulate plant carbon–nitrogen nutrient signaling [ 29 ].…”

Screening and Preliminary Identification of Asparagus officinalis Varieties under Low-Temperature Stress

“…Moreover, ABA is an important promoter of low temperature resistance-related gene expression in plants [17][18][19]. ABA treatment can improve the low-temperature resistance of Arabidopsis [20], wheat [21], strawberry [22], Gladiolus [23], and other plants.…”