GhENODL6 Isoforms from the Phytocyanin Gene Family Regulated Verticillium Wilt Resistance in Cotton

Abstract

Verticillium wilt (VW), a fungal disease caused by Verticillium dahliae, currently devastates cotton fiber yield and quality seriously, yet few resistance germplasm resources have been discovered in Gossypium hirsutum. The cotton variety Nongda601 with suitable VW resistance and high yield was developed in our lab, which supplied elite resources for discovering resistant genes. Early nodulin-like protein (ENODL) is mainly related to nodule formation, and its role in regulating defense response has been seldom studied. Here, 41 conserved ENODLs in G. hirsutum were identified and characterized, which could divide into four subgroups. We found that GhENODL6 was upregulated under V. dahliae stress and hormonal signal and displayed higher transcript levels in resistant cottons than the susceptible. The GhENODL6 was proved to positively regulate VW resistance via overexpression and gene silencing experiments. Overexpression of GhENODL6 significantly enhanced the expressions of salicylic acid (SA) hormone-related transcription factors and pathogenicity-related (PR) protein genes, as well as hydrogen peroxide (H2O2) and SA contents, resulting in improved VW resistance in transgenic Arabidopsis. Correspondingly, in the GhENODL6 silenced cotton, the expression levels of both phenylalanine ammonia lyase (PAL) and 4-coumarate-CoA ligase (4CL) genes significantly decreased, leading to the reduced SA content mediating by the phenylalanine ammonia lyase pathway. Taken together, GhENODL6 played a crucial role in VW resistance by inducing SA signaling pathway and regulating the production of reactive oxygen species (ROS). These findings broaden our understanding of the biological roles of GhENODL and the molecular mechanisms underlying cotton disease resistance.

Dynamic characteristics and functional analysis provide new insights into the role of GauERF105 for resistance against Verticillium dahliae in cotton

“…The ability to scavenge ROS and prevent oxidative damage is crucial for plant defense against necrotrophic pathogens like V. dahliae . Our findings are in line with previous studies demonstrating the importance of antioxidant defenses in governing cotton's interaction with V. dahliae [ 33 , 34 ]. The upregulation of antioxidant systems, controlled in part by defense-associated transcription factors like ERFs, is likely a key component of the enhanced tolerance we observed in the GauERF105 overexpression lines.…”

Unravelling the Functional Role of GthGAPC2 in Cotton's Defense Against Verticillium dahliae through Proteome

“…Comparatively, the overexpression lines exhibited lower MDA and H 2 O 2 levels, accompanied by higher SOD and POD activities when compared to wildtype plants (Figure 9d‐g). Our findings align with prior research that highlights the critical role of antioxidant defense in governing the interaction between cotton plants and V. dahliae (Dos Santos et al, 2022; Zhang et al, 2022). Additionally, DAB staining results revealed a reduced staining intensity in transgenic Arabidopsis leaves in contrast to the more pronounced staining observed in wildtype plants (Figure 9h).…”

Transcriptome Profiling of a Salt Excluder Hybrid Grapevine Rootstock ‘Ruggeri’ throughout Salinity

“…Similarly, the ZIFL1 is critical in regulating stomatal closure during stress in Arabidopsis [ 45 ]. Moreover, the ENODL genes, such as GhENODL6 , were shown to exhibit SA-induction under biotic stress and regulate the ROS production in cotton [ 46 ]. Furthermore, 3 out of 4 hormone-related genes are JA-related, especially the JAZ3 that showed strong induction at 2–48 h compared to 0 h. It reported that salinity adaptation in grapevine is highly correlated with tight control of JA and its isoleucine conjugate (JA-Ile) production and, hence, fine-tuning their signals [ 3 ].…”