GhN/AINV13 positively regulates cotton stress tolerance by interacting with the 14–3-3 protein

Chen, Baizhi; Wang, Xiaoyan; Lv, Jiaoyan; Ge, Meng-Jie; Qiao, Kaikai; Chen, Quanjia; Zhang, Kunpeng; Wang, Jingshun; Fan, Shiliang; Ma, Qin

doi:10.1016/j.ygeno.2020.11.026

Cited by 13 publications

(12 citation statements)

References 78 publications

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“…Z. Chen et al, 2021;Ma et al, 2021;. To further analyze the role of GhSTP18 under salt stress, we carried out a VIGS experiment to inhibit the expression of GhSTP18.…”

Section: Silencing Ghstp18 Enhances Tolerance To Salt Stressmentioning

confidence: 99%

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GhSTP18, a member of sugar transport proteins family, negatively regulates salt stress in cotton

Qiao

Wang

et al. 2023

Physiologia Plantarum

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The sugar transporter protein (STP) family has been shown to play important roles in plant growth, development, and stress response. However, it has not been studied in cotton compared to other major crops. In this study, we identified 90 STP genes from four cotton species, performed bioinformatic analysis, and focused on the role of GhSTP18 in salt stress. According to our results, cotton STP proteins were divided into four subgroups according to the phylogenetic tree. A synteny analysis suggested that whole‐genome duplication (WGD) and segmental duplication were key drivers in the expansion of the STP gene family. The transcriptomic data analysis showed that 29 GhSTP genes exhibited sink‐specific expression. Quantitative real time‐polymerase chain reaction (qRT‐PCR) analyses revealed that expression of GhSTP18 was induced by salt treatment, heat treatment, cold treatment, and drought treatment, and continuously increased during a salt stress time course. Notably, GhSTP18 encodes a plasma membrane‐localized galactose transporter. Suppression of GhSTP18 transcription by a virus‐induced gene silencing (VIGS) assay reduced sensitivity to salt stress in cotton, indicating that GhSTP18 negatively regulates plant salt tolerance. These results provide an important reference and resource for further studying and deploying STP genes for cotton improvement.

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“…Z. Chen et al, 2021;Ma et al, 2021;. To further analyze the role of GhSTP18 under salt stress, we carried out a VIGS experiment to inhibit the expression of GhSTP18.…”

Section: Silencing Ghstp18 Enhances Tolerance To Salt Stressmentioning

confidence: 99%

“…Z. Chen et al, 2021). A quantitative real-time polymerase chain reaction (qRT-PCR) was performed with the primers (Table S1).…”

Section: Plant Materials Treatments Rna Extraction and Qrt-pcrmentioning

confidence: 99%

GhSTP18, a member of sugar transport proteins family, negatively regulates salt stress in cotton

Qiao

Wang

et al. 2023

Physiologia Plantarum

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“…One of the broad functions of the 14‐3‐3 family proteins is binding to the phosphorylated residues of target proteins, which may alter the conformation, stability, activity, or the intracellular localization of client proteins (Gokirmak et al ., 2010). In plants, 14‐3‐3s might be involved in sugar metabolism and signalling by binding CINs, fructose‐2,6‐bisphosphatase, and class II TPSs (Kulma et al ., 2004; Harthill et al ., 2006; Gao et al ., 2014; Chen et al ., 2021). Remarkably, the dysfunctional INVAN6 Mei025 can interact with INVAN6 and all its counterparts, in the same way as wild‐type INVAN6 (Figs 6, S11).…”

Section: Discussionmentioning

confidence: 99%

Maize cytosolic invertase INVAN6 ensures faithful meiotic progression under heat stress

Huang

et al. 2022

New Phytologist

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Summary Faithful meiotic progression ensures the generation of viable gametes. Studies suggested the male meiosis of plants is sensitive to ambient temperature, but the underlying molecular mechanisms remain elusive. Here, we characterized a maize (Zea mays ssp. mays L.) dominant male sterile mutant Mei025, in which the meiotic process of pollen mother cells (PMCs) was arrested after pachytene. An Asp‐to‐Asn replacement at position 276 of INVERTASE ALKALINE NEUTRAL 6 (INVAN6), a cytosolic invertase (CIN) that predominantly exists in PMCs and specifically hydrolyses sucrose, was revealed to cause meiotic defects in Mei025. INVAN6 interacts with itself as well as with four other CINs and seven 14‐3‐3 proteins. Although INVAN6Mei025, the variant of INVAN6 found in Mei025, lacks hydrolytic activity entirely, its presence is deleterious to male meiosis, possibly in a dominant negative repression manner through interacting with its partner proteins. Notably, heat stress aggravated meiotic defects in invan6 null mutant. Further transcriptome data suggest INVAN6 has a fundamental role for sugar homeostasis and stress tolerance of male meiocytes. In summary, this work uncovered the function of maize CIN in male meiosis and revealed the role of CIN‐mediated sugar metabolism and signalling in meiotic progression under heat stress.

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“…stress resistance [21], signal transduction, and the biosynthesis of the secondary metabolite [22,23].…”

Section: Introductionmentioning

confidence: 99%

Genomic Identification and Expression Analysis of Acid Invertase (AINV) Gene Family in Dendrobium officinale Kimura et Migo

Liu

Luo

et al. 2023

Preprint

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Background Dendrobium officinale Kimura et Migo, a traditional Chinese orchid herb famous for its great horticultural and medicinal value, grows in an adverse habitat and suffers from abiotic or biotic stresses. Acid invertases (AINVs) are widely considered enzymes involved in regulating sucrose metabolism and here revealed to participate in responses to environmental stress in plants. The AINV gene family members have been identified and characterized in multiple plant genomes. However, detailed information about this gene family and their expression patterns are unknown in D. officinale despite their significance in polysaccharide biosynthesis. Results This study systematically analyzed the D. officinale genome and identified four DoAINV genes, classified into two subfamilies based on subcellular prediction and phylogenetic analysis. Comparison of gene structures and conserved motifs in DoAINV genes showed high conservation during their evolution. The conserved amino acids and domains of DoAINV proteins were characterized as pivotal for their function. Abiotic and biotic stress responses cis-elements were the most abundant motif in all DoAIVN genes, indicating they were stress-responsive. Furthermore, bioinformatics analysis of transcriptome data, validated by quantitative real-time reverse transcription PCR (qRT-PCR), revealed distinct organ-specific expression patterns of DoAINV genes in eight tissues and in response to abiotic stresses. Soluble sugar content and interaction network were examined to provide information on stress release and sucrose metabolism. Conclusions DoAINV genes are involved in various activities such as growth and development, stress response, and polysaccharide biosynthesis. The results provide valuable insights into the AINV family and will be helpful in further investigating DoAINV functions in D. officinale.

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GhN/AINV13 positively regulates cotton stress tolerance by interacting with the 14–3-3 protein

Cited by 13 publications

References 78 publications

GhSTP18, a member of sugar transport proteins family, negatively regulates salt stress in cotton

GhSTP18, a member of sugar transport proteins family, negatively regulates salt stress in cotton

Maize cytosolic invertase INVAN6 ensures faithful meiotic progression under heat stress

Genomic Identification and Expression Analysis of Acid Invertase (AINV) Gene Family in Dendrobium officinale Kimura et Migo

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